Changes of chlorogenic acid content and its synthesis-associated genes expression in Xuehua pear fruit during development

doi:10.1016/S2095-3119(16)61496-X

Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (02): 471-477.DOI: 10.1016/S2095-3119(16)61496-X

收稿日期:2015-12-30 出版日期:2017-02-20 发布日期:2017-01-31

Changes of chlorogenic acid content and its synthesis-associated genes expression in Xuehua pear fruit during development

HE Jin-gang^{1, 2}, CHENG Yu-dou^{1, 2}, GUAN Jun-feng^{1, 2}, GE Wen-ya¹, ZHAO Zhe¹

¹Institute of Genetics and Physiology, Hebei Academy of Agricultural and Forestry Science, Shijiazhuang 050051, P.R.China
²Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051, P.R.China

Received:2015-12-30 Online:2017-02-20 Published:2017-01-31
Contact: GUAN Jun-feng, Tel: +86-311-87652118, Fax: +86-311-87652119, E-mail: junfeng-guan@263.net
Supported by:
This study was supported by the Specific Financial Funds of Hebei Province, China (494-0502-JSN-7FB3).

摘要/Abstract

Abstract: According to synthetic pathway of plant chlorogenic acid (CGA), the expression patterns of genes encoding enzymes that are associated with CGA synthesis were studied in normally developed Xuehua pear fruit. The study demonstrated that CGA content in peel and flesh of Xuehua pear decreased as fruit development progressed, with a higher level in peel. The expression levels of PbPAL1, PbPAL2, PbC3H, PbC4H, Pb4CL1, Pb4CL2, Pb4CL6, PbHCT1 and PbHCT3 genes decreased in fruit, which was consistent with the pattern of variation in CGA content. That indicated that these genes might be key genes for influencing fruit CGA synthesis in Xuehua pear. However, Pb4CL7 gene expression profile is not consistent with variation of CGA content, hence, it may not be a key gene involved in CGA synthesis.

Key words: cinnamate 4-hydroxylase gene, hydroxy cinnamoyl CoA, shikimate/quinic acid hydroxycinnamoyl transferase gene, ρ-coumarate 3´-hydroxylase gene, 4-hydroxycinnamoyl-CoA ligase gene, phenylalanine ammonia lyase gene, Xuehua pear

HE Jin-gang, CHENG Yu-dou, GUAN Jun-feng, GE Wen-ya, ZHAO Zhe. [J]. Journal of Integrative Agriculture, 2017, 16(02): 471-477.

HE Jin-gang, CHENG Yu-dou, GUAN Jun-feng, GE Wen-ya, ZHAO Zhe. Changes of chlorogenic acid content and its synthesis-associated genes expression in Xuehua pear fruit during development[J]. Journal of Integrative Agriculture, 2017, 16(02): 471-477.

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Changes of chlorogenic acid content and its synthesis-associated genes expression in Xuehua pear fruit during development

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