Expression and functional analyses of the mitogen-activated protein kinase (MPK) cascade genes in response to phytohormones in wheat (Triticum aestivum L.)

doi:10.1016/S2095-3119(16)61367-9

Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (01): 27-35.DOI: 10.1016/S2095-3119(16)61367-9

收稿日期:2015-12-28 出版日期:2017-01-20 发布日期:2017-01-08

Expression and functional analyses of the mitogen-activated protein kinase (MPK) cascade genes in response to phytohormones in wheat (Triticum aestivum L.)

YAO Su-fei^2*, WANG Yan-xia^3*, YANG Tong-ren², HAO Lin^{1, 2}, LU Wen-jing², XIAO Kai¹

¹College of Agronomy, Agricultural University of Hebei, Baoding 071001, P.R.China
² College of Life Sciences, Agricultural University of Hebei, Baoding 071001, P.R.China
³Shijiazhuang Academy of Agriculture and Forestry Sciences, Shijiazhuang 050041, P.R.China

Received:2015-12-28 Online:2017-01-20 Published:2017-01-08
Contact: XIAO Kai, Tel: +86-312-7528115, Fax: +86-312-7528400, E-mail: xiaokai@hebau.edu.cn; LU Wen-jing, Tel: +86-312-7528246, Fax: +86-312-7528200, E-mail: luwenjing@sohu.com
Supported by:
This work was financially supported by the National Natural Science Foundation of China (31371618, 31201674), the National Transgenic Major Program of China (2011ZX08008) and the Key Laboratory of Crop Growth Regulation of Hebei Province, China.

摘要/Abstract

Abstract: Mitogen-activated protein kinase (MPK) cascades consist of a set of kinase types (MPKKKs, MPKKs, MPKs) to establish
conserved signal-transducing modules mediating plant growth, development as well as responses to internal and external
cues. In this study, the expression patterns of six MPKKK, two MPKK, and 11 MPK genes in wheat in responses to external
treatments of phytohormones, including naphthylacetic acid (NAA), abscisic acid (ABA), 6-benzyladenine (6-BA), gibberellin
(GA^₃), salisylic acid (SA), jasmonic acid (JA), and ethylene (ETH), were investigated. Expression analysis revealed
that several of the MPK cascade genes are responses to the external phytohormone signaling. Of which, TaMPKKKA;3
is induced by 6-BA and NAA while TaMPK4 repressed by ETH, GA_3, SA, and JA; TaMPKKKA, TaMPKKKA;3 and TaMPK1
are down-regulated by ETH and GA^₃whereas TaMPK9 and TaMPK12 repressed by ETH and JA in addition that TaMPK12
also repressed by GA_3; TaMPK12;1 is down-regulated by ABA, GA₃ and SA while TaMPK17 repressed by all exogenous
phytonormones examined. TaMPK4, a MPK type gene previously characterized to mediate tolerance to phosphate (Pi)
deprivation, was functionally evaluated for its role in mediation of responses of plants to exogenous GA₃, ETH, SA, and JA.
Results indicated that overexpression and antisense expression of TaMPK4 in tobacco dramatically modify the growth of
seedlings upon treatments of GA₃, SA and JA, in which the overexpressors behaved deteriorated growth feature whereas
the seedlings with antisense expression of TaMPK4 exhibited improved seedling phenotype. The growth behaviors in
lines overexpressing or antisensely expressing TaMPK4 are closely associated with the biomass and the corresponding
hormone-associated parameters. These results demonstrated that TaMPK4 acts as a critical player in mediating the phytohormone
signaling. Our findings have identified the phytohormone-responsive MPK cascade genes in wheat and provided
a connection between the phytohormone-mediated responses and the MPK cascade pathways.

Key words: wheat (Triticum aestivum L.), phytohormone, mitogen-activated protein kinase (MPK) cascade, expression, transgene analysis

YAO Su-fei, WANG Yan-xia, YANG Tong-ren, HAO Lin, LU Wen-jing, XIAO Kai. [J]. Journal of Integrative Agriculture, 2017, 16(01): 27-35.

YAO Su-fei, WANG Yan-xia, YANG Tong-ren, HAO Lin, LU Wen-jing, XIAO Kai. Expression and functional analyses of the mitogen-activated protein kinase (MPK) cascade genes in response to phytohormones in wheat (Triticum aestivum L.)[J]. Journal of Integrative Agriculture, 2017, 16(01): 27-35.

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Expression and functional analyses of the mitogen-activated protein kinase (MPK) cascade genes in response to phytohormones in wheat (Triticum aestivum L.)

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