Detection and characterization of an isolate of Tomato mottle mosaic virus infecting tomato in China

doi:10.1016/S2095-3119(17)61895-1

Journal of Integrative Agriculture ›› 2018, Vol. 17 ›› Issue (05): 1207-1212.DOI: 10.1016/S2095-3119(17)61895-1

• 论文 • 上一篇

收稿日期:2017-12-06 出版日期:2018-05-20 发布日期:2018-05-04

Detection and characterization of an isolate of Tomato mottle mosaic virus infecting tomato in China

ZHAN Bin-hui¹, CAO Ning¹, WANG Kai-na¹, ZHOU Xue-ping^{1, 2}

¹ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, China Academy of Agricultural Sciences, Beijing 100193, P.R.China
² State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China

Received:2017-12-06 Online:2018-05-20 Published:2018-05-04
Contact: Correspondence ZHOU Xue-ping, E-mail: xpzhou@ippcaas.cn, zzhou@zju.edu.cn
About author:ZHAN Bin-hui, E-mail: binhuizhan@126.com;
Supported by:
This research was supported by the Agricultural Science and Technology Innovation Program, China (ASTIP).

摘要/Abstract

摘要： Received 6 December, 2017 Accepted 23 January, 2018

© 2018 CAAS. Publishing services by Elsevier B.V. All rights reserved.
doi:

Abstract: Tomato (Solanum lycopersicum) plants exhibiting severe leaf distortion, mottle and systemic crinkling symptoms were identified in Hainan province in China in 2016. To survey and control the disease, it is necessary to identify and characterize the pathogen causing the disease. Dot enzyme-linked immunosorbent assay showed that the crude saps of the infected tomato samples reacted positively with the monoclonal antibody against Tobacco mosaic virus which indicated that one or more tobamoviruses are likely associated with the disease. RT-PCR and DNA sequence analysis results further elucidated that Tomato mottle mosaic virus (ToMMV) in Tobamovirus was the pathogen causing the mottle disease in tomato. We amplified and sequenced the full-length sequence of the genome which showed the highest nucleotide identity with ToMMV YYMLJ and ToMMV TiLhaLJ isolates. The putative virus isolate was named ToMMV Hainan. Biological indexing studies showed that ToMMV Hainan can infect Nicotiana benthamiana, Capsicum annuum and Solanum lycopersicum showing serious symptoms. This was the first identification and characterization of ToMMV infecting tomato in Hainan of China.

Key words: Tomato mottle mosaic virus , tomato , Tobamovirus , dot-ELISA , biological characterization

. [J]. Journal of Integrative Agriculture, 2018, 17(05): 1207-1212.

ZHAN Bin-hui, CAO Ning, WANG Kai-na, ZHOU Xue-ping. Detection and characterization of an isolate of Tomato mottle mosaic virus infecting tomato in China[J]. Journal of Integrative Agriculture, 2018, 17(05): 1207-1212.

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Detection and characterization of an isolate of Tomato mottle mosaic virus infecting tomato in China

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