Physiological changes and expression characteristics of ZIP family genes under zinc deficiency in navel orange (Citrus sinensis)

doi:10.1016/S2095-3119(15)61276-X

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (4): 803-811.DOI: 10.1016/S2095-3119(15)61276-X

Physiological changes and expression characteristics of ZIP family genes under zinc deficiency in navel orange (Citrus sinensis)

XING Fei^{1, 2}, FU Xing-zheng¹, WANG Nan-qi^{1, 2}, XI Jian-long¹, HUANG Yi^{1, 2}, ZHOU Wei^{1, 2}, LING Li-li¹, PENG Liang-zhi¹

¹Citrus Research Institute, Southwest University, Chongqing 400712, P.R.China
² College of Horticulture and Landscape, Southwest University, Chongqing 400715, P.R.China

收稿日期:2015-04-24 出版日期:2016-04-01 发布日期:2016-04-07
通讯作者: FU Xing-zheng, Tel: +86-23-68349725, E-mail: fuxingzheng@cric.cn; PENG Liang-zhi, Tel/Fax: +86-23-68247009, E-mail: penglz809@163.com
作者简介:XING Fei, E-mail: xingfly1218@163.com
基金资助:
This work was financially supported by the National Natural Science Foundation of China (31301742, 31201587), the Natural Science Foundation of Chongqing of China (CSTC2013JCYJA80018), the Special Fund of Chongqing Key Laboratory (CSTC), China and the Earmarked Fund of China Agricultural Research System (CARS-27-02A), China.

Physiological changes and expression characteristics of ZIP family genes under zinc deficiency in navel orange (Citrus sinensis)

XING Fei^{1, 2}, FU Xing-zheng¹, WANG Nan-qi^{1, 2}, XI Jian-long¹, HUANG Yi^{1, 2}, ZHOU Wei^{1, 2}, LING Li-li¹, PENG Liang-zhi¹

¹Citrus Research Institute, Southwest University, Chongqing 400712, P.R.China
² College of Horticulture and Landscape, Southwest University, Chongqing 400715, P.R.China

Received:2015-04-24 Online:2016-04-01 Published:2016-04-07
Contact: FU Xing-zheng, Tel: +86-23-68349725, E-mail: fuxingzheng@cric.cn; PENG Liang-zhi, Tel/Fax: +86-23-68247009, E-mail: penglz809@163.com
About author:XING Fei, E-mail: xingfly1218@163.com
Supported by:
This work was financially supported by the National Natural Science Foundation of China (31301742, 31201587), the Natural Science Foundation of Chongqing of China (CSTC2013JCYJA80018), the Special Fund of Chongqing Key Laboratory (CSTC), China and the Earmarked Fund of China Agricultural Research System (CARS-27-02A), China.

摘要/Abstract

摘要： Zinc (Zn) deficiency is widespread among citrus plants, but information about the mechanisms for Zn deficiency response in these plants is scarce. In the present study, different navel orange (Citrus sinensis (L.) Osbeck) leaves with various yellowing levels were sampled in our experimental orchard, and upon estimation of nutrient contents, Zn deficiencies were diagnosed as mild, moderate, and severe. Further analysis of chlorophyll content, photosynthetic characteristics, antioxidant enzyme activities, and expression levels of Zn/Iron-regulated transporter-like protein (ZIP) family genes were conducted in the sampled Zn-deficient leaves. The results showed that chlorophyll contents and net photosynthetic rate (P_n) seemed to decrease with reduced Zn contents. In addition, comparison of severe Zn-deficient and normal leaves revealed that activities of peroxidase (POD) and catalase (CAT) increased significantly, whereas that of Zn-containing enzymes such as Cu/Zn superoxide dismutase (Cu/Zn-SOD) significantly reduced with decreasing Zn contents. As expected, expression of the ZIP family genes, ZIP1, ZIP3, and ZIP4, was induced by Zn deficiencies. These results deepen our understanding of Zn deficiency in citrus plants as well as provide useful preliminary information for further research.

Abstract: Zinc (Zn) deficiency is widespread among citrus plants, but information about the mechanisms for Zn deficiency response in these plants is scarce. In the present study, different navel orange (Citrus sinensis (L.) Osbeck) leaves with various yellowing levels were sampled in our experimental orchard, and upon estimation of nutrient contents, Zn deficiencies were diagnosed as mild, moderate, and severe. Further analysis of chlorophyll content, photosynthetic characteristics, antioxidant enzyme activities, and expression levels of Zn/Iron-regulated transporter-like protein (ZIP) family genes were conducted in the sampled Zn-deficient leaves. The results showed that chlorophyll contents and net photosynthetic rate (P_n) seemed to decrease with reduced Zn contents. In addition, comparison of severe Zn-deficient and normal leaves revealed that activities of peroxidase (POD) and catalase (CAT) increased significantly, whereas that of Zn-containing enzymes such as Cu/Zn superoxide dismutase (Cu/Zn-SOD) significantly reduced with decreasing Zn contents. As expected, expression of the ZIP family genes, ZIP1, ZIP3, and ZIP4, was induced by Zn deficiencies. These results deepen our understanding of Zn deficiency in citrus plants as well as provide useful preliminary information for further research.

Key words: navel orange , Zn deficiency , physiological changes , ZIP family gene expression

XING Fei, FU Xing-zheng, WANG Nan-qi, XI Jian-long, HUANG Yi, ZHOU Wei, LING Li-li, PENG Liang-zhi. Physiological changes and expression characteristics of ZIP family genes under zinc deficiency in navel orange (Citrus sinensis)[J]. Journal of Integrative Agriculture, 2016, 15(4): 803-811.

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Physiological changes and expression characteristics of ZIP family genes under zinc deficiency in navel orange (Citrus sinensis)

Physiological changes and expression characteristics of ZIP family genes under zinc deficiency in navel orange (Citrus sinensis)

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