Expression analysis, single nucleotide polymorphisms within SIRT4 and SIRT7 genes and their association with body size and meat quality traits in Qinchuan cattle

doi:10.1016/S2095-3119(16)61419-3

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (12): 2819-2826.DOI: 10.1016/S2095-3119(16)61419-3

收稿日期:2016-01-15 出版日期:2016-12-01 发布日期:2016-12-02

Expression analysis, single nucleotide polymorphisms within SIRT4 and SIRT7 genes and their association with body size and meat quality traits in Qinchuan cattle

GUI Lin-sheng^1*, XIN Xiao-ling^2*, WANG Jia-li^1*, HONG Jie-yun^1*, ZAN Lin-sen¹

¹ College of Animal Science and Technology, Northwest A&F University, Yangling 712100, P.R.China
² Institute of Animal Husbandry and Veterinary Medicine, Henan Academy of Agricultural Scinece, Zhengzhou 450008, P.R.China

Received:2016-01-15 Online:2016-12-01 Published:2016-12-02
Contact: ZAN Lin-sen, Tel: +86-29-87091923, Fax: +86-29-87092164, E-mail: zanlinsen@163.com
About author:GUI Lin-sheng, E-mail: guilinsheng1234@163.com;
Supported by:
The research was supported by the National 863 Program of China (2013 AA102505), the National Natural Science Foundation of China (31272411 and 31402044), the National Beef and Yak Industrial Technology System, China (CARS-38), the China Postdoctoral Science Foundation (2016M590976), and the Key Technologies R&D Program of Henan Province, China (122102110062).

摘要/Abstract

Abstract: Silent information regulator 2 (Sir2) proteins, or sirtuins, are nicotine adenine dinucleotide (NAD)-dependent deacetylases that connect metabolism with longevity in lower organisms. In mammals, there are seven Sir2 homologs, namely, silent information regulators (SIRT1–7). SIRT4 and SIRT7 genes play a crucial role in regulating lipid metabolism, cellular growth and metabolism. This suggests that they are potential candidate genes for affecting body size and meat quality traits in animals. Hence, this study aimed to detect genetic variations of both SIRT4 and SIRT7 bovine genes in Qinchuan cattle, and to evaluate the effect of these variations on economically important body size and meat quality traits. Expression analysis using quantitative real-time PCR (qPCR) indicated that SIRT4 and SIRT7 were broadly expressed in all thirteen studied tissues. The expression of SIRT4 was higher in liver, muscle, and in subcutaneous fat tissue. In the case of SIRT7, the expression was higher in lung, abomasum, and subcutaneous fat. Using DNA sequencing, a total of three single nucleotide polymorphisms (SNPs) were identified within SIRT4 and SIRT7 genes in 468 Qinchuan cattle. These included one novel SNP within 3´ untranslated regions (UTR) of SIRT4 (SNP1: g.13915A>G) and two novel synonymous substitutions in SIRT7 (SNP2: g.3587C>T and SNP3: g.3793T>C). Statistical analyses indicated that all three SNPs could significantly influence some body size and meat quality traits in Qinchuan cattle. These novel findings will provide a background for application of bovine SIRT4 and SIRT7 genes in the selection program of Chinese cattle.

Key words: SIRT4 , SIRT7 , single nucleotide polymorphisms (SNPs) , beef cattle

GUI Lin-sheng, XIN Xiao-ling, WANG Jia-li, HONG Jie-yun, ZAN Lin-sen. [J]. Journal of Integrative Agriculture, 2016, 15(12): 2819-2826.

GUI Lin-sheng, XIN Xiao-ling, WANG Jia-li, HONG Jie-yun, ZAN Lin-sen. Expression analysis, single nucleotide polymorphisms within SIRT4 and SIRT7 genes and their association with body size and meat quality traits in Qinchuan cattle[J]. Journal of Integrative Agriculture, 2016, 15(12): 2819-2826.

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Expression analysis, single nucleotide polymorphisms within SIRT4 and SIRT7 genes and their association with body size and meat quality traits in Qinchuan cattle

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