Mapping resistant QTLs for rice sheath blight disease with a doubled haploid population

doi:10.1016/S2095-3119(14)60909-6

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (5): 801-810.DOI: 10.1016/S2095-3119(14)60909-6

• 论文 • 下一篇

Mapping resistant QTLs for rice sheath blight disease with a doubled haploid population

ZENG Yu-xiang, XIA Ling-zhi, WEN Zhi-hua, JI Zhi-juan, ZENG Da-li, QIAN Qian, YANG Chang-deng

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.China

收稿日期:2014-05-16 出版日期:2015-05-01 发布日期:2015-05-13
通讯作者: YANG Chang-deng, Tel/Fax: +86-571-63370367, E-mail: yangchangdeng@126.com; QIAN Qian,Tel/Fax: +86-571-63371418, E-mail: qianqian188@hotmail.com
作者简介:* These authors contributed equally to this study.
基金资助:
This work was supported by the National Natural Science Foundation of China (31101004, 31221004), the National High-Tech R&D Program of China (863 Program, 2012AA101201), a fund from Zhejiang Province for public welfare (2014C32013), a special fund for technical innovation team in Zhejiang Province, China (2010R50024), and a fund from the Chinese Academy of Agricultural Sciences to the Scientific and Technical Innovation Team.

Mapping resistant QTLs for rice sheath blight disease with a doubled haploid population

ZENG Yu-xiang, XIA Ling-zhi, WEN Zhi-hua, JI Zhi-juan, ZENG Da-li, QIAN Qian, YANG Chang-deng

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.China

Received:2014-05-16 Online:2015-05-01 Published:2015-05-13
Contact: YANG Chang-deng, Tel/Fax: +86-571-63370367, E-mail: yangchangdeng@126.com; QIAN Qian,Tel/Fax: +86-571-63371418, E-mail: qianqian188@hotmail.com
About author:* These authors contributed equally to this study.
Supported by:
This work was supported by the National Natural Science Foundation of China (31101004, 31221004), the National High-Tech R&D Program of China (863 Program, 2012AA101201), a fund from Zhejiang Province for public welfare (2014C32013), a special fund for technical innovation team in Zhejiang Province, China (2010R50024), and a fund from the Chinese Academy of Agricultural Sciences to the Scientific and Technical Innovation Team.

摘要/Abstract

摘要： Sheath blight (SB) disease, caused by Rhizoctonia solani Kühn, is one of the most serious diseases causing rice (Oryza sativa L.) yield loss worldwide. A doubled haploid (DH) population was constructed from a cross between a japonica variety CJ06 and an indica variety TN1, and to analyze the quantitative trait loci (QTLs) for SB resistance under three different environments (environments 1–3). Two traits were recorded to evaluate the SB resistance, namely lesion height (LH) and disease rating (DR). Based on field evaluation of SB resistance and a genetic map constructed with 214 markers, a total of eight QTLs were identified for LH and eight QTLs for DR under three environments, respectively. The QTLs for LH were anchored on chromosomes 1, 3, 4, 5, 6, and 8, and explained 4.35–17.53% of the phenotypic variation. The SB resistance allele of qHNLH4 from TN1 decreased LH by 3.08 cm, and contributed to 17.53% of the variation at environment 1. The QTL for LH (qHZaLH8) detected on chromosome 8 in environment 2 explained 16.71% of the variation, and the resistance allele from CJ06 reduced LH by 4.4 cm. Eight QTLs for DR were identified on chromosomes 1, 5, 6, 8, 9, 11, and 12 under three conditions with the explained variation from 2.0 to 11.27%. The QTL for DR (qHZaDR8), which explained variation of 11.27%, was located in the same interval as that of qHZaLH8, both QTLs were detected in environment 2. A total of six pairs of digenic epistatic loci for DR were detected in three conditions, but no epistatic locus was observed for LH. In addition, we detected 12 QTLs for plant height (PH) in three environments. None of the PH-QTLs were co-located with the SB-QTLs. The results facilitate our understanding of the genetic basis for SB resistance in rice.

关键词: rice , sheath blight , quantitative trait locus , resistance

Abstract: Sheath blight (SB) disease, caused by Rhizoctonia solani Kühn, is one of the most serious diseases causing rice (Oryza sativa L.) yield loss worldwide. A doubled haploid (DH) population was constructed from a cross between a japonica variety CJ06 and an indica variety TN1, and to analyze the quantitative trait loci (QTLs) for SB resistance under three different environments (environments 1–3). Two traits were recorded to evaluate the SB resistance, namely lesion height (LH) and disease rating (DR). Based on field evaluation of SB resistance and a genetic map constructed with 214 markers, a total of eight QTLs were identified for LH and eight QTLs for DR under three environments, respectively. The QTLs for LH were anchored on chromosomes 1, 3, 4, 5, 6, and 8, and explained 4.35–17.53% of the phenotypic variation. The SB resistance allele of qHNLH4 from TN1 decreased LH by 3.08 cm, and contributed to 17.53% of the variation at environment 1. The QTL for LH (qHZaLH8) detected on chromosome 8 in environment 2 explained 16.71% of the variation, and the resistance allele from CJ06 reduced LH by 4.4 cm. Eight QTLs for DR were identified on chromosomes 1, 5, 6, 8, 9, 11, and 12 under three conditions with the explained variation from 2.0 to 11.27%. The QTL for DR (qHZaDR8), which explained variation of 11.27%, was located in the same interval as that of qHZaLH8, both QTLs were detected in environment 2. A total of six pairs of digenic epistatic loci for DR were detected in three conditions, but no epistatic locus was observed for LH. In addition, we detected 12 QTLs for plant height (PH) in three environments. None of the PH-QTLs were co-located with the SB-QTLs. The results facilitate our understanding of the genetic basis for SB resistance in rice.

Key words: rice , sheath blight , quantitative trait locus , resistance

ZENG Yu-xiang, XIA Ling-zhi, WEN Zhi-hua, JI Zhi-juan, ZENG Da-li, QIAN Qian, YANG Chang-deng. Mapping resistant QTLs for rice sheath blight disease with a doubled haploid population[J]. Journal of Integrative Agriculture, 2015, 14(5): 801-810.

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Mapping resistant QTLs for rice sheath blight disease with a doubled haploid population

Mapping resistant QTLs for rice sheath blight disease with a doubled haploid population

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