Transmission characteristics of Tomato chlorosis virus (ToCV) by Bemisia tabaci MED and its effects on host preference of vector whitefly

doi:10.1016/S2095-3119(18)62080-5

Journal of Integrative Agriculture ›› 2019, Vol. 18 ›› Issue (9): 2107-2114.DOI: 10.1016/S2095-3119(18)62080-5

所属专题：昆虫和植物互作合辑Insect and Plant Interact

收稿日期:2018-04-26 出版日期:2019-09-01 发布日期:2019-08-31

Transmission characteristics of Tomato chlorosis virus (ToCV) by Bemisia tabaci MED and its effects on host preference of vector whitefly

WEI Ke-ke, LI Jie, DING Tian-bo, LIU Tong-xian, CHU Dong

Key Lab of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, P.R.China

Received:2018-04-26 Online:2019-09-01 Published:2019-08-31
Contact: Correspondence CHU Dong, E-mail: chinachudong@sina.com
About author:WEI Ke-ke, E-mail: wkk0229@sina.com;
Supported by:
This research was supported by the National Key Research and Development Program of China (2016YFC1201200), the Taishan Mountain Scholar Constructive Engineering Foundation of Shandong, China (tsqn20161040) and the National Natural Science Foundation of China (31572064).

摘要/Abstract

Abstract:

The epidemiology of Tomato chlorosis virus (ToCV) in China is closely associated with its vector whitefly, Bemisia tabaci (Gennadius) MED. However, the transmission characteristics of ToCV by B. tabaci MED remain poorly understood. In this study, we analyzed: 1) the horizontal and vertical transmission of ToCV by B. tabaci MED whiteflies; 2) the acquisition of ToCV by male and female B. tabaci MED whiteflies after different feeding durations; 3) the transmission efficacy of viruliferous male and female B. tabaci MED whiteflies after different inoculation access periods (IAPs); 4) the retention of ToCV by viruliferous male and female B. tabaci MED whiteflies after a 48 h acquisition access period (AAP); and 5) the effects of ToCV on host choice of healthy or ToCV-infected tomato plant of viruliferous and non-viruliferous B. tabaci MED at different time points. Our results showed that: 1) viruliferous males could not transfer ToCV to non-viruliferous females, and vice versa, viruliferous females could not pass on ToCV to non-viruliferous males. ToCV could not be detected in the F₁ generation adults; 2) ToCV could be detected within 4.0% of females or males after a 20 min AAP; 3) ToCV could be detected in 33.3% of tomato plants inoculated by 10 viruliferous males or females with IAPs of 20 or 30 min; 4) the maximum retention time in females was 7 and 5 days in males; and 5) non-viruliferous B. tabaci MED did not show a preference for ToCV-infected tomato plants or healthy tomato plants. However, viruliferous B. tabaci MED whiteflies did prefer to settle on healthy tomato plants over ToCV-infected tomato plants. These findings will be helpful to better understand the epidemiology of the recently emerged plant virus, ToCV, in tomato fields in China.

Key words: Tomato chlorosis virus , Bemisia tabaci MED , transmission characteristics , preference

. [J]. Journal of Integrative Agriculture, 2019, 18(9): 2107-2114.

WEI Ke-ke, LI Jie, DING Tian-bo, LIU Tong-xian, CHU Dong . Transmission characteristics of Tomato chlorosis virus (ToCV) by Bemisia tabaci MED and its effects on host preference of vector whitefly[J]. Journal of Integrative Agriculture, 2019, 18(9): 2107-2114.

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Transmission characteristics of Tomato chlorosis virus (ToCV) by Bemisia tabaci MED and its effects on host preference of vector whitefly

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