Hepatitis associated with hepatitis B virus in broilers

doi:10.1016/S2095-3119(14)60914-X

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (1): 191-199.DOI: 10.1016/S2095-3119(14)60914-X

Hepatitis associated with hepatitis B virus in broilers

ZHAO Yue, MAO Jing-jing, SHE Rui-ping, HU Feng-jiao, Majid H Soomro, LIANG Rui-ping, YANG Yi-fei, DU Fang, WANG Tong-tong, GUO Zhao-jie, CHENG Min-heng

1、Laboratory of Animal Pathology and Public Health/Key Laboratory of Zoonosis, Ministry of Agriculture/College of Veterinary
Medicine, China Agricultural University, Beijing 100193, P.R.China
2、National Shanghai Center for New Drug Safety Evaluation Research Center, Shanghai 201203, P.R.China

收稿日期:2014-10-24 出版日期:2016-01-08 发布日期:2016-01-11
通讯作者: SHE Rui-ping, Tel: +86-10-62734316, Fax: +86-10-62733321,E-mail: sheruiping@126.com
作者简介:ZHAO Yue, E-mail: tsukimoon@126.com
基金资助:
This work was supported by the National Natural Science Foundation of China (31072110 and 31272515).

Hepatitis associated with hepatitis B virus in broilers

ZHAO Yue, MAO Jing-jing, SHE Rui-ping, HU Feng-jiao, Majid H Soomro, LIANG Rui-ping, YANG Yi-fei, DU Fang, WANG Tong-tong, GUO Zhao-jie, CHENG Min-heng

1、Laboratory of Animal Pathology and Public Health/Key Laboratory of Zoonosis, Ministry of Agriculture/College of Veterinary
Medicine, China Agricultural University, Beijing 100193, P.R.China
2、National Shanghai Center for New Drug Safety Evaluation Research Center, Shanghai 201203, P.R.China

Received:2014-10-24 Online:2016-01-08 Published:2016-01-11
Contact: SHE Rui-ping, Tel: +86-10-62734316, Fax: +86-10-62733321,E-mail: sheruiping@126.com
About author:ZHAO Yue, E-mail: tsukimoon@126.com
Supported by:
This work was supported by the National Natural Science Foundation of China (31072110 and 31272515).

摘要/Abstract

摘要： Infection by hepatitis B virus (HBV) results in acute and chronic liver damages in humans. Liver products of broilers as a primary food consumed in our daily life have a close connection with public health. The prevalence of the virus in livers and serum of broilers is of great significance, owning to the potential transmission between chickens and humans. Liver tissues and serum samples were tested to investigate the prevalence of hepatitis B virus infection in slaughtered broilers, for expression of HBV antigens and antibodies. The distribution and positive rate of hepatitis B surface antigen (HBsAg), hepatitis B core antigen (HBcAg) and hepatitis B e antigen (HBeAg) in liver samples were examined using immunohistochemistry. HBsAg was mainly located in the cytoplasm of hepatocytes with a positivity of 81.61% whereas HBeAg and HBcAg were primarily located in the nucleus of hepatocytes with a positivity of 40.13 and 49.10%, respectively. Enzyme-linked immunosorbent assay (ELISA) analysis of serum for HBV serological markers demonstrated a high prevalence of hepatiits B surface antibody (HBsAb, 54.91%) and hepatitis B core antibody (HBcAb, 27.68%), whereas HBeAb, HBsAg and HBeAg were rarely detectable. Classic hepatitis pathological changes, including swollen hepatocytes, focal parenchymal necrosis, lymphocytic infiltration and hyperplasia of fibrous connective tissues were observed using histopathological analysis. Some of the liver samples were found positive for HBV DNA using nested PCR. Sequence comparison confirmed that all sequences shared 97.5–99.3% identity with human HBV strains. These results demonstrated the existence of HBV in livers and serums of broilers. Animals or animal products contaminated with HBV could raise an important public health concern over food safety and zoonotic risk.

关键词: broilers , hepatitis B , virus detection , prevalence investigation

Abstract: Infection by hepatitis B virus (HBV) results in acute and chronic liver damages in humans. Liver products of broilers as a primary food consumed in our daily life have a close connection with public health. The prevalence of the virus in livers and serum of broilers is of great significance, owning to the potential transmission between chickens and humans. Liver tissues and serum samples were tested to investigate the prevalence of hepatitis B virus infection in slaughtered broilers, for expression of HBV antigens and antibodies. The distribution and positive rate of hepatitis B surface antigen (HBsAg), hepatitis B core antigen (HBcAg) and hepatitis B e antigen (HBeAg) in liver samples were examined using immunohistochemistry. HBsAg was mainly located in the cytoplasm of hepatocytes with a positivity of 81.61% whereas HBeAg and HBcAg were primarily located in the nucleus of hepatocytes with a positivity of 40.13 and 49.10%, respectively. Enzyme-linked immunosorbent assay (ELISA) analysis of serum for HBV serological markers demonstrated a high prevalence of hepatiits B surface antibody (HBsAb, 54.91%) and hepatitis B core antibody (HBcAb, 27.68%), whereas HBeAb, HBsAg and HBeAg were rarely detectable. Classic hepatitis pathological changes, including swollen hepatocytes, focal parenchymal necrosis, lymphocytic infiltration and hyperplasia of fibrous connective tissues were observed using histopathological analysis. Some of the liver samples were found positive for HBV DNA using nested PCR. Sequence comparison confirmed that all sequences shared 97.5–99.3% identity with human HBV strains. These results demonstrated the existence of HBV in livers and serums of broilers. Animals or animal products contaminated with HBV could raise an important public health concern over food safety and zoonotic risk.

Key words: broilers , hepatitis B , virus detection , prevalence investigation

ZHAO Yue, MAO Jing-jing, SHE Rui-ping, HU Feng-jiao, Majid H Soomro, LIANG Rui-ping, YANG Yi-fei, DU Fang, WANG Tong-tong, GUO Zhao-jie, CHENG Min-heng. Hepatitis associated with hepatitis B virus in broilers[J]. Journal of Integrative Agriculture, 2016, 15(1): 191-199.

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Hepatitis associated with hepatitis B virus in broilers

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