Kiwifruit (Actinidia chinensis) R1R2R3-MYB transcription factor AcMYB3R enhances drought and salinity tolerance in Arabidopsis thaliana

doi:10.1016/S2095-3119(18)62127-6

Journal of Integrative Agriculture ›› 2019, Vol. 18 ›› Issue (2): 417-427.DOI: 10.1016/S2095-3119(18)62127-6

收稿日期:2018-01-29 出版日期:2019-02-01 发布日期:2019-01-29

Kiwifruit (Actinidia chinensis) R1R2R3-MYB transcription factor AcMYB3R enhances drought and salinity tolerance in Arabidopsis thaliana

ZHANG Ya-bin^1*, TANG Wei^{1, 2*}, WANG Li-huan¹, HU Ya-wen1, LIU Xian-wen², LIU Yong-sheng^{1, 2}

¹ Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, P.R.China
² School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, P.R.China

Received:2018-01-29 Online:2019-02-01 Published:2019-01-29
Contact: Correspondence LIU Yong-sheng, Mobile: +86-18709832886, E-mail: liuyongsheng1122@hfut.edu.cn; TANG Wei, Mobile: +86-13339017320, E-mail: tangweitw@aliyun.com.cn
About author: ZHANG Ya-bin, Mobile: +86-18227630935, E-mail: kiwifruitactinidia@163.com; * These authors contributed equally to this study.
Supported by:
This work was financially supported by the National Natural Science Foundation of China (31471157 and 31700266).

摘要/Abstract

Abstract:

Kiwifruit is an important fruit crop that is highly sensitive to environmental stresses, such as drought, heat, cold, water logging and phytopathogens. Therefore it is indispensable to identify stress-responsive candidate genes in kiwifruit cultivars for the stress resistance improvement. Here we report the isolation and characterization of a novel kiwifruit R1R2R3-MYB homolog (AcMYB3R) whose expression was induced by drought, salinity and cold stress. In vitro assays showed that AcMYB3R is a nuclear protein with transcriptional activation activity by binding to the cis-element of the kiwifruit orthologue of G2/M phase-specific gene KNOLLE. The Arabidopsis transgenic plants overexpressing AcMYB3R showed drastically enhanced tolerance to drought and salt stress. The expressions of stress-responsive genes such as RD29A, RD29B, COR15A and RD22 were prominently up-regulated by ectopic expression of AcMYB3R. Our study provides a valuable piece of information for functional genomics studies of kiwifruit and molecular breeding in improving stress tolerance for crop production.

Key words: kiwifruit , R1R2R3-MYB , drought , abiotic stress

. [J]. Journal of Integrative Agriculture, 2019, 18(2): 417-427.

ZHANG Ya-bin, TANG Wei, WANG Li-huan, HU Ya-wen, LIU Xian-wen, LIU Yong-sheng. Kiwifruit (Actinidia chinensis) R1R2R3-MYB transcription factor AcMYB3R enhances drought and salinity tolerance in Arabidopsis thaliana[J]. Journal of Integrative Agriculture, 2019, 18(2): 417-427.

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Kiwifruit (Actinidia chinensis) R1R2R3-MYB transcription factor AcMYB3R enhances drought and salinity tolerance in Arabidopsis thaliana

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