Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (2): 249-261.DOI: 10.1016/S2095-3119(15)61076-0

• 论文 • 上一篇    下一篇

Identification and characterization of the GH3 gene family in maize

 ZHANG Dong-feng, ZHANG Nan, ZHONG Tao, WANG Chao, XU Ming-liang, YE Jian-rong   

  1. National Maize Improvement Center/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.China
  • 收稿日期:2015-01-16 出版日期:2016-02-05 发布日期:2016-02-01
  • 通讯作者: YE Jian-rong, Tel: +86-10-62731135, Fax: +86-10-62733808, E-mail: yejr2006@cau.edu.cn
  • 基金资助:

    This work was financially supported by the National Natural Science Foundation of China (31371625).

Identification and characterization of the GH3 gene family in maize

 ZHANG Dong-feng, ZHANG Nan, ZHONG Tao, WANG Chao, XU Ming-liang, YE Jian-rong   

  1. National Maize Improvement Center/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.China
  • Received:2015-01-16 Online:2016-02-05 Published:2016-02-01
  • Contact: YE Jian-rong, Tel: +86-10-62731135, Fax: +86-10-62733808, E-mail: yejr2006@cau.edu.cn
  • Supported by:

    This work was financially supported by the National Natural Science Foundation of China (31371625).

摘要: The phytohormone auxin plays a central role in coordinating plant growth and development. GH3 is one of the three gene families that respond rapidly during auxin stimulation. Here, we report the identification and characterization of the GH3 gene family in maize. A total of 12 GH3 genes were identified, which are not evenly distributed over the 10 maize chromosomes. Maize GH3 protein sequences share a conserved domain which occupies nearly the entire protein. Diversified cis-elements were found in promoters of maize GH3 genes. In this study, the 12 maize GH3 proteins were primarily classified into two phylogenetic groups, similar to the 13 rice GH3 proteins, while 9 of the 19 Arabidopsis GH3 proteins were observed in the third phylogenetic group. Microarray analysis showed that expression of maize GH3 genes is temporally and spatially modulated. Additionally, maize GH3 genes displayed variable changes at transcript level upon pathogen infection. Results presented here provide insight into the diversification and evolution of GH3 proteins, and lay a foundation for the functional characterization of these GH3 genes in future, especially for elucidating the mechanisms of GH3-mediated pathogenesis.

关键词: auxin , GH3 family , pathogen infection , maize (Zea mays L.)

Abstract: The phytohormone auxin plays a central role in coordinating plant growth and development. GH3 is one of the three gene families that respond rapidly during auxin stimulation. Here, we report the identification and characterization of the GH3 gene family in maize. A total of 12 GH3 genes were identified, which are not evenly distributed over the 10 maize chromosomes. Maize GH3 protein sequences share a conserved domain which occupies nearly the entire protein. Diversified cis-elements were found in promoters of maize GH3 genes. In this study, the 12 maize GH3 proteins were primarily classified into two phylogenetic groups, similar to the 13 rice GH3 proteins, while 9 of the 19 Arabidopsis GH3 proteins were observed in the third phylogenetic group. Microarray analysis showed that expression of maize GH3 genes is temporally and spatially modulated. Additionally, maize GH3 genes displayed variable changes at transcript level upon pathogen infection. Results presented here provide insight into the diversification and evolution of GH3 proteins, and lay a foundation for the functional characterization of these GH3 genes in future, especially for elucidating the mechanisms of GH3-mediated pathogenesis.

Key words: auxin , GH3 family , pathogen infection , maize (Zea mays L.)