A study on the pathogen species and physiological races of tomato Fusarium wilt in Shanxi, China

doi:10.1016/S2095-3119(18)61983-5

Journal of Integrative Agriculture ›› 2018, Vol. 17 ›› Issue (06): 1380-1390.DOI: 10.1016/S2095-3119(18)61983-5

所属专题：植物细菌真菌合辑Plant Bacteria/Fungus

收稿日期:2017-12-12 出版日期:2018-06-01 发布日期:2018-06-05

A study on the pathogen species and physiological races of tomato Fusarium wilt in Shanxi, China

CHANG Yin-dong¹, DU Bin1^{, 2}, WANG Ling³, JI Pei¹, XIE Yu-jie¹, LI Xin-feng¹, LI Zhi-gang^{1, 4}, WANG Jian-ming¹

¹ College of Agriculture, Shanxi Agricultural University, Taigu 030801, P.R.China
² Department of Horticulture, Taiyuan University, Taiyuan 030051, P.R.China
³ Technology Center Yuncheng Sub-Center, Shanxi Entry-Exit Inspection and Quarantine Bureau, Yuncheng 044000, P.R.China
⁴ Department of Genetics and Biochemistry, Clemson University, Clemson 29634-0318, South Carolina, USA

Received:2017-12-12 Online:2018-06-01 Published:2018-06-05
Contact: Correspondence WANG Jian-ming, Tel: +86-354-6289739, E-mail: jm.w@163.com
About author:CHANG Yin-dong, E-mail: cyd6398@163.com
Supported by:
This research was partially supported by the Shanxi Provincial Science and Technology Planning Project, China (20120311019-3), the Shanxi Provincial Science and Technology Foundation Platform Construction Project, China (1105-0104), and the Shanxi Provincial Graduate Education Innovation Project, China (2017BY065).

摘要/Abstract

摘要： Received 12 December, 2017 Accepted 26 February, 2018

Abstract:

In order to clarify the main pathogens of tomato Fusarium wilt in Shanxi Province, China, morphological identification, elongation factor 1 alpha (EF-1α) sequence analysis, specific primer amplification and pathogenicity tests were applied to study the isolates which were recovered from diseased plants collected from 17 different districts of Shanxi Province. The results were as follows: 1) Through morphological and molecular identification, the following 7 species of Fusarium were identified: F. oxysporum, F. solani, F. verticillioides, F. subglutinans, F. chlamydosporum, F. sporotrichioides, and F. semitectum; 2) 56 isolates of F. oxysporum were identified using specific primer amplification, among which, 29, 5 and 6 isolates were respectively identified as F. oxysporum f. sp. lycopersici physiological race 1, race 2, and race 3; 3) pathogenicity test indicated the significant pathogenicity of F. oxysporum, F. solani, F. verticillioides, and F. subglutinans to tomato plant. Therefore, among these 4 species confirmed as pathogenic to tomato in Shanxi, the highest isolation rate (53.3%) corresponded to F. oxysporum. Three physiological species, race 1, race 2, and race 3 of F. oxysporum f. sp. lycopersici are detected in Shanxi, among which race 1 is the most widespread pathogen and is also considered as the predominant race.

Key words:

tomato Fusarium wilt , Fusarium oxysporum f. sp. lycopersici , physiological races , EF-1&alpha, sequence analysis , specific primer amplification

. [J]. Journal of Integrative Agriculture, 2018, 17(06): 1380-1390.

CHANG Yin-dong, DU Bin, WANG Ling, JI Pei, XIE Yu-jie, LI Xin-feng, LI Zhi-gang, WANG Jian-ming. A study on the pathogen species and physiological races of tomato Fusarium wilt in Shanxi, China[J]. Journal of Integrative Agriculture, 2018, 17(06): 1380-1390.

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A study on the pathogen species and physiological races of tomato Fusarium wilt in Shanxi, China

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