Effect of graded levels of fiber from alfalfa meal on apparent and standardized ileal digestibility of amino acids of growing pigs

doi:10.1016/S2095-3119(14)60924-2

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (12): 2598-2604.DOI: 10.1016/S2095-3119(14)60924-2

Effect of graded levels of fiber from alfalfa meal on apparent and standardized ileal digestibility of amino acids of growing pigs

CHEN Liang, GAO Li-xiang, LIU Li, DING Ze-ming, ZHANG Hong-fu

1、State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2、Key Laboratory for Feed Biotechnology, Ministry of Agriculture/Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

收稿日期:2014-10-10 出版日期:2015-12-01 发布日期:2015-12-16
通讯作者: ZHANG Hong-fu, Tel/Fax: +86-10-62818910,E-mail: zhanghfcaas@gmail.com
作者简介:CHEN Liang, E-mail: shengji0202@126.com;These authors contributed equally to this study.
基金资助:
This work was financially supported by the Project of State Key Laboratory of Animal Nutrition, Ministry of Science and Technology, China (2004DA125184G1104) and the Agricultural Science and Technology Innovation Program, Chinese Academy of Agricultural Sciences, China (ASTIP-IAS07).

Effect of graded levels of fiber from alfalfa meal on apparent and standardized ileal digestibility of amino acids of growing pigs

CHEN Liang, GAO Li-xiang, LIU Li, DING Ze-ming, ZHANG Hong-fu

1、State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2、Key Laboratory for Feed Biotechnology, Ministry of Agriculture/Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2014-10-10 Online:2015-12-01 Published:2015-12-16
Contact: ZHANG Hong-fu, Tel/Fax: +86-10-62818910,E-mail: zhanghfcaas@gmail.com
About author:CHEN Liang, E-mail: shengji0202@126.com;These authors contributed equally to this study.
Supported by:
This work was financially supported by the Project of State Key Laboratory of Animal Nutrition, Ministry of Science and Technology, China (2004DA125184G1104) and the Agricultural Science and Technology Innovation Program, Chinese Academy of Agricultural Sciences, China (ASTIP-IAS07).

摘要/Abstract

摘要： Two experiments were conducted to determine the effects of fiber level from alfalfa meal and sampling time on the apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acids (AA) in growing pigs. A total of 24 ileal-cannulated pigs (Duroc×(Large White×Landrace) with body weight (21.4±1.5) kg) were randomly allotted to 4 treatments. The pigs were provided a corn-soybean meal diet or a diet containing 5, 10 or 20% of alfalfa meal during two 10-d experimental periods. The AID of AA was measured. Six ileal-cannulated pigs were fed a protein-free diet in order to estimate the endogenous protein losses and SID of AA. Ileal AA digestibility was not affected by inclusion of 5 or 10% alfalfa meal in the diet (P>0.05). The AID of His, Lys, Met, Phe, Thr, Val, Ala, Asp, Cys, Gly, Pro, Ser and Tyr reduced by 2.0–6.8% with the addition level of alfalfa meal (linear, P<0.05). The SID of His, Lys, Ser, Thr and Tyr decreased by 2.2–4.3% as the level of alfalfa meal in the diet increased (linear, P<0.05). The AID and SID of AA were not affected by the sampling time (P>0.05). A multiple linear regression analysis, taking into account both the soluble and insoluble fiber content in the diets, explained more than 36% of variation in SID of Ser and Thr (P<0.05). In conclusion, the corn-soybean meal diet containing 10% of alfalfa meal did not affect ileal AA digestion. The AID and SID of AA were similar between two sampling times. Increasing the concentration of total dietary fiber from 12.3 to 21.4% by adding graded levels of alfalfa meal (0–20%) to a corn-soybean meal control diet induced a linear reduction in AID and SID of most AA. Soluble and insoluble fibers from alfalfa meal have differential roles in the AA digestion, which may help explain the variation observed in the SID of partial AA. These findings would provide important information for dietary fiber level and composition related to AA digestion.

关键词: alfalfa , amino acid , dietary fiber , digestibility , pig

Abstract: Two experiments were conducted to determine the effects of fiber level from alfalfa meal and sampling time on the apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acids (AA) in growing pigs. A total of 24 ileal-cannulated pigs (Duroc×(Large White×Landrace) with body weight (21.4±1.5) kg) were randomly allotted to 4 treatments. The pigs were provided a corn-soybean meal diet or a diet containing 5, 10 or 20% of alfalfa meal during two 10-d experimental periods. The AID of AA was measured. Six ileal-cannulated pigs were fed a protein-free diet in order to estimate the endogenous protein losses and SID of AA. Ileal AA digestibility was not affected by inclusion of 5 or 10% alfalfa meal in the diet (P>0.05). The AID of His, Lys, Met, Phe, Thr, Val, Ala, Asp, Cys, Gly, Pro, Ser and Tyr reduced by 2.0–6.8% with the addition level of alfalfa meal (linear, P<0.05). The SID of His, Lys, Ser, Thr and Tyr decreased by 2.2–4.3% as the level of alfalfa meal in the diet increased (linear, P<0.05). The AID and SID of AA were not affected by the sampling time (P>0.05). A multiple linear regression analysis, taking into account both the soluble and insoluble fiber content in the diets, explained more than 36% of variation in SID of Ser and Thr (P<0.05). In conclusion, the corn-soybean meal diet containing 10% of alfalfa meal did not affect ileal AA digestion. The AID and SID of AA were similar between two sampling times. Increasing the concentration of total dietary fiber from 12.3 to 21.4% by adding graded levels of alfalfa meal (0–20%) to a corn-soybean meal control diet induced a linear reduction in AID and SID of most AA. Soluble and insoluble fibers from alfalfa meal have differential roles in the AA digestion, which may help explain the variation observed in the SID of partial AA. These findings would provide important information for dietary fiber level and composition related to AA digestion.

Key words: alfalfa , amino acid , dietary fiber , digestibility , pig

CHEN Liang, GAO Li-xiang, LIU Li, DING Ze-ming, ZHANG Hong-fu. Effect of graded levels of fiber from alfalfa meal on apparent and standardized ileal digestibility of amino acids of growing pigs[J]. Journal of Integrative Agriculture, 2015, 14(12): 2598-2604.

参考文献

AOAC (Association of Official Analytical Chemists). 2007.Official Methods of Analysis. 18th ed. Association of OfficialAnalytical Chemists, Gaithersburg, MD.

Bach Knudsen K E. 1997. Carbohydrate and lignin contents ofplant materials used in animal feeding. Animal Feed Scienceand Technology, 67, 319-338

Bach Knudsen K E, Hedemann M S, Laerke H N. 2012. Therole of carbohydrates in intestinal health of pigs. AnimalFeed Science and Technology, 173, 41-53

Buraczewska L, ?wi?ch E, Tu?nio A, Taciak M, CeregrzynM, Korczyński W. 2007. The effect of pectin in amino aciddigestibility and digesta viscosity, motility and morphologyof the small intestine, and on N-balance and performanceof young pigs. Livestock Science, 109, 53-56

Castillo M, Martin-Orue S M, Anguita M, Perez J F, Gasa J.2007. Adaptation of gut microbiota to corn physical structureand different types of dietary fibre. Livestock Science, 109,149-152

Chen L, Gao L X, Zhang H F. 2014. Effect of graded levelsof fiber from alfalfa meal on nutrient digestibility and flowof fattening pigs. Journal of Integrative Agriculture, 13,1746-1752

Chen L, Zhang H F, Gao L X, Zhao F, Lu Q P, Sa R N. 2013.Effect of graded levels of fiber from alfalfa meal on intestinalnutrient flow and hindgut fermentation of growing pigs.Journal of Animal Science, 91, 4757-4764

Dégen L, Halas V, Babinszky L. 2007. Effect of dietary fibre onprotein and fat digestibility and its consequences on dietformulation for growing and fattening pigs: A review. ActaAgriculturae Scandinavica (Section A - Animal Science),57, 1-9

Dilger R N, Sands J S, Ragland D, Adeola O. 2004. Digestibilityof nitrogen and amino acids in soybean meal with addedsoyhulls. Journal of Animal Science, 82, 715-724

Le Goff G, van Milgen J, Noblet J. 2002. Influence of dietary fibreon digestive utilization and rate of passage in growing pigs,finishing pigs, and adult sows. Animal Science, 74, 503-515

Grala W, Verstegen M W A, Jansman A J M, Huisman J,Wasilewko J. 1998. Nitrogen utilization in pigs fed diets withsoybean and rapeseed products leading to different ilealendogenous nitrogen losses. Journal of Animal Science,76, 569-577

Guillon F, Saulnier L, Robert P, Thibault J F, Champ M. 2007.Chemical structure and function of cell wall through cerealgrains and vegetable samples. In: Salovaara H, GatesF, Tenkanen M, eds., Dietary Fibre Components andFunctions. Wageningen Academic Publishers, Wageningen,the Netherlands. pp. 31-64

Hooda S, Metzler-Zebeli B U, Vasanthan T, Zijlstra R T. 2011.Effects of viscosity and fermentability of dietary fibre onnutrient digestibility and digesta characteristics in ilealcannulatedgrower pigs. British Journal of Nutrition, 106,664-674

Hopwood D E, Pethick D W, Pluske J R, Hampson D J. 2004.Addition of pearl barley to a rice-based diet for newlyweaned piglets increases the viscosity of the intestinalcontents, reduces starch digestibility and exacerbates post-weaning colibacillosis. British Journal of Nutrition,92, 419-427

Jenkins D J, Kendall C W, Axelsen M, Augustin L S, VuksanV. 2000. Viscous and nonviscous fibres, nonabsorbableand low glycaemic index carbohydrates, blood lipids andcoronary heart disease. Current Opinion in Lipidology, 11,49-56

Johnson L J, Noll S, Renteria A, Shurson J. 2003. Feedingby-product high in concentration of fiber to non-ruminants.In: Third National Symposium on Alternative Feeds forLivestock and Poultry Held. Kansas City, MO, USA.

Kass M L, Van Soest P J, Pond W G, Lewis B, McDowell RE. 1980. Utilization of dietary fiber from alfalfa by growingswine. I. Apparent digestibility of diet components in specificsegments of the gastrointestinal tract. Journal of AnimalScience, 50, 175-191

de Lange C F M, Pluske J, Gong J, Nyachoti C M. 2010.Strategic use of feed ingredients and feed additives tostimulate gut health and development in young pigs.Livestock Science, 134, 124-134

Lenis N P, Bikker P, van der Meulen J, van Diepen J T M,Bakker J G M, Jongbloed A W. 1996. Effect of dietaryneutral detergent fiber on ileal digestibility and portal fluxof nitrogen and amino acids and on nitrogen utilization ingrowing pigs. Journal of Animal Science, 74, 2687-2699

Lindberg J E, Cortova Z. 1995. The effect of increasing inclusionof lucerne leaf meal in a barley-based diet on the partitionof digestion and on nutrient utilization in pigs. Animal FeedScience and Technology, 56, 11-20

Lindberg J E. 2014. Fiber effects in nutrition and gut health inpigs. Journal of Animal Science and Biotechnology, 5, 15.Longland A C, Low A G, Quelch D B, Bray S P. 1993. Adaptationto the digestion of non-starch polysaccharide in growing pigsfed on cereal or semi-purified basal diets. British Journal ofNutrition, 70, 557-566

Mosenthin R, Sauer W C, Ahrens F. 1994. Dietary pectin’seffect on ileal and fecal amino acid digestibility and exocrinepancreatic secretions in growing pigs. Journal of Nutrition,124, 1222-1229

Noblet J, Le Goff G I. 2001. Effect of dietary fibre on theenergy value of feeds for pigs. Animal Feed Science andTechnology, 90, 35-52

NRC (National Research Council, USA). 1998. NutrientRequirements of Swine. 10th ed. National Academic Press,Washington, D.C.

NRC (National Research Council, USA). 2012. NutrientRequirements of Swine. 11th ed. National Academic Press,Washington, D.C.

Panetta D M, Powers W J, Xin H, Kerr B J, Stadler K J. 2006.Nitrogen excretion and ammonia emissions from pigsfed modified diets. Journal of Environmental Quality, 35,1297-1308

Prosky L, Asp N G, Schweizer T F, DeVires J W, Fruda I. 1988.Determination of insoluble, soluble, and total dietary fiberin foods and food products: Interlaboratory study. Journal-Association of Official Analytical Chemists, 71, 1012-1023

Régnier C, Jaguelin Y, Noblet J, Renaudeau D. 2012. Ilealdigestibility of amino acids of cassava, sweet potato,cocoyam and erythrina foliages fed to growing pigs. Animal,6, 586-593

SAS (Statistical Analysis System). 2009. SAS User’s Guide:Statistics. SAS Institute, Cary, NC.

Schulze H, van Leeuwen P, Verstegen M W, Huisman J,Souffrant W B, Ahrens F. 1994. Effect of level of dietaryneutral detergent fiber on ileal apparent digestibility andileal nitrogen losses in pigs. Journal of Animal Science,72, 2362-2368

Schürch A F, Lloyd L E, Champton E W. 1950. The use ofchromic oxide as an index for determining the digestibilityof a diet. Journal of Nutrition, 50, 629-636

Souffrant W B. 2001. Effect of dietary fibre on ileal digestibilityand endogenous nitrogen losses in the pigs. Animal FeedScience and Technology, 90, 93-102

Stein H H, Gibson M L, Pedersen C, Boersma M G. 2006. Aminoacid and energy digestibility in ten samples of distillers driedgrain with solubles fed to growing pigs. Journal of AnimalScience, 84, 853-860

Stein H H, Seve B, Fuller M F, Moughan P J, De Lange CM G. 2007. Invited review: Amino acid bioavailabilityand digestibility in pig feed ingredients: Terminology andapplication. Journal Animal of Science, 85, 172-180

Thacker P A, Haq I. 2008. Nutrient digestibility, performance andcarcass traits of growing-finishing pigs fed diets containinggraded levels of dehydrated lucerne meal. Journal of theScience of Food and Agriculture, 88, 2019-2025

Urriola P E, Stein H H. 2010. Effects of distillers dried grains withsolubles on amino acid, energy, and fiber digestibility andon hindgut fermentation of dietary fiber in a corn-soybeanmeal diet fed to growing pigs. Journal of Animal Science,88, 1454-1462

Wenk C. 2001. The role of dietary fibre in the digestivephysiology of the pig. Animal Feed Science and Technology,90, 21-33

Wilfart A, Montagne L, Simmins P H, Noblet J, van Milgen J.2007. Effect of fibre content in the diet on the mean retentiontime in different segments of the digestive tract in growingpigs. Livestock Science, 109, 27-29

Yen J T. 2004. Dehydrated alfalfa meal reduces urinary ureaexcretion in finishing gilts. Journal of Animal Science,82(suppl. 2), 68.

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Effect of graded levels of fiber from alfalfa meal on apparent and standardized ileal digestibility of amino acids of growing pigs

Effect of graded levels of fiber from alfalfa meal on apparent and standardized ileal digestibility of amino acids of growing pigs

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