Aphis gossypii is a globally distributed species and therefore has a highly variable life cycle.  Populations of A. gossypii in northern China exhibit greater genotypic diversity and a broader host range, yet the details of life cycles of different biotypes is still unclear.  In this study, the Cytb and 16S gene regions of A. gossypii collected from 5 common summer hosts and 4 primary hosts were analyzed.  A total of 57 haplotypes were obtained from 1 046 individual A. gossypii sequences.  The sequence included 44 variable sites, 27 of which were parsimony informative sites and 17 of which were singleton variable sites.  The most frequent 3 haplotypes were found in 896 individuals, representing a total of 85.7% of all individuals and 36 haplotypes were found in 1 individual.  A neighbor-joining tree was constructed using 21 haplotypes that were found in more than 2 individuals.  Considering the individual host plant, 5 biotypes were identified.  Type 1 corresponded exactly to the cucurbit host-race and the other 4 biotypes were found as cotton host-races.  Type 3 was the most abundant biotype in cotton fields in northern China.