Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (10): 2002-2010.DOI: 10.1016/S2095-3119(14)60979-5

• 论文 • 上一篇    下一篇

High genetic variation and recombination events in the vicinity of non-autonomous transposable elements from ‘Candidatus Liberibacter asiaticus’

 WANG  Xue-feng, CHEN  Jiao-yue, TAN  Jin , DUAN  Suo, DENG  Xiao-ling, CHEN  Jian-chi, ZHOU  Chang-yong   

  1. 1、National Citrus Engineering Research Center, Citrus Research Institute, Chinese Academy of Agricultural Sciences/Southwest
    University, Chongqing 400712, P.R.China
    2、College of Plant Protection, Southwest University, Chongqing 400715, P.R.China
    3、Citrus Huanglongbing Research Center, South China Agricultural University, Guangzhou 510642, P.R.China
    4、San Joaquin Valley Agricultural Sciences Center (SJVASC), USDA-ARS, Parlier, CA 93648, USA
  • 收稿日期:2014-10-28 出版日期:2015-10-09 发布日期:2015-10-12
  • 通讯作者: ZHOU Chang-yong, Tel: +86-23-68349601, E-mail: zhoucy@cric.cn
  • 作者简介:WANG Xue-feng, E-mail: wangxuefeng@cric.cn; CHEN Jiaoyue,E-mail: chenjiaoyueok@sina.com;* These authors contributed equally to this study.
  • 基金资助:

    Funding for this work was provided by the Special Fund for Agro-Scientific Research in the Public Interest, China (201003067-02), the Natural Science Foundation Project of CQ CSTC (cstc2012jjA80025) and the Fundamental Research Funds for the Central Universities, China (XDJK2014A001, XDJK2014D004).

High genetic variation and recombination events in the vicinity of non-autonomous transposable elements from ‘Candidatus Liberibacter asiaticus’

 WANG  Xue-feng, CHEN  Jiao-yue, TAN  Jin , DUAN  Suo, DENG  Xiao-ling, CHEN  Jian-chi, ZHOU  Chang-yong   

  1. 1、National Citrus Engineering Research Center, Citrus Research Institute, Chinese Academy of Agricultural Sciences/Southwest
    University, Chongqing 400712, P.R.China
    2、College of Plant Protection, Southwest University, Chongqing 400715, P.R.China
    3、Citrus Huanglongbing Research Center, South China Agricultural University, Guangzhou 510642, P.R.China
    4、San Joaquin Valley Agricultural Sciences Center (SJVASC), USDA-ARS, Parlier, CA 93648, USA
  • Received:2014-10-28 Online:2015-10-09 Published:2015-10-12
  • Contact: ZHOU Chang-yong, Tel: +86-23-68349601, E-mail: zhoucy@cric.cn
  • About author:WANG Xue-feng, E-mail: wangxuefeng@cric.cn; CHEN Jiaoyue,E-mail: chenjiaoyueok@sina.com;* These authors contributed equally to this study.
  • Supported by:

    Funding for this work was provided by the Special Fund for Agro-Scientific Research in the Public Interest, China (201003067-02), the Natural Science Foundation Project of CQ CSTC (cstc2012jjA80025) and the Fundamental Research Funds for the Central Universities, China (XDJK2014A001, XDJK2014D004).

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摘要： Two miniature inverted-repeat transposable elements (MITEs), MCLas-A and MCLas-B, were recently identified from ‘Candidatus Liberibacter asiaticus’ known to be associated with citrus Huanglongbing (HLB, yellow shoot disease). MCLas-A was suggested as an active MITE because of its mobility. The immediate upstream gene of the two MITEs was predicted to be a putative transposase. The goal of this study is to analyze the sequence variation in the upstream putative transposase of MITEs and explore the possible correlation between sequence variation of transposase gene and MITE activity. PCR and sequence analysis showed that 12 sequence types were found in six major amplicon types from 43 representative ‘Ca. L. asiaticus’ isolates from China, the United States and Brazil. Out of the 12 sequence types, three (T4, T5-2, T6) were reported for the first time. Recombination events were found in the two unique sequence types (T5-2 and T6) which were detected in all Brazilian isolates. Notably, no sequence variation or recombination events were detected in the upstream putative transposase gene of MCLas-A, suggesting the conservation of the transposase gene might be closely related with the MITE activity. Phylogenetic analysis demonstrated two well supported clades including five subclades were identified, clearly reflecting the geographical origins of isolates, especially that of Ruili isolates, São Paulo isolates and a few Florida isolates.

关键词: Huanglongbing , ‘Candidatus Liberibacter asiaticus&rsquo, miniature inverted-repeat transposable element

Abstract: Two miniature inverted-repeat transposable elements (MITEs), MCLas-A and MCLas-B, were recently identified from ‘Candidatus Liberibacter asiaticus’ known to be associated with citrus Huanglongbing (HLB, yellow shoot disease). MCLas-A was suggested as an active MITE because of its mobility. The immediate upstream gene of the two MITEs was predicted to be a putative transposase. The goal of this study is to analyze the sequence variation in the upstream putative transposase of MITEs and explore the possible correlation between sequence variation of transposase gene and MITE activity. PCR and sequence analysis showed that 12 sequence types were found in six major amplicon types from 43 representative ‘Ca. L. asiaticus’ isolates from China, the United States and Brazil. Out of the 12 sequence types, three (T4, T5-2, T6) were reported for the first time. Recombination events were found in the two unique sequence types (T5-2 and T6) which were detected in all Brazilian isolates. Notably, no sequence variation or recombination events were detected in the upstream putative transposase gene of MCLas-A, suggesting the conservation of the transposase gene might be closely related with the MITE activity. Phylogenetic analysis demonstrated two well supported clades including five subclades were identified, clearly reflecting the geographical origins of isolates, especially that of Ruili isolates, São Paulo isolates and a few Florida isolates.

Key words: Huanglongbing , ‘Candidatus Liberibacter asiaticus&rsquo, miniature inverted-repeat transposable element