Journal of Integrative Agriculture ›› 2018, Vol. 17 ›› Issue (2): 276-284.DOI: 10.1016/S2095-3119(17)61685-X

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  • 收稿日期:2017-02-20 出版日期:2018-02-20 发布日期:2018-02-01

A dCAPS marker developed from a stress associated protein gene TaSAP7-B governing grain size and plant height in wheat

WANG Yi-xue1, XU Qiao-fang2, CHANG Xiao-ping2, HAO Chen-yang2, LI Run-zhi1, JING Rui-lian2   

  1. 1 College of Agronomy, Shanxi Agricultural University, Taigu 030801, P.R.China
    2 National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
  • Received:2017-02-20 Online:2018-02-20 Published:2018-02-01
  • Contact: Correspondence LI Run-zhi, Tel: +86-354-6288373, E-mail: rli2001@hotmail.com; JING Rui-lian, Tel/Fax: +86-10-82105829, E-mail: jingruilian@caas.cn
  • About author:WANG Yi-xue, Mobile: +86-13466832889, E-mail: ciciwyx@163.com;
  • Supported by:

    This work was supported by the National Key Research and Development Program of China (2016YFD0100605) and the National Natural Science Foundation of China (31271720).

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Abstract: Stress associated proteins (SAPs) are the A20/AN1 zinc-finger proteins which confer to abiotic stresses in plants.  In this study, TaSAP7-B, including two AN1 domains, was isolated from B genome of wheat (Triticum aestivum L.).  Sequencing analysis on TaSAP7-B illustrated one InDel (insertion-deletion) and one SNP (single nucleotide polymorphism) in the promoter region while no diversity was observed in the coding region.  On the basis of SNP in the promoter region (–260 bp), a dCAPS (derived cleaved amplified polymorphic sequences) marker SNP-260 was developed for TaSAP7-B.  Using a natural population consisting of 262 wheat accessions, significant associations were detected between the marker SNP-260 and agronomic traits, such as plant height (PH), peduncle length (PL), length of penultimate internode (LPI), number of spike per plant (NSP), and 1 000-grain weight (TGW).  Two genotypes were identified using marker SNP-260 in the natural population.  Among them, the genotypes possessing C allele exhibited a higher TGW and shorter PH than the T genotypes.  Hence, base C was considered as the superior allele.  The dCAPS marker of TaSAP7-B can be instrumental for marker-assisted selection for high grain size and short plant height.  

Key words: Triticum aestivum L. ,  TaSAP7-B ,  single nucleotide polymorphism ,  association analysis ,  plant height ,  1 000-grain weight