The expression of Lin28B was co-regulated by H3K4me2 and Wnt5a/β-catenin/TCF7L2

doi:10.1016/S2095-3119(20)63441-4

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (12): 3054-3064.DOI: 10.1016/S2095-3119(20)63441-4

所属专题：动物科学合辑Animal Science

收稿日期:2020-05-15 出版日期:2020-12-01 发布日期:2020-11-19

The expression of Lin28B was co-regulated by H3K4me2 and Wnt5a/β-catenin/TCF7L2

ZHANG Ya-ni^{1, 2}, HU Cai^{1, 2}, WANG Ying-jie^{1, 2}, ZUO Qi-sheng^{1, 2}, LI Bi-chun^{1, 2}

¹ Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China
² Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education, Yangzhou University, Yangzhou 225009, P.R.China

Received:2020-05-15 Online:2020-12-01 Published:2020-11-19
Contact: Correspondence ZUO Qi-sheng, Tel: +86-514-87997206, Fax: +86-514-87997206, E-mail: 006664@yzu.edu.cn; LI Bi-chun, Tel: +86-514-87977207, Fax: +86-514-87350440, E-mail: yubcli@yzu.edu.cn
About author:ZHANG Ya-ni, E-mail: ynzhang@yzu.edu.cn;
Supported by:
We thank the Experimental Poultry Farm of the Poultry Institute, Chinese Academy of Agricultural Sciences, for providing experimental materials. This work was supported by the Key Research and Development Program of China (2017YFE0108000), the National Natural Science Foundation of China (31872341, 31572390), and the High-Level Talent Support Program of Yangzhou University, China.

摘要/Abstract

Abstract:

Lin28A and Lin28B are homologous RNA-binding proteins that participate in the development of primordial germ cells. The mechanisms underlying expression and regulation of Lin28A have been well documented, but such information for Lin28B is limited. In this study, a fragment of the Lin28B promoter was cloned, the pEGFP-pLin28B vector was constructed. DF-1 chicken fibroblasts were transfected and the expression of green fluorescent protein (GFP) was measured. Furtherly, Lin28B promoter of different lengths fragments was cloned using the chromosome-walking method and the fragments were ligated into the PGL3-Basic vector, and transfected into DF-1 cells. Results of dual-luciferase reporter assay showed that the core of the Lin28B promoter was included in the sequence from –1 431 to –1 034 bp. The binding sites of the transcription factor TCF7L2 was showed within this sequence by bioinformatics analysis. The promoter activity of Lin28B was downregulated (P<0.05) when the TCF7L2 binding site was mutated. Further experiments suggested that Lin28B promoter activity responded to the activation or inhibition of Wnt signaling. Results of chromatin immunoprecipitation and quantitative PCR showed that β-catenin-TCF7L2 may be enriched in the Lin28B promoter core area. In vivo and in vitro activation or inhibition of Wnt signaling significantly up- or down-regulated (P<0.05) Lin28B expression. H3K4me2 enriched in the promoter of Lin28B, which affected the regulation of Wnt signaling to Lin28B. In conclusion, our results showed that H3K4me2 and Wnt5a/β-catenin/TCF7L2 were the positive regulators of Lin28B expression. Findings of this study may lay a theoretical foundation for illuminating the mechanism underlying Lin28B expression.

Key words: primordial germ cells , Lin28B , promoter , H3K4me2 , Wnt5a/β-catenin/TCF7L2

. [J]. Journal of Integrative Agriculture, 2020, 19(12): 3054-3064.

ZHANG Ya-ni, HU Cai, WANG Ying-jie, ZUO Qi-sheng, LI Bi-chun. The expression of Lin28B was co-regulated by H3K4me2 and Wnt5a/β-catenin/TCF7L2[J]. Journal of Integrative Agriculture, 2020, 19(12): 3054-3064.

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The expression of Lin28B was co-regulated by H3K4me2 and Wnt5a/β-catenin/TCF7L2

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