Application of virus-induced gene silencing for identification of FHB resistant genes

doi:10.1016/S2095-3119(18)62118-5

Journal of Integrative Agriculture ›› 2019, Vol. 18 ›› Issue (10): 2183-2192.DOI: 10.1016/S2095-3119(18)62118-5

• 论文 • 下一篇

收稿日期:2018-06-04 出版日期:2019-10-01 发布日期:2019-09-29

Application of virus-induced gene silencing for identification of FHB resistant genes

FAN Yan-hui^1*, HOU Bing-qian^1*, SU Pei-sen¹, WU Hong-yan², WANG Gui-ping¹, KONG Ling-rang¹, MA Xin¹, WANG Hong-wei¹

¹State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai’an 271018, P.R.China
² Shandong Agricultural University Fertilizer Science Technology Co., Ltd., Feicheng 271600, P.R.China

Received:2018-06-04 Online:2019-10-01 Published:2019-09-29
Contact: Correspondence WANG Hong-wei, Tel: +86-538-8249278, Fax: +86-538-8242226, E-mail: wanghongwei@sdau.edu.cn; MA Xin, Tel: +86-538-8249278, Fax: +86-538-8242226, E-mail: maxin138@163.com
About author:* These authors contributed equally to this study.
Supported by:
This work was supported by the National Natural Science Foundation of China (3315203911 and 31471488), the National Key Research and Development Program of China (2016YFD0100602), the Transgenic Special Item, China (2016ZX08002003-002 and 2016ZX08009-003), and the Bohai Granary Science and Technology Demonstration Project of Shandong Province, China (2017BHLC020).

摘要/Abstract

Abstract:

Virus-induced gene silencing (VIGS) showed several advantages to identify gene functions such as short experimental cycle, more broad hosts, etc. In this study, the feasibility and efficiency of employing Barley stripe mosaic virus (BSMV)-based VIGS system to evaluate Fusarium head blight (FHB) resistance were explored in wheat. With variable conditions tested, it showed that the maximal silencing efficiency 78% on spike was obtained when the recombinant BSMV was inoculated on flag leaf at flagging stage. However, the plant may reduce its own immunity to FHB when inoculated with BSMV. To induce this impact, different Fusarium graminearum strains were tested and SF06-1 strain was selected for FHB resistance evaluation. Using this system, TaAOC, TaAOS, and TaOPR3 involved in jasmonic acid (JA) signaling pathway were identified to positively regulate FHB resistance, which was underpinned by the results when silencing TaAOS in wheat by stable transgenic plants.

Key words: Triticeae , Fusarium head blight (FHB) , BSMV-VIGS , RNAi

. [J]. Journal of Integrative Agriculture, 2019, 18(10): 2183-2192.

FAN Yan-hui, HOU Bing-qian, SU Pei-sen, WU Hong-yan, WANG Gui-ping, KONG Ling-rang, MA Xin, WANG Hong-wei. Application of virus-induced gene silencing for identification of FHB resistant genes[J]. Journal of Integrative Agriculture, 2019, 18(10): 2183-2192.

参考文献

Baulcombe D C. 1999. Fast forward genetics based on virus-induced gene silencing. Current Opinion in Plant Biology, 2, 109–113.
Bennypaul H S, Mutti J S, Rustgi S, Kumar N, Okubara P A, Gill K S. 2012. Virus-induced gene silencing (VIGS) of genes expressed in root, leaf, and meiotic tissues of wheat. Functional & Integrative Genomics, 12, 143–156.
Bottalico A, Perrone G. 2002. Toxigenic Fusarium species and mycotoxins associated with head blight in small-grain cereals in Europe. European Journal of Plant Pathology, 108, 611–624.
Cahid C, Megane G, Stevenr S. 2010. Rapid determination of gene function by virus-induced gene silencing in wheat and barley. Crop Science, 50, 77–84.
Cakir C, Scofield S. 2008. Evaluating the ability of the Barley stripe mosaic virus-induced gene silencing system to simultaneously silence two wheat genes. Cereal Research Communications, 36, 217–222.
Campbell J, Huang L. 2010. Silencing of multiple genes in wheat using Barley stripe mosaic virus. Journal of Biotech Research, 2, 12–20.
Demircan T, Akkaya M. 2010. Virus induced gene silencing in Brachypodium distachyon, a model organism for cereals. Plant Cell Tissue and Organ Culture, 100, 91–96.
Ding L N, Xu H B, Yi H, Yang L, Kong Z, Zhang L, Xue S, Jia H, Ma Z. 2011. Resistance to hemi-biotrophic F. graminearum infection is associated with coordinated and ordered expression of diverse defense signaling pathways. PLoS ONE, 6, 4.
Ding X S, Schneider W L, Chaluvadi S R, Mian M A, Nelson R S. 2006. Characterization of a Brome mosaic virus strain and its use as a vector for gene silencing in monocotyledonous hosts. Molecular Plant-Microbe Interactions, 19, 1229–1239.
Fung F, Clark R F. 2004. Health effects of mycotoxins: A toxicological overview. Journal of Toxicology Clinical Toxicology, 42, 217–234.
Glazebrook J. 2005. Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annual Review of Phytopathology, 43, 205–227.
Gottwald S, Samans B, Lück S. 2012. Jasmonate and ethylene dependent defence gene expression and suppression of fungal virulence factors: two essential mechanisms of Fusarium head blight resistance in wheat. BMC Genomics, 13, 369.
Hein I, Barciszewska-Pacak M, Hrubikova K, Williamson S. 2005. Virus-induced gene silencing-based functional characterization of genes associated with powdery mildew resistance in barley. Plant Physioloy, 138, 2155–2164.
Holzberg S, Brosio P, Gros C, Pogue G P. 2002. Barley stripe mosaic virus induced gene silencing in a monocot plant. The Plant Journal, 30, 315–327.
Hou W, Mu J, Li A, Wang H, Kong L. 2015. Identification of a wheat polygala cturonase-inhibiting protein involved in Fusarium head bligght resistance. European Journal of Plant Pathology, 141, 731–745.
Liu Z H, Liu X L, Li B B. 2014. Copy number analysis of lipoxygenase RNAi gene (Loxi) in transgenic lines of wheat (Triticum aestivum). Journal of Agricultural Biotechnology, 22, 949–957. (in Chinese)
Jackson A O, Lim H S, Bragg J, Ganesan U, Lee M Y. 2009. Hordeivirus replication, movement, and pathogenesis. Annual Review of Phytopathology, 47, 385–422.
Kaiser B N, Siddiqi M Y. 2002. Functional analysis of an Arabidopsis T-DNA “knockout” of the high-affinity NH4+ transporter AtAMT1;1. Plant Physiology, 130, 1263–1275.
Kong L, Ohm H W, Anderson J M. 2007. Expression analysis of defense-related genes in wheat in response to infection by Fusarium graminearum. Genome, 50, 1038–1048.
Kumagai M H, Donson J, della-Cioppa G, Harvey D, Hanley K, Grill L K. 1995. Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA. Proceedings of the National Academy of Sciences of the United States of America, 92, 1679–1683.
Lee W S, Hammondkosack K E, Kanyuka K. 2012. Barley stripe mosaic virus-mediated tools for investigating gene function in cereal plants and their pathogens: Virus-induced gene silencing, host-mediated gene silencing, and virus-mediated overexpression of heterologous protein. Plant Physiology, 160, 582–590.
Li G L, Yang Y. 2008. Jasmonate and ethylene signaling pathway may mediate Fusarium head blight resistance in wheat. Crop Science, 48, 1888.
Ma M, Yan Y, Huang L, Chen M, Zhao H. 2012. Virus-induced gene-silencing in wheat spikes and grains and its application in functional analysis of HMW-GS-encoding genes. BMC Plant Biology, 12, 141.
Pacak A, Geisler K, Jorgensen B, Barciszewska-Pacak M, Nilsson L. 2010. Investigations of Barley stripe mosaic virus as a gene silencing vector in barley roots and in Brachypodium distachyon and oat. Plant Methods, 6, 26.
Pajerowska-Mukhtar K M, Mukhtar M S, Guex N, Halim V A, Rosahl S, Somssich I E, Gebhardt C. 2008. Natural variation of potato allene oxide synthase 2 causes differential levels of jasmonates and pathogen resistance in Arabidopsis. Planta, 228, 293–306.
Panwar V, McCallum B, Bakkeren G. 2013. Host-induced gene silencing of wheat leaf rust fungus Puccinia triticina pathogenicity genes mediated by the Barley stripe mosaic virus. Plant Molecular Biology, 81, 595–608.
Ragiba M, Vamsi N, Ratnesh C, Richard J, Alexis A, Sparks, Jyoti S. 2010. Involvement of salicylate and jasmonate signaling pathways in Arabidopsis interaction with Fusarium graminearum. Molecular Plant-Microbe Interactions, 23, 861–870.
Ratcliff F, Harrison B D, Baulcombe D C. 1997. A similarity between viral defense and gene silencing in plants. Science, 276, 1558–1560.
Renner T, Bragg J, Driscoll H E, Cho J, Jackson A O. 2009. Virus-induced gene silencing in the culinary ginger (Zingiber officinale): An effective mechanism for down-regulating gene expression in tropical monocots. Molecular Plant, 2, 1084–1094.
Ruiz M T, Voinnet O, Baulcombe D C. 1989. Initiation and maintenance of virus-induced gene silencing. The Plant Cell, 10, 937–946.
Schwach F, Vaistij F E, Jones L, Baulcombe D C. 2005. An RNA-dependent RNA polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal. Plant Physiology, 138, 1842–1852.
Scofield S R, Li H, Gill B S. 2005. Development of a virus-induced gene silencing system for hexaploid wheat and its use in functional analysis of the Lr21-mediated leaf rust resistance pathway. Plant Physiology, 138, 2165–2173.
Starkey D E, Ward T J, Aoki T, Gale L R, Kistler H C, Geiser D M, Suga H, Toth B, Varga J, O’Donnell K. 2007. Global molecular surveillance reveals novel Fusarium head blight species and trichothecene toxin diversity. Fungal Genetics and Biology, 44, 1191–1204.
Staswick P E, Yuen G Y, Lehman C C. 1998. Jasmonate signaling mutants of Arabidopsis are susceptible to the soil fungus Pythium irregulare. The Plant Journal, 15, 747–754.
Voinnet O, Lederer C, Baulcombe D C. 2000. A viral movement protein pre-vents spread of the gene silencing signal in Nicotiana benthamiana. Cell, 103, 157–167.
Wang X, Cao A, Yu C, Wang D, Wang X. 2010. Establishment of an effective virus induced gene silencing system with BSMV in Haynaldia villosa. Molecular Biology Reports, 37, 967–972.
Waterhouse P M, Wang M B, Lough T. 2001. Gene silencing as an adaptive defence against viruses. Nature, 411, 834–842.
Weigel D, Ahn J H, Blázquez M A, Borevitz J O, Christensen S K. 2000. Activation tagging in Arabidopsis. Plant Physiology, 122, 1003–1014.
Yuan C, Li C, Yan L, Jackson A O, Liu Z, Han C, Yu J, Li D. 2011. A high throughput Barley stripe mosaic virus vector for virus induced gene silencing in monocots and dicots. PLoS ONE, 6, e26468.
Zhang H B, Liu J W, Liu B J, Liu B, Liang Y C. 2013. Composition and pathogenicity differentiation of pathogenic species isolated from Fusarium head blight in Shandong Province. Acta Phytophylacica Sinica, 40, 27–32. (in Chinese)
Zhao Y, Dong W, Zhang N B, Ai X H, Wang M C, Huang Z G, Xiao L T, Xia G M. 2014. A wheat allene oxide cyclase gene enhances salinity tolerance via jasmonate signaling. Plant Physiology, 164, 1068–1076.

Application of virus-induced gene silencing for identification of FHB resistant genes

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics