Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (9): 2023-2031.DOI: 10.1016/S2095-3119(15)61318-1

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  • 收稿日期:2015-12-07 出版日期:2016-09-02 发布日期:2016-09-02

Flavonoid content and expression analysis of flavonoid biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.) with double colors

WU Yan-qing1, WEI Meng-ran1, ZHAO Da-qiu1, 2, TAO Jun1, 2   

  1. 1 Jiangsu Key Laboratory of Crop Genetics and Physiology/College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, P.R.China
    2 Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, P.R.China
  • Received:2015-12-07 Online:2016-09-02 Published:2016-09-02
  • Contact: TAO Jun, Tel: +86-514-87997219, Fax: +86-514-87347537, E-mail: taojun@yzu.edu.cn
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (31372097 and 31400592), the Major Project of College Natural Science Research of Jiangsu Province, China (13KJA210005), the Opening Project of Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, China (2014014) and the Priority Academic Program Development from Jiangsu Government, China.

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Abstract:     Herbaceous peony (Paeonia lactiflora Pall.) is a famous flower with medicinal values, and its flowers have a number of medicinal constituents, especially flavonoids. In this study, a P. lactiflora cultivar with double colors including white outer-petal and yellow inner-petal was used as the experimental materials to perform the qualitative and quantitative analysis of flavonoids by high-performance liquid chromatograph-electrospray ionization-mass spectrometry (HPLC-ESI-MSn) and investigate the expression patterns of flavonoid biosynthetic genes using real-time quantitative polymerase chain reaction (Q-PCR). The results showed that the colors of both petals gradually weakened with flower development. Moreover, one main anthocyanin composition (peonidin 3,5-di-O-glucoside) and five main anthoxanthin compositions (kaempferol di-hexoside, kaempferol-3-O-malonylglucoside-7-O-glucoside, quercetin-3-O-galactoside, luteolin-7-O-glucoside and isorhamnetin-3-O-glucoside) were found in the both, differing significantly in their peak areas only. Total anthocyanin, anthoxanthin and flavonoid contents in white outer-petal and yellow inner-petal gradually decreased during flower development, and were consistently higher in white outer-petal. Furthermore, the expression patterns of nine structural genes in P. lactiflora flavonoid biosynthetic pathway showed that the expression levels of phenylalanine ammonialyase gene (PlPAL), chalcone synthase gene (PlCHS), flavanone 3-hydroxylase gene (PlF3H), anthocyanidin synthase gene (PlANS) and UDP-glucoside: flavonoid 5-O-glucosyltransferase gene (PlF5GT) in two petals basically presented declined tendencies, and transcription levels of PlPAL, PlCHS, PlANS, PlF3GT and PlF5GT also tended to be higher in white outer-petal, which was correlated with their flavonoid contents. These results would lay a solid foundation for the exploration and utilization of flavonoid resources in P. lactiflora flowers.

Key words: herbaceous peony ,  flower color ,  flavonoids ,  gene expression