Molecular characterization and functional analysis of two new lysozyme genes from soybean cyst nematode (Heterodera glycines)

doi:10.1016/S2095-3119(19)62766-8

Journal of Integrative Agriculture ›› 2019, Vol. 18 ›› Issue (12): 2806-2813.DOI: 10.1016/S2095-3119(19)62766-8

所属专题：线虫合辑Nematology

收稿日期:2019-04-25 出版日期:2019-12-01 发布日期:2019-12-23

Molecular characterization and functional analysis of two new lysozyme genes from soybean cyst nematode (Heterodera glycines)

WANG Ning¹, PENG Huan¹, LIU Shi-ming¹, HUANG Wen-kun¹, Ricardo Holgado², Jihong Liu-Clarke², PENG De-liang¹

¹ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
² NIBIO, Norwegian Institute of Bioeconomy Research, Pb 115, NO-1431 Ås, Norway

Received:2019-04-25 Online:2019-12-01 Published:2019-12-23
Contact: Correspondence Peng De-liang, E-mail: pengdeliang@caas.cn
About author:WANG Ning, E-mail: 954502700@qq.com;
Supported by:
This research was supported the Central Public-Interest Scientific Institution Basal Research Fund, China (Y2019GH03), the Special Fund for Agro-Scientific Research in the Public Interest of China (210503114), and SINOGRAIN II (CHN-17/0019): Technological Innovation to Support Environmentally-Friendly Food Production and Food Safety Under a Changing Climate-Opportunities and Challenges for Norway-China Cooperation.

摘要/Abstract

Abstract:

Soybean cyst nematode (SCN, Heterodera glycines (I.)) is one of the most important soil-borne pathogens for soybeans. In plant parasitic nematodes, including SCN, lysozyme plays important roles in the innate defense system. In this study, two new lysozyme genes (Hg-lys1 and Hg-lys2) from SCN were cloned and characterized. The in situ hybridization analyses indicated that the transcripts of both Hg-lys1 and Hg-lys2 accumulated in the intestine of SCN. The qRT-PCR analyses showed that both Hg-lys1 and Hg-lys2 were upregulated after SCN second stage juveniles (J2s) were exposed to the Gram-positive bacteria Bacillus thuringiensis, Bacillus subtilis or Staphylococcus aureus. Knockdown of the identified lysozyme genes by in vitro RNA interference caused a significant decrease in the survival rate of SCN. All of the obtained results indicate that lysozyme is very important in the defense system and survival of SCN.

Key words: soybean cyst nematode , lysozyme , Hg-lys1 , Hg-lys2 , innate defense against bacteria

. [J]. Journal of Integrative Agriculture, 2019, 18(12): 2806-2813.

WANG Ning, PENG Huan, LIU Shi-ming, HUANG Wen-kun, Ricardo Holgado, Jihong Liu-Clarke, PENG De-liang. Molecular characterization and functional analysis of two new lysozyme genes from soybean cyst nematode (Heterodera glycines)[J]. Journal of Integrative Agriculture, 2019, 18(12): 2806-2813.

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Molecular characterization and functional analysis of two new lysozyme genes from soybean cyst nematode (Heterodera glycines)

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