Study on the Mitochondrial Genome of Sea Island Cotton (Gossypium barbadense) by BAC Library Screening

doi:10.1016/S2095-3119(13)60595-X

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (5): 945-953.DOI: 10.1016/S2095-3119(13)60595-X

Study on the Mitochondrial Genome of Sea Island Cotton (Gossypium barbadense) by BAC Library Screening

SU Ai-guo, LI Shuang-shuang, LIU Guo-zheng, LEI Bin-bin, KANG Ding-ming, LI Zhao-hu, MA

1、Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement/College of
Agronomy and Biotechnology, China Agricultural University, Beijing 100193, P.R.China
2、College of Agronomy, Hebei Agricultural University, Baoding 071001, P.R.China

收稿日期:2013-03-08 出版日期:2014-05-01 发布日期:2014-05-06
通讯作者: HUA Jin-ping, Mobile: 13141333576, Fax: +86-10-62734748, E-mail: jinping_hua@hotmail.com
作者简介:SU Ai-guo, E-mail: sx_201.su@163.com
基金资助:
This work was supported by the Key Project Founded by Ministry of Education, China (107012) and the National Natural Science Foundation of China (31171591).

Study on the Mitochondrial Genome of Sea Island Cotton (Gossypium barbadense) by BAC Library Screening

SU Ai-guo, LI Shuang-shuang, LIU Guo-zheng, LEI Bin-bin, KANG Ding-ming, LI Zhao-hu, MA

1、Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement/College of
Agronomy and Biotechnology, China Agricultural University, Beijing 100193, P.R.China
2、College of Agronomy, Hebei Agricultural University, Baoding 071001, P.R.China

Received:2013-03-08 Online:2014-05-01 Published:2014-05-06
Contact: HUA Jin-ping, Mobile: 13141333576, Fax: +86-10-62734748, E-mail: jinping_hua@hotmail.com
About author:SU Ai-guo, E-mail: sx_201.su@163.com
Supported by:
This work was supported by the Key Project Founded by Ministry of Education, China (107012) and the National Natural Science Foundation of China (31171591).

摘要/Abstract

摘要： The plant mitochondrial genome displays complex features, particularly in terms of cytoplasmic male sterility (CMS). Therefore, research on the cotton mitochondrial genome may provide important information for analyzing genome evolution and exploring the molecular mechanism of CMS. In this paper, we present a preliminary study on the mitochondrial genome of sea island cotton (Gossypium barbadense) based on positive clones from the bacterial artificial chromosome (BAC) library. Thirty-five primers designed with the conserved sequences of functional genes and exons of mitochondria were used to screen positive clones in the genome library of the sea island cotton variety called Pima 90-53. Ten BAC clones were obtained and verified for further study. A contig was obtained based on six overlapping clones and subsequently laid out primarily on the mitochondrial genome. One BAC clone, clone 6 harbored with the inserter of approximate 115 kb mtDNA sequence, in which more than 10 primers fragments could be amplified, was sequenced and assembled using the Solexa strategy. Fifteen mitochondrial functional genes were revealed in clone 6 by gene annotation. The characteristics of the syntenic gene/exon of the sequences and RNA editing were preliminarily predicted.

关键词: Gossypium barbadense , BAC library , mitochondrial genome , RNA editing

Abstract: The plant mitochondrial genome displays complex features, particularly in terms of cytoplasmic male sterility (CMS). Therefore, research on the cotton mitochondrial genome may provide important information for analyzing genome evolution and exploring the molecular mechanism of CMS. In this paper, we present a preliminary study on the mitochondrial genome of sea island cotton (Gossypium barbadense) based on positive clones from the bacterial artificial chromosome (BAC) library. Thirty-five primers designed with the conserved sequences of functional genes and exons of mitochondria were used to screen positive clones in the genome library of the sea island cotton variety called Pima 90-53. Ten BAC clones were obtained and verified for further study. A contig was obtained based on six overlapping clones and subsequently laid out primarily on the mitochondrial genome. One BAC clone, clone 6 harbored with the inserter of approximate 115 kb mtDNA sequence, in which more than 10 primers fragments could be amplified, was sequenced and assembled using the Solexa strategy. Fifteen mitochondrial functional genes were revealed in clone 6 by gene annotation. The characteristics of the syntenic gene/exon of the sequences and RNA editing were preliminarily predicted.

Key words: Gossypium barbadense , BAC library , mitochondrial genome , RNA editing

SU Ai-guo, LI Shuang-shuang, LIU Guo-zheng, LEI Bin-bin, KANG Ding-ming, LI Zhao-hu, MA. Study on the Mitochondrial Genome of Sea Island Cotton (Gossypium barbadense) by BAC Library Screening[J]. Journal of Integrative Agriculture, 2014, 13(5): 945-953.

SU Ai-guo, LI Shuang-shuang, LIU Guo-zheng, LEI Bin-bin, KANG Ding-ming, LI Zhao-hu, MA . Study on the Mitochondrial Genome of Sea Island Cotton (Gossypium barbadense) by BAC Library Screening[J]. Journal of Integrative Agriculture, 2014, 13(5): 945-953.

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Study on the Mitochondrial Genome of Sea Island Cotton (Gossypium barbadense) by BAC Library Screening

Study on the Mitochondrial Genome of Sea Island Cotton (Gossypium barbadense) by BAC Library Screening

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