Development and evaluation of a herbal formulation with anti-pathogenic activities and probiotics stimulatory effects

doi:10.1016/S2095-3119(15)61146-7

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (05): 1103-1111.DOI: 10.1016/S2095-3119(15)61146-7

Development and evaluation of a herbal formulation with anti-pathogenic activities and probiotics stimulatory effects

ZHOU Qian^{1, 2}, WANG Si-si¹, YANG Guang¹, ZHAO Wen^{1, 2}, LI Hui-ling¹

¹ College of Food Science and Technology, Agricultural University of Hebei, Baoding 071001, P.R.China
² Engineering Technology Research Center for Agricultural Product Processing of Hebei, Baoding 071001, P.R.China

收稿日期:2015-04-24 出版日期:2016-05-03 发布日期:2016-05-03
通讯作者: ZHAO Wen, Tel: +86-312-7528426, Mobile: +86-13582820221, Fax: +86-312-7528189, E-mail: 13582820221@163.com
作者简介:ZHOU Qian, E-mail: zhouqianyz513@126.com
基金资助:
This study was supported by Hebei Science and Technology Support Program of China (13226602D). The authors are deeply grateful to the laboratory of Engineering Technology Research Center for Agricultural Product Processing of Hebei, China.

Development and evaluation of a herbal formulation with anti-pathogenic activities and probiotics stimulatory effects

ZHOU Qian^{1, 2}, WANG Si-si¹, YANG Guang¹, ZHAO Wen^{1, 2}, LI Hui-ling¹

¹ College of Food Science and Technology, Agricultural University of Hebei, Baoding 071001, P.R.China
² Engineering Technology Research Center for Agricultural Product Processing of Hebei, Baoding 071001, P.R.China

Received:2015-04-24 Online:2016-05-03 Published:2016-05-03
Contact: ZHAO Wen, Tel: +86-312-7528426, Mobile: +86-13582820221, Fax: +86-312-7528189, E-mail: 13582820221@163.com
About author:ZHOU Qian, E-mail: zhouqianyz513@126.com
Supported by:
This study was supported by Hebei Science and Technology Support Program of China (13226602D). The authors are deeply grateful to the laboratory of Engineering Technology Research Center for Agricultural Product Processing of Hebei, China.

摘要/Abstract

Abstract: Searching alternative feed antibiotics is always a study hotspot in the field of animal production. In this study, the anti-pathogenic activities and probiotics stimulatory effects of 30 kinds of herbs were screened through Oxford cup method and Hungate roll method. 15 herbs showed significantly antibacterial activities (P<0.05) against Escherichia coli, Salmonella enteritidis, Salmonella typhimurium and Staphylococcus aureus. Seven herbs showed greatly stimulatory promoting effects on Lactobacillus acidophilus and Bifidobacterium longum. Finally, five herbs were combined for the developed formulation with selective antibacterial properties and they were Mume Fructus (35%), Isatidis Folium (25%), Moslae Herba (20%), Chrysanthemi Indici Flos (13%) and Bupleuri Radix (7%). The herbal formulation showed significantly antibacterial abilities against four pathogens and stimulatory promoting abilities on two probiotics in vitro and the equivalent activities in broiler chickens in vivo against E. coli and L. acidophilus. The toxicity study showed it had no toxicity, which indicated that it would be a kind of preferred candidate for an alternative antibiotic in future animal production.

Key words: herbal formulation , alternative feed antibiotic , anti-pathogenic activity , probiotic stimulatory effect

ZHOU Qian, WANG Si-si, YANG Guang, ZHAO Wen, LI Hui-ling. Development and evaluation of a herbal formulation with anti-pathogenic activities and probiotics stimulatory effects[J]. Journal of Integrative Agriculture, 2016, 15(05): 1103-1111.

参考文献

Allen H K, Levine U Y, Looft T, Bandrick M, Casey T A. 2013. Treatment, promotion, commotion: Antibiotic alternatives in food-producing animals. Trends in Microbiology, 21, 114–119.

Andrew S M, Moyes K M, Borm A A, Fox L K, Leslie K E, Hogan J S, Oliver S P, Schukken Y H, Owens W E, Norman C. 2009. Factors associated with the risk of antibiotic residues and intramammary pathogen presence in milk from heifers administered prepartum intramammary antibiotic therapy. Veterinary Microbiology, 134, 150–156.

Burel C. 2012. Alternatives to antimicrobial growth promoters (AGPs) in animal feed. In: Fink-Gremmels J, ed. Animal feed Contamination: Effects on Livestock and Food Safety. Woodhead Publishing Ltd., Cambridge, UK. pp. 432–448.

Burt S. 2004. Essential oils: Their antibacterial properties and potential applications in foods - A review. International Journal of Food Microbiology, 94, 223–253.

Chen Y, Wong R K, Seneviratne C J, Hagg U, Mcgrath C, Samaranayake L P, Kao R. 2011. The antimicrobial efficacy of Fructus mume extract on orthodontic bracket: A monospecies-biofilm model study in vitro. Archives of Oralbiology, 56, 16–21.

China R, Mukherjee S, Sen S, Bose S, Datta S, Koley H, Ghosh S, Dhar P. 2012. Antimicrobial activity of Sesbania grandiflora flower polyphenol extracts on some pathogenic bacteria and growth stimulatory effect on the probiotic organism Lactobacillus acidophilus. Microbiological Research, 167, 500–506.

Cho J H, Kim H J, Kim I H. 2014. Effects of phytogenic feed additive on growth performance, digestibility, blood metabolites, intestinal microbiota, meat color and relative organ weight after oral challenge with Clostridium perfringens in broilers. Livestock Science, 160, 82–88.

Cushnie T, Lamb A J. 2005. Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents, 26, 343–356.

EC (European Commission). 2003. Regulation 1831 of the European Parliament and of the Council of 22 September 2003 on Additivies for Use In Animal Nutrition. Official Journal of the European Union, L268/29, Athens.

Gai Q Y, Jiao J, Mu P S, Wang W, Luo M, Li C Y, Zu Y G, Wei F Y, Fu Y J. 2013. Microwave-assisted aqueous enzymatic extraction of oil from Isatis indigotica seeds and its evaluation of physicochemical properties, fatty acid compositions and antioxidant activities. Industrial Crops and Products, 45, 303–311.

Giannenas I, Tontis D, Tsalie E, Chronis E F, Doukas D, Kyriazakis I. 2010. Influence of dietary mushroom Agaricus bisporus on intestinal morphology and microflora composition in broiler chickens. Research in Veterinary Science, 89, 78–84.

Hashemi S R, Zulkifli I, Davoodi H, Zunita Z, Ebrahimi M. 2012. Growth performance, intestinal microflora, plasma fatty acid profile in broiler chickens fed herbal plant (Euphorbia hirta) and mix of acidifiers. Animal Feed Science and Technology, 178, 167–174.

Hungate R E. 1969. A roll-tube method for cultivation of strict anaerobes. Methods Microbiol, 3B, 117–132.

Landy N, Ghalamkari G, Toghyani M. 2011. Performance, carcass characteristics, and immunity in broiler chickens fed dietary neem (Azadirachta indica) as alternative for an antibiotic growth promoter. Livestock Science, 142, 305–309.

Kalemba D, Kunicka A. 2003. Antibacterial and antifungal properties of essential oils. Current Medicinal Chemistry, 10, 813–829.

Khan M S A, Ahmad I. 2012. Biofilm inhibition by Cymbopogon citratus and Syzygium aromaticum essential oils in the strains of Candida albicans. Journal of Ethnopharmacology, 140, 416–423.

Kwon H A, Kwon Y J, Kwon D Y, Lee J H. 2008. Evaluation of antibacterial effects of a combination of Coptidis Rhizoma, Mume Fructus, and Schizandrae Fructus against Salmonella. International Journal of Food Microbiology, 127, 180–183.

Martínez J L. 2009. Environmental pollution by antibiotics and by antibiotic resistance determinants. Environmental Pollution, 157, 2893–2902.

Molan A L, Flanagan J, Wei W, Moughan P J. 2009. Selenium-containing green tea has higher antioxidant and prebiotic activities than regular green tea. Food Chemistry, 114, 820–835.

Morel A F, Araujo C A, Silva U F, Hoelzel S C S M, Záchia R, Bastos N R. 2002. Antibacterial cyclopeptide alkaloids from the bark of Condalia buxifolia. Phytochemistry, 61, 561–566.

Mountzouris K C, Paraskevas V, Tsirtsikos P, Palamidi I, Steiner T, Schatzmayr G, Fegeros K. 2011. Assessment of a phytogenic feed additive effect on broiler growth performance, nutrient digestibility and caecal microflora composition. Animal Feed Science and Technology, 168, 223–231.

NRC (National Research Council, USA). 1994. Nutrient Requirement of Poultry. 9th ed. National Academy Press, Washington, D.C.

Nuengchamnong N, Krittasilp K, Ingkaninan K. 2009. Rapid screening and identification of antioxidants in aqueous extracts of Houttuynia cordata using LC-ESI-MS coupled with DPPH assay. Food Chemistry, 117, 750–756.

Oh J, Jo H, Cho A R, Kim S J, Han J. 2013. Antioxidant and antimicrobial activities of various leafy herbal teas. Food Control, 31, 403–409.

Qu H, Zhang Y, Chai X, Sun W. 2012. Isoforsythiaside, an antioxidant and antibacterial phenylethanoid glycoside isolated from Forsythia suspensa. Bioorganic Chemistry, 40, 87–91.

Rashid M U, Weintraub A, Nord C E. 2012. Effect of new antimicrobial agents on the ecological balance of human microflora. Anaerobe, 18, 249–253.

Salminen S, Bouley C, Boutron-Rualt M C, Cummings J H, Frank A, Gibson G R, Isolauri E, Moreau M C, Roberfroid M, Rowland I. 1998. Functional food science and gastrointestinal physiology and function. British Journal of Nutrition, 1, 147–171.

Shamat M A. 1993. The role of the gastrointestinal microflora in the metabolism of drugs. International Journal of Pharmaceutics, 97, 1–13.

Shan B, Cai Y, Brooks J D, Corke H. 2007. The in vitro antibacterial activity of dietary spice and medicinal herb extracts. International Journal of Food Microbiology, 117, 112–119.

Sheng Y, Qi X, Liu Y, Guo M, Chen S, He X, Huang K, Xu W. 2014. Subchronic toxicity study in vivo and allergenicity study in vitro for genetically modi?ed rice that expresses pharmaceutical protein (human serum albumin). Food and Chemical Toxicology, 72, 242–246.

Takaishi H, Matsuki T, Nakazawa A, Takada T, Kado S, Asahara T, Kamada N, Sakuraba A, Yajima T, Higuchi H, Inoue N, Ogata H, Iwao Y, Nomoto K, Tanaka R, Hibi T. 2008. Imbalance in intestinal microflora constitution could be involved in the pathogenesis of inflammatory bowel disease. International Journal of Medical Microbiology, 298, 463–472.

Taylor N G H, Verner-Jeffreys D W, Baker-Austin C. 2011. Aquatic systems: Maintaining, mixing and mobilizing antimicrobial resistance? Trends in Ecology & Evolution, 26, 278–284.

Tian F, Li B, Ji B, Yang J, Zhang G, Chen Y, Luo Y. 2009. Antioxidant and antimicrobial activities of consecutive extracts from Galla chinensis: The polarity affects the bioactivities. Food Chemistry, 113, 173–179.

Vimal S K, Sharma D, Bhatnagar M. 2010. Anti-bacterial activity of herbal extracts, EuMiL and antibiotics against Helicobacter muridarum. Pharmacognosy, 2, 436–441.

Wen L F, He J G. 2012. Dose-response effects of an antimicrobial peptide, a cecropin hybrid, on growth performance, nutrient utilisation, bacterial counts in the digesta and intestinal morphology in broilers. British Journal of Nutrition, 108, 1756–1763.

Williamson E M. 2001. Synergy and other interaction in phytomedicines. Phytomedicine, 8, 401–409.

Wyllie E, Wyllie R, 1991. Routine laboratory monitoring for serious adverse effects of antiepileptic medications: The controversy. Epilepsia, 32(Suppl. 5), 74–79.

Ye C L, Dai D H, Hu W L. 2013. Antimicrobial and antioxidant activities of the essential oil from onion (Allium cepa L.). Food Control, 30, 48–53.

Ye M, Liu D, Zhang R, Yang L, Wang J. 2012. Effect of hawk tea (Litsea coreana L.) on the numbers of lactic acid bacteria and flavour compounds of yoghurt. International Dairy Journal, 23, 68–71.

Yoo S, Murata R M, Duarte S. 2011. Antimicrobial traits of tea- and cranberry derived polyphenols against Streptococcus mutans. Caries Research, 45, 327–335.

Zhang G, Hu M, He L, Fu P, Wang L, Zhou J. 2013. Optimization of microwave-assisted enzymatic extraction of polyphenols from waste peanut shells and evaluation of its antioxidant and antibacterial activities in vitro. Food and Bioproducts Processing, 91, 158–168.

Zhu S, Yang Y, Yu H, Ying Y, Zou G. 2005. Chemical composition and antimicrobial activity of the essential oils of Chrysanthemum indicum. Journal of Ethnopharmacology, 96, 151–158.

Development and evaluation of a herbal formulation with anti-pathogenic activities and probiotics stimulatory effects

Development and evaluation of a herbal formulation with anti-pathogenic activities and probiotics stimulatory effects

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