Pectate lyase is a factor in the adaptability for Heterodera glycines infecting tobacco

doi:10.1016/S2095-3119(18)62090-8

Journal of Integrative Agriculture ›› 2019, Vol. 18 ›› Issue (3): 618-626.DOI: 10.1016/S2095-3119(18)62090-8

所属专题：线虫合辑Nematology

收稿日期:2018-07-15 出版日期:2019-03-01 发布日期:2019-03-07

Pectate lyase is a factor in the adaptability for Heterodera glycines infecting tobacco

TIAN Zhong-ling¹, SHI Hong-li^{1, 2}, Munawar Maria¹, ZHENG Jing-wu^{1, 3}

¹ Laboratory of Plant Nematology, Institute of Biotechnology, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China
² School of Resource and Environmental Sciences, Henan Institute of Science and Technology, Xinxiang 453003, P.R.China
³ Key Lab of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Hangzhou 310058, P.R.China

Received:2018-07-15 Online:2019-03-01 Published:2019-03-07
Contact: Correspondence ZHENG Jing-wu, Tel/Fax: +86-571-88982580, E-mail: jwzheng@zju.edu.cn
About author:TIAN Zhong-ling, E-mail: tzl@zju.edu.cn;
Supported by:
This research was supported by the Special Fund for Agro-scientific Research in the Public Interest in China (201503114).

摘要/Abstract

Abstract:

The soybean cyst nematode, Heterodeara glycines, is a serious pathogen of soybean, and reported to be the host of a wide range of Fabaceae. In the present study, the host specificity and reproductivity of two populations of H. glycines collected from soybean and tobacco were identified and characterized. The comparative identity between β-1,4-endoglucanase, pectate lyase and chorismate mutase of H. glycines parasitizing on soybean and tobacco were 99, 97 and 98%, respectively. The qRT-PCR analysis indicated that the expression of pectate lyase 2 gene was significantly higher in second-stage juveniles of H. glycines Henan population parasitizing on tobacco than that of H. glycines Shanxi population parasitizing on soybean. In addition, the pectic acid content of cell wall was significantly higher (45%) in the roots of tobacco than the roots of soybean. Our results indicate that the changes in transcript parasitism genes may be a result of long-term evolution illustrating how a plant-parasitic nematode adapts to the host environment for optimal infestation and survival.

Key words: Heterodera glycines , tobacco , parasitism genes , β-1,4-endoglucanase , pectate lyase , chorismate mutase , pectic acid

. [J]. Journal of Integrative Agriculture, 2019, 18(3): 618-626.

TIAN Zhong-ling, SHI Hong-li, Munawar Maria, ZHENG Jing-wu. Pectate lyase is a factor in the adaptability for Heterodera glycines infecting tobacco[J]. Journal of Integrative Agriculture, 2019, 18(3): 618-626.

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Pectate lyase is a factor in the adaptability for Heterodera glycines infecting tobacco

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