Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (4): 603-609.DOI: 10.1016/S2095-3119(14)60774-7

• 论文 •    下一篇

Comparative genetic mapping revealed powdery mildew resistance gene MlWE4 derived from wild emmer is located in same genomic region of Pm36 and Ml3D232 on chromosome 5BL

 ZHANG Dong, OUYANG Shu-hong, WANG Li-li, CUI Yu, WU Qiu-hong, LIANG Yong, WANG Zhen-zhong, XIE Jing-zhong, ZHANG De-yun, WANG Yong, CHEN Yong-xing, LIU Zhi-yong   

  1. State Key Laboratory for Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, P.R.China
  • 收稿日期:2014-02-26 出版日期:2015-04-01 发布日期:2015-04-10
  • 通讯作者: LIU Zhi-yong, Tel: +86-10-62731211,E-mail: zhiyongliu@cau.edu.cn
  • 基金资助:

    This work was financially supported by the National High- Tech R&D Program of China (2011AA100104), the National Basic Research Program of China (2013CB127705), the National Natural Science Foundation of China (31030056, 31210103902), and the Introducing Talents of Disciplines to Universities, Ministry of Education (MOE) of China (111-02-3).

Comparative genetic mapping revealed powdery mildew resistance gene MlWE4 derived from wild emmer is located in same genomic region of Pm36 and Ml3D232 on chromosome 5BL

 ZHANG Dong, OUYANG Shu-hong, WANG Li-li, CUI Yu, WU Qiu-hong, LIANG Yong, WANG Zhen-zhong, XIE Jing-zhong, ZHANG De-yun, WANG Yong, CHEN Yong-xing, LIU Zhi-yong   

  1. State Key Laboratory for Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, P.R.China
  • Received:2014-02-26 Online:2015-04-01 Published:2015-04-10
  • Contact: LIU Zhi-yong, Tel: +86-10-62731211,E-mail: zhiyongliu@cau.edu.cn
  • Supported by:

    This work was financially supported by the National High- Tech R&D Program of China (2011AA100104), the National Basic Research Program of China (2013CB127705), the National Natural Science Foundation of China (31030056, 31210103902), and the Introducing Talents of Disciplines to Universities, Ministry of Education (MOE) of China (111-02-3).

摘要: Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most devastating wheat diseases. Wild emmer wheat (Triticum turgidum ssp. dicoccoides) is a promising source of disease resistance for wheat. A powdery mildew resistance gene conferring resistance to B. graminis f. sp. tritici isolate E09, originating from wild emmer wheat, has been transferred into the hexaploid wheat line WE4 through crossing and backcrossing. Genetic analyses indicated that the powdery mildew resistance was controlled by a single dominant gene, temporarily designated MlWE4. By mean of comparative genomics and bulked segregant analysis, a genetic linkage map of MlWE4 was constructed, and MlWE4 was mapped on the distal region of chromosome arm 5BL. Comparative genetic linkage maps showed that genes MlWE4, Pm36 and Ml3D232 were co-segregated with markers XBD37670 and XBD37680, indicating they are likely the same gene or alleles in the same locus. The co-segregated markers provide a starting point for chromosome landing and map-based cloning of MlWE4, Pm36 and Ml3D232.

关键词: wild emmer , powdery mildew resistance gene , Pm36 , comparative genomics

Abstract: Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most devastating wheat diseases. Wild emmer wheat (Triticum turgidum ssp. dicoccoides) is a promising source of disease resistance for wheat. A powdery mildew resistance gene conferring resistance to B. graminis f. sp. tritici isolate E09, originating from wild emmer wheat, has been transferred into the hexaploid wheat line WE4 through crossing and backcrossing. Genetic analyses indicated that the powdery mildew resistance was controlled by a single dominant gene, temporarily designated MlWE4. By mean of comparative genomics and bulked segregant analysis, a genetic linkage map of MlWE4 was constructed, and MlWE4 was mapped on the distal region of chromosome arm 5BL. Comparative genetic linkage maps showed that genes MlWE4, Pm36 and Ml3D232 were co-segregated with markers XBD37670 and XBD37680, indicating they are likely the same gene or alleles in the same locus. The co-segregated markers provide a starting point for chromosome landing and map-based cloning of MlWE4, Pm36 and Ml3D232.

Key words: wild emmer , powdery mildew resistance gene , Pm36 , comparative genomics