Characterization of TaCOMT genes associated with stem lignin content in common wheat and development of a gene-specific marker

doi:10.1016/S2095-3119(18)61958-6

摘要/Abstract

Abstract:

Stem lignin content (SLC) in common wheat (Triticum aestivum L.) contributes to lodging resistance. Caffeic acid 3-O-methyltransferase (COMT) is a key enzyme involved in lignin biosynthesis. Characterization of TaCOMT genes and development of gene-specific markers could enable marker-assisted selection in wheat breeding. In the present study, the full-length genomic DNA (gDNA) sequences of TaCOMT genes located on chromosomes 3A, 3B, and 3D were cloned by homologous cloning. Two allelic variants, TaCOMT-3Ba and TaCOMT-3Bb, were identified and differed by a 222-bp insertion/deletion (InDel) in the 3´-untranslated region (3´-UTR). A co-dominant gene-specific marker based on this InDel was developed and designated as TaCOMT-3BM. A total of 157 wheat cultivars and advanced lines grown in four environments were used to validate the associations between allelic patterns and SLC. The SLC of cultivars with TaCOMT-3Ba was significantly (P<0.01) higher than that of those with TaCOMT-3Bb, and the marker TaCOMT-3BM could be effectively used in wheat breeding.

Key words: Triticum aestivum L. , caffeic acid 3-O-methyltransferase , stem lignin content , gene-specific marker , lodging resistance

. [J]. Journal of Integrative Agriculture, 2019, 18(5): 939-947.

FU Lu-ping, XIAO Yong-gui, YAN Jun, LIU Jin-dong, WEN Wei-e, ZHANG Yong, XIA Xian-chun, HE Zhong-hu. Characterization of TaCOMT genes associated with stem lignin content in common wheat and development of a gene-specific marker[J]. Journal of Integrative Agriculture, 2019, 18(5): 939-947.

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