Isolation and characterization of the secondary wall-related SND1 gene in hawthorn

doi:10.1016/S2095-3119(17)61791-X

Journal of Integrative Agriculture ›› 2018, Vol. 17 ›› Issue (09): 2007-2014.DOI: 10.1016/S2095-3119(17)61791-X

收稿日期:2017-09-12 出版日期:2018-09-01 发布日期:2018-08-14

Isolation and characterization of the secondary wall-related SND1 gene in hawthorn

CHEN Ke-qin¹, GUO Yun-na², SONG Meng-ru², DAI Hong-yan², ZHANG Zhi-hong¹

¹ Group of Molecular Biology of Fruit Trees, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, P.R.China
² Group of Fruit Germplasm Evaluation & Utilization, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, P.R.China

Received:2017-09-12 Online:2018-09-01 Published:2018-08-14
Contact: Correspondence DAI Hong-yan, E-mail: daihy1970@163.com; ZHANG Zhi-hong, E-mail: zhang_sau@163.com
Supported by:
This work was supported by the grants from the National Natural Science Foundation of China (31170635).

摘要/Abstract

Abstract:

Secondary wall-associated NAC domain protein1 (SND1) is a key regulator directly regulating the expression levels of MYB46 and MYB83 in the regulation network for secondary wall synthesis, especially in plant fibres. In this study, a SND1 gene was isolated from hawthorn (Crataegus pinnatifida) and named as CpSND1 because it has a conservative N-terminal DNA-binding domain with AtSND1. Arabidopsis plants overexpressing CpSND1 had similar phenotypes as plants overexpressing AtSND1, including inhibited growth, upward-curling leaves, sepal dysplasia and sterility. In addition, overexpressing CpSND1 in Arabidopsis also induced the expression of downstream genes, including lignin, cellulose and xylan biosynthesis genes as well as MYB genes. Our results provided functional information of CpSND1 for future genetic engineering in hawthorn.

Key words: hawthorn , transcription factor , SND1 , secondary cell wall , transcriptional regulation

. [J]. Journal of Integrative Agriculture, 2018, 17(09): 2007-2014.

CHEN Ke-qin, GUO Yun-na, SONG Meng-ru, DAI Hong-yan, ZHANG Zhi-hong. Isolation and characterization of the secondary wall-related SND1 gene in hawthorn[J]. Journal of Integrative Agriculture, 2018, 17(09): 2007-2014.

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Isolation and characterization of the secondary wall-related SND1 gene in hawthorn

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