Accumulation characteristic of protein bodies in different regions of wheat endosperm under drought stress

doi:10.1016/S2095-3119(16)61332-1

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (12): 2921-2930.DOI: 10.1016/S2095-3119(16)61332-1

• 论文 • 上一篇

收稿日期:2015-11-20 出版日期:2016-12-01 发布日期:2016-12-02

Accumulation characteristic of protein bodies in different regions of wheat endosperm under drought stress

CHEN Xin-yu¹, LI Bo², SHAO Shan-shan¹, WANG Lei-lei¹, ZHU Xiao-wei¹, YANG yang¹, WANG Wen-jun¹, YU Xu-run¹, XIONG Fei¹

¹ Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, P.R.China
² Jiangsu Yanjiang Institute of Agricultural Sciences, Nantong 226541, P.R.China

Received:2015-11-20 Online:2016-12-01 Published:2016-12-02
Contact: XIONG Fei, Tel: +86-514-87979030, E-mail: feixiong@yzu.edu.cn
About author:CHEN Xin-yu, E-mail: 402099561@qq.com
Supported by:
This study was supported by the National Natural Science Foundation of China (31171482, 31571573), a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD), the Foundation of Excellent Doctoral Dissertation of Yangzhou University, China and the Jiangsu Province College Students Innovation Training Program in 2015, China (201511117063Y).

摘要/Abstract

Abstract: The structural characteristics of protein body accumulation in different endosperm regions of hard wheat cultivar (XM33) and soft wheat cultivar (NM13) under drought stress were investigated. Drought stress treatment was implemented from plant regreening to the caryopsis mature stage. Microscope images of endosperm cells were obtained using resin semi-thin slice technology to observe the distribution and relative area of protein body (PB). Compared with NM13, relative PB area of XM33 was significantly higher in sub-aleurone endosperm region. The amount of accumulation, including the size and relative area of PB, in two wheat cultivars was higher in sub-aleurone region than that in central region at 18 days post anthesis (DPA). Drought stress significantly enhanced the sizes and relative areas of PBs in the dorsal and abdominal endosperms in two wheat cultivars. Particularly for dorsal endosperm, drought stress enhanced the relative PB area at 18 DPA and NM13 (5.0% vs. 6.73%) showed less enhancement than XM33 (5.49% vs. 8.96%). However, NM13 (9.58% vs. 12.02%) showed greater enhancement than XM33 (10.25% vs. 11.7%) at 28 DPA. The protein content in the dorsal and abdominal endosperms of the two wheat cultivars decreased at 12 DPA and then increased until 38 DPA. Drought stress significantly increased the protein contents in the two main regions. From 12 to 38 DPA, the amount of PB accumulation and the protein content were higher in XM33 than those in NM13. The results revealed that PB distribution varied in different endosperm tissues and that the amount of PB accumulation was remarkably augmented by drought stress.

Key words: wheat , protein bodies , distribution , accumulation , drought stress

CHEN Xin-yu, LI Bo, SHAO Shan-shan, WANG Lei-lei, ZHU Xiao-wei, YANG yang, WANG Wen-jun, YU Xu-run, XIONG Fei. [J]. Journal of Integrative Agriculture, 2016, 15(12): 2921-2930.

CHEN Xin-yu, LI Bo, SHAO Shan-shan, WANG Lei-lei, ZHU Xiao-wei, YANG yang, WANG Wen-jun, YU Xu-run, XIONG Fei. Accumulation characteristic of protein bodies in different regions of wheat endosperm under drought stress[J]. Journal of Integrative Agriculture, 2016, 15(12): 2921-2930.

参考文献

Altenbach S B. 2012. New insights into the effects of high temperature, drought and post-anthesis fertilizer on wheat grain development. Journal of Cereal Science, 56, 39–50.

Barnabás B, Jäger K, Fehér A. 2008. The effect of drought and heat stress on reproductive processes in cereals. Plant Cell and Environment, 31, 11–38.

Bartel D, Thompson R D. 1986. Synthesis of messenger-RNAs coding for abundant endosperm proteins during wheat grain development. Plant Science, 46, 117–125.

Chope G A, Wan Y, Penson S P, Bhandari D G, Powers S J, Shewry P R, Hawkesford M J. 2014. Effects of genotype, season, and nitrogen nutrition on gene expression and protein accumulation in wheat grain. Journal of Agricultural and Food Chemistry, 62, 4399–4407.

Daniel C, Triboï E. 2002. Changes in wheat protein aggregation during grain development: Effects of temperatures and water stress. European Journal of Agronomy, 16, 1–12.

Dombrink-Kurtzman M A, Bietz J A. 1993. Zein composition in hard and soft endosperm of maize. Cereal Chemistry, 70, 105–108.

Farrand E A, Hinton J J C. 1974. Study of relationships between wheat protein contents of two U.K. varieties and derived flour protein contents at varying extraction rates. II. Studies by hand-dissection of individual grains. Cereal Chemistry, 51, 66–73.

Fábián A, Jäger K, Rakszegi M, Barnabás B. 2011. Embryo and endosperm development in wheat (Triticum aestivum L.) kernels subjected to drought stress. Plant Cell Reports, 30, 551–563.

Flagella Z, Giuliani M M, Giuzio L, Volpi C, Masci S. 2010. In?uence of waterde?cit on durum wheat storage protein composition and technological quality. European Journal of Agronomy, 33, 197–207.

Galili G, Altschuler Y, Levanony H, Giorini-Silfen S, Shimoni Y, Shani N, Karchi H. 1995. Assembly and transport of wheat storage proteins. Journal of Plant Physiology, 145, 626–631.

Ge P, Ma C, Wang S, Gao L, Li X, Guo G, Ma W, Yan Y. 2012. Comparative proteomic analysis of grain development in two spring wheat varieties under drought stress. Analytical and Bioanalytical Chemistry, 402, 1297–1313.

Gooding M J, Ellis R H, Shewry P R, Schofield J D. 2003. Effects of restricted water availability and increased temperature on the grain ?lling, drying and quality of winter wheat. Journal of Cereal Science, 37, 295–309.

Gupta R B, Masci S, Lafiandra D, Bariana H S, MacRitchie F. 1996. Accumulation of protein subunits and their polymers in developing grains of hexaploidwheats. Journal of Experimental Botany, 47, 1377–1385.

He Z F. 1985. Analytical Techniques for Grain Quality of Cereals and Oils. China Agriculture Press, Beijing. pp. 37–41. (in Chinese)

Jamieson P D, Martin R J, Francis G S. 1995. Drought in?uences on grain yield of barley, wheat, and maize. New Zealand Journal of Crop and Horticultural Science, 23, 55–66.

Lizana X C, Riegel R, Gomez L D, Herrera J, Isla A, Simon J, Queen M M, Calderini D F. 2010. Expansins expression is associated with grain size dynamics in wheat (Triticum aestivum). Journal of Experimental Botany, 61, 1147–1157.

Loussert C, Popineau Y, Mangavel C. 2008. Protein bodies ontogeny and localization of prolamin components in the developing endosperm of wheat caryopses. Journal of Cereal Science, 47, 445–456.

Normand F L, Hogan J T, Deobald H J. 1965. Protein content of successive peripheral layers milled from wheat, barley, grain sorghum, and glutinous rice by tangential abrasion. Cereal Chemistry, 42, 359.

Pierre C S, Peterson C J, Ross A S, Ohm J B, Verhoeven M C, Larson M, Hoefer B. 2008. Winter wheat genotypes under different levels of nitrogen and water stress: Changes in grain protein composition. Journal of Cereal Science, 47, 407–416.

Rubin R, Levanony H, Galili G. 1992. Evidence for the presence of two different types of protein bodies in wheat endosperm. Plant Physiology, 99, 718–724.

Shewry P R, Napier J A, Tatham A S. 1995. Grain storage proteins - structures and biosynthesis. The Plant Cell, 7, 945–956.

Song J, Li H, Dai S, Liu A, Cheng D, Liu J, Zhao Z. 2010. Yield component analysis of Jimai 22 with super high yield potential and wide adaption. Journal of Nuclear Agricultural Sciences, 24, 1280–1285.

Sorensen M B, Cameron-Mills V, Brandt A. 1989. Transcriptional and posttranscriptional regulation of gene expression in developing barley endosperm. Molecular & General Genetics, 217, 195–201.

Szucs A, Jäger K, Jurca M E, Fábián A, Bottka S, Zvara A, Barnabás B, Fehér A. 2010. Histological and microarray analysis of the direct effect of water shortage alone or combined with heat on early grain development in wheat (Triticum aestivum). Physiologia Plantarum, 140, 174–188.

Tosi P. 2012. Traf?cking and deposition of prolamins in wheat. Journal of Cereal Science, 56, 81–90.

Tosi P, Gritsch C S, He J, Shewry P R. 2011. Distribution of gluten proteins in bread wheat (Triticum aestivum) grain. Annals of Botany, 108, 23–35.

Ugalde T D, Jenner C F. 1990. Substrate gradients and regional patterns of dry matter deposition within developing wheat endosperm. I. Carbohydrates. Functional Plant Biology, 17, 377–394.

Wan Y, Poole R L, Huttly A K, Toscano-Underwood C, Feeney K, Welham S, Gooding M J, Mills C, Edwards K J, Shewry P R, Mitchell R A C. 2008. Transcriptome analysis of grain development in hexaploid wheat. BMC Genomics, 9, 121.

Xiong F, Yu X R, Zhang C, Zhou L, Wang Z. 2013. Effect of nitrogen on cellular material filling in ventral and dorsal region of endosperm of high quality wheat. Journal of Triticeae Crops, 33, 957–964.

Xiong F, Yu X R, Zhou L, Zhang J, Li B, Liu J, Wang F, Xiong A S. 2014. The in?uence of nitrogen on the development and accumulation of protein bodies in the developing endosperm of wheat caryopses. Molecular Biology Reports, 41, 689–695.

Yang Y B, Gao R Q, Yin Y P, Guan Y A, Zhang H W. 2007. Dynamic changes of free amino acid, protein and its components in wheat with different quality traits. Journal of Triticeae Crops, 27, 864–869.

Yu X R, Chen X Y, Zhou L, Zhang J, Yu H, Shao S S, Xiong F, Wang Z. 2015a. Structural development of wheat nutrient transfer tissues and their relationships with filial tissues development. Protoplasma, 252, 605–617.

Yu X R, Li B, Wang L L, Chen X Y, Wang W J, Gu Y J, Wang Z, Xiong F. 2016. Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat. Journal of the Science of Food and Agriculture, 96, 2746–2754.

Yu X R, Yu H, Shao S S, Zhang J, Zhou L, Zheng Y K, Xiong F, Wang Z. 2015b. Structural development of conducting cell and its functions in wheat caryopsis. Brazilian Journal of Botany, 38, 401–409.

Yu X R, Zhou L, Zhang J, Yu H, Gao D R, Zhang B Q, Xiong F, Wang Z. 2015c. Comparison of structural development and biochemical accumulation of waxy and non-waxy wheat caryopses. Cereal Research Communications, 43, 307–317.

Zhang Y F, Huang X W, Wang L L, Wei L, Wu Z H, You M S, Li B Y. 2014. Proteomic analysis of wheat seed in response to drought stress. Journal of Integrative Agriculture, 13, 919–925.

Zheng Y K, Wang Z. 2014a. Differentiation mechanism and function of the cereal aleurone cells and hormone effects on them. Plant Cell Reports, 33, 1779–1787.

Zheng Y K, Wang Z. 2014b. Protein accumulation in aleurone cells, sub-aleurone cells and the center starch endosperm of cereals. Plant Cell Reports, 33, 1607–1615.

Accumulation characteristic of protein bodies in different regions of wheat endosperm under drought stress

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