Single-nucleotide polymorphisms, mapping and association analysis of 1-FFT-A1 gene in wheat

doi:10.1016/S2095-3119(16)61471-5

Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (04): 789-799.DOI: 10.1016/S2095-3119(16)61471-5

收稿日期:2016-05-09 出版日期:2017-04-04 发布日期:2017-04-07

Single-nucleotide polymorphisms, mapping and association analysis of 1-FFT-A1 gene in wheat

YUE Ai-qin^{1, 2}, LI Ang², MAO Xin-guo², CHANG Xiao-ping², LI Run-zhi¹, JING Rui-lian²

¹College of Agriculture, Shanxi Agricultural University, Taigu 030801, P.R.China
²Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Germplasm and Utilization, Ministry of Agriculture, Beijing 100081, P.R.China

Received:2016-05-09 Online:2017-04-04 Published:2017-04-07
Contact: JING Rui-lian, Tel/Fax: +86-10-82105829, E-mail: jingruilian@caas.cn
About author:YUE Ai-qin, E-mail: yueaiqinnd@126.com.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (31461143024), the National Major Project for Developing New Genetically Modified (GM) Crops of China (2016ZX08010005), and the Agricultural Science and Technology Innovation Program, China (ASTIP).

摘要/Abstract

Abstract: Fructans are major nonstructural carbohydrates in wheat (Triticum aestivum L.). Fructan 1-fructosyltransferase (1-FFT) is the key enzyme in fructan biosynthesis. In the present study, 96 sequence variants were detected in the 1-FFT-A1 gene among 26 wheat accessions including UR208, and 15 of them result in amino acid substitutions, forming four haplotypes. Two markers M39 and M2164 were developed based on the InDel21-39 and SNP-2164 polymorphisms to distinguish the three haplotypes in the 1-FFT-A1. 1-FFT-A1 was located on chromosome 4A using marker M2164 and was flanked by markers Xcwm27 and 6-SFT-A1. By association analysis using a natural wheat population consisted of 154 accessions, the results showed that the two markers were significantly associated with water-soluble carbohydrate (WSC) content in the lower internode stem and total stem at the early and middle grain filling stages, 1 000-grain weight (TGW) at different grain filling stages and peduncle length (PLE). Comparison of the effects of three haplotypes on agronomic traits indicated that TGW, PLE and total number of spikelets per spike (TNSS) were signi?cantly influenced by haplotypes. HapIII showed a significant positive effect on TGW, PLE and TNSS.

Key words: 1-FFT-A1, single nucleotide polymorphism, association analysis, haplotype, marker development, wheat

. [J]. Journal of Integrative Agriculture, 2017, 16(04): 789-799.

YUE Ai-qin, LI Ang, MAO Xin-guo, CHANG Xiao-ping, LI Run-zhi, JING Rui-lian. Single-nucleotide polymorphisms, mapping and association analysis of 1-FFT-A1 gene in wheat[J]. Journal of Integrative Agriculture, 2017, 16(04): 789-799.

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Single-nucleotide polymorphisms, mapping and association analysis of 1-FFT-A1 gene in wheat

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