Mapping and genetic validation of a grain size QTL qGS7.1 in rice (Oryza sativa L.)

doi:10.1016/S2095-3119(18)62113-6

Journal of Integrative Agriculture ›› 2019, Vol. 18 ›› Issue (8): 1838-1850.DOI: 10.1016/S2095-3119(18)62113-6

收稿日期:2018-04-04 出版日期:2019-08-01 发布日期:2019-08-02

Mapping and genetic validation of a grain size QTL qGS7.1 in rice (Oryza sativa L.)

XUE Pao¹, ZHANG Ying-xin¹, LOU Xiang-yang¹, ZHU Ai-ke^{1, 2}, CHEN Yu-yu^{1, 3}, SUN Bin^{1, 4}, YU Ping¹, CHENG Shi-hua¹, CAO Li-yong¹, ZHAN Xiao-deng¹

¹ Zhejiang Key Laboratory of Super Rice Research/State Key Laboratory of Rice Biology/China National Rice Research Institute, Hangzhou 310006, P.R.China
² Nanchong Academy of Agricultural Sciences, Nanchong 637000, P.R.China
³ National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R.China
⁴ Institute of Crop Breeding and Cultivation, Shanghai Academy of Agricultural Sciences, Shanghai 201403, P.R.China

Received:2018-04-04 Online:2019-08-01 Published:2019-08-02
Contact: Correspondence CAO Li-yong, Tel/Fax: +86-571-63370329, E-mail: caolycgf@mail.hz.zj.cn; ZHAN Xiao-deng, Tel/Fax: +86-571-63370265, E-mail: zhanxiaodeng@caas.cn
About author:XUE Pao, Tel/Fax: +86-571-63370606, E-mail: 18268126424 @163.com;
Supported by:
This work was supported by grants from the National Key Research and Development Program of China (2018YFD0100806), the Zhejiang Provincial Natural Science Foundation of China (LY18C130008), the National Natural Science Foundation of China (31521064), the Major Project of the Genetically Modified and National Key Transgenic Research Projects, China (2016ZX08001-002), the Super Rice Breeding Innovation Team and Rice Heterosis Mechanism Research Innovation Team of the Chinese Academy of Agricultural Sciences Innovation Project (CAAS-ASTIP-2013-CNRRI).

摘要/Abstract

Abstract:

Grain size is a major determinant of grain weight, which is one of the components of rice yield. The objective o this study was to identify novel, and important quantitative trait loci (QTLs) for grain size and weight in rice. QTLs were mapped using a BC₄F₄ population including 192 backcross inbred lines (BILs) derived from a backcross between Xiaolijing (XLJ) and recombinant inbred lines (RILs). The mapping population was planted in both Lingshui (Hainan, 2015) and Fuyang (Zhejiang, 2016), with the short- and long-day conditions, respectively. A total of 10 QTLs for grain length, four for grain width, four for the ratio of grain length to width, and 11 for grain weight were detected in at least one environment and were distributed across 11 chromosomes. The phenotypic variance explained ranged from 6.76–25.68%, 14.30–34.03%, 5.28–26.50%, and 3.01–22.87% for grain length, grain width, the ratio of grain length to width, and thousand grain weight, respectively. Using the sequential residual heterozygotes (SeqRHs) method, qGS7.1, a QTL for grain size and weight, was mapped in a 3.2-Mb interval on chromosome 7. No QTLs about grain size and weight were reported in previous studies in this region, providing a good candidate for functional analysis and breeding utilization.

Key words: rice (Oryza sativa L.) , quantitative trait loci , grain size , grain weight , residual heterozygote

. [J]. Journal of Integrative Agriculture, 2019, 18(8): 1838-1850.

XUE Pao1, ZHANG Ying-xin1, LOU Xiang-yang1, ZHU Ai-ke, CHEN Yu-yu, SUN Bin, YU Ping, CHENG Shi-hua, CAO Li-yong, ZHAN Xiao-deng .

Mapping and genetic validation of a grain size QTL qGS7.1 in rice (Oryza sativa L.)

[J]. Journal of Integrative Agriculture, 2019, 18(8): 1838-1850.

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Mapping and genetic validation of a grain size QTL qGS7.1 in rice (Oryza sativa L.)

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