Effects of thiamine on Trichothecium and Alternaria rots of muskmelon fruit and the possible mechanisms involved

doi:10.1016/S2095-3119(16)61584-8

Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (11): 2623-2631.DOI: 10.1016/S2095-3119(16)61584-8

收稿日期:2016-09-10 出版日期:2017-11-20 发布日期:2017-11-03

Effects of thiamine on Trichothecium and Alternaria rots of muskmelon fruit and the possible mechanisms involved

GE Yong-hong, LI Can-ying, LÜ Jing-yi, ZHU Dan-shi

College of Food Science and Engineering, Bohai University/Food Safety Key Laboratory of Liaoning Province/National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou 121013, P.R.China

Received:2016-09-10 Online:2017-11-20 Published:2017-11-03
Contact: Correspondence GE Yong-hong, E-mail: geyh1979@163.com
Supported by:
This study was supported by the National Natural Science Foundation of China (31401554), and the Doctoral Initial Funding of Bohai University, China (bsqd201405).

摘要/Abstract

Abstract: The effects of thiamine against pink and black spot rots caused by Trichothecium roseum and Alternaria alternata and modulation on the metabolism of reactive oxygen species (ROS) and phenylpropanoid pathway were investigated in this paper. In vitro test indicated that thiamine significantly inhibited mycelia growth and spore germination of T. roseum and A. alternata. Thiamine at 100 mmol L^–1 effectively inhibited lesion development of muskmelon fruit inoculated with T. roseum or A. alternata, enhanced production rate of O2^-. and H₂O₂ content, activities of catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) in muskmelon fruit. Thiamine also affect phenylpropanoid pathway in muskmelon fruit by increasing the activities of phenylalanine ammonia lyase (PAL) and peroxidase (POD), the content of total phenolic compounds, flavonoids and lignin. These results suggest that the effects of thiamine on pink and black spot rots in muskmelon fruits are associated with its direct fungitoxic against the pathogens and the modulation of O₂^-. and H₂O₂ production, eliminating enzymes and phenylpropanoid pathway.

Key words: induced resistance , thiamine , muskmelon fruit , antimicrobial activity , phenylpropanoid pathway

. [J]. Journal of Integrative Agriculture, 2017, 16(11): 2623-2631.

GE Yong-hong, LI Can-ying, Lü Jing-yi, ZHU Dan-shi. Effects of thiamine on Trichothecium and Alternaria rots of muskmelon fruit and the possible mechanisms involved[J]. Journal of Integrative Agriculture, 2017, 16(11): 2623-2631.

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Effects of thiamine on Trichothecium and Alternaria rots of muskmelon fruit and the possible mechanisms involved

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