Hot air treatment activates defense responses and induces resistance against Botrytis cinerea in strawberry fruit

doi:10.1016/S2095-3119(16)61387-4

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (11): 2658-2665.DOI: 10.1016/S2095-3119(16)61387-4

收稿日期:2015-11-06 出版日期:2016-11-04 发布日期:2016-11-04

Hot air treatment activates defense responses and induces resistance against Botrytis cinerea in strawberry fruit

JIN Peng¹, ZHENG Cong¹, HUANG Yu-ping¹, WANG Xiao-li², LUO Zi-sheng³, ZHENG Yong-hua¹

¹ College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, P.R.China
² College of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an 223003, P.R.China
³ College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, P.R.China

Received:2015-11-06 Online:2016-11-04 Published:2016-11-04
Contact: ZHENG Yong-hua, Tel: +86-25-84399080, Fax: +86-25-84395618, E-mail: zhengyh@njau.edu.cn
Supported by:
This study was supported by the Special Fund for Agro-scientific Research in the Public Interest of China (201303073), and the Fundamental Research Funds for the Central Universities, China (KYZ201420).

摘要/Abstract

Abstract: The effect of hot air (HA, 45°C, 3.5 h) treatment on reducing gray mold caused by Botrytis cinerea in strawberry fruit and the possible mechanisms were investigated. The results showed that HA treatment significantly reduced lesion diameter and enhanced activities of chitinase (CHI), β-1,3-glucanase and phenylalanine ammonia-lyase (PAL) in strawberry fruit. Total phenolic contents were also increased by HA treatment. The activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) were higher in HA treated strawberry fruit than those in control. Expression of three defense related genes such as CAT, CCR-1 allele and PLA6 was greatly induced in HA treated strawberry fruit with or without inoculation by B. cinerea. In addition, the in vitro experiment showed that HA treatment inhibited spore germination and tube growth of B. cinerea. These results suggested that HA treatment directly activated disease resistance against B. cinerea in strawberry fruit without priming response and directly inhibiting growth of B. cinerea.

Key words: strawberry , hot air , postharvest disease , induced resistance , Botrytis cinerea

JIN Peng, ZHENG Cong, HUANG Yu-ping, WANG Xiao-li, LUO Zi-sheng, ZHENG Yong-hua. [J]. Journal of Integrative Agriculture, 2016, 15(11): 2658-2665.

JIN Peng, ZHENG Cong, HUANG Yu-ping, WANG Xiao-li, LUO Zi-sheng, ZHENG Yong-hua. Hot air treatment activates defense responses and induces resistance against Botrytis cinerea in strawberry fruit[J]. Journal of Integrative Agriculture, 2016, 15(11): 2658-2665.

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Hot air treatment activates defense responses and induces resistance against Botrytis cinerea in strawberry fruit

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