Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (11): 2452-2459.DOI: 10.1016/S2095-3119(14)60748-6

• 论文 • 上一篇    下一篇

Biochemical Mechanism of Chlorantraniliprole Resistance in the Diamondback Moth, Plutella xylostella Linnaeus

 HU Zhen-di, FENG Xia, LIN Qing-sheng, CHEN Huan-yu, LI Zhen-yu, YIN Fei, LIANG　Pei , GAO Xi-wu   

  1. 1、Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R.China
    2、Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, P.R.China
    3、Department of Entomology, China Agricultural University, Beijing 100193, P.R.China
  • 收稿日期:2013-08-02 出版日期:2014-11-06 发布日期:2014-11-14
  • 通讯作者: GAO Xi-wu, Tel: +86-10-62732974, E-mail: gaoxiwu@263.net.cn; FENG Xia, Tel: +86-20-87597577, E-mail: fengx@gdppri.com
  • 作者简介:HU Zhen-di, Tel: +86-20-87597577, E-mail: littleblackfox@126.com
  • 基金资助:

    This project was sponsored by the Special Fund for Agro- Scientific Research in the Public Interest of China (201103021), the President Foundation of Guangdong Academy of Agricultural Sciences, China (201206) and the Guangdong Natural Science Foundation, China (S2013010012529).

Biochemical Mechanism of Chlorantraniliprole Resistance in the Diamondback Moth, Plutella xylostella Linnaeus

 HU Zhen-di, FENG Xia, LIN Qing-sheng, CHEN Huan-yu, LI Zhen-yu, YIN Fei, LIANG　Pei , GAO Xi-wu   

  1. 1、Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R.China
    2、Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, P.R.China
    3、Department of Entomology, China Agricultural University, Beijing 100193, P.R.China
  • Received:2013-08-02 Online:2014-11-06 Published:2014-11-14
  • Contact: GAO Xi-wu, Tel: +86-10-62732974, E-mail: gaoxiwu@263.net.cn; FENG Xia, Tel: +86-20-87597577, E-mail: fengx@gdppri.com
  • About author:HU Zhen-di, Tel: +86-20-87597577, E-mail: littleblackfox@126.com
  • Supported by:

    This project was sponsored by the Special Fund for Agro- Scientific Research in the Public Interest of China (201103021), the President Foundation of Guangdong Academy of Agricultural Sciences, China (201206) and the Guangdong Natural Science Foundation, China (S2013010012529).

PDF

摘要： The insecticide chlorantraniliprole exhibits good efficacy and plays an important role in controlling the diamondback moth, Plutella xylostella Linnaeus. However, resistance to chlorantraniliprole has been observed recently in some field populations. At present study, diamondback moths with resistance to chlorantraniliprole (resistant ratio (RR) was 82.18) for biochemical assays were selected. The assays were performed to determine potential resistance mechanisms. The results showed that the selected resistant moths (GDLZ-R) and susceptible moth could be synergized by known metabolic inhibitors such as piperonyl butoxide (PBO), triphenyl phosphate (TPP) and diethyl-maleate (DEM) at different levels (1.68-5.50-fold and 2.20-2.89-fold, respectively), and DEM showed the maximum synergism in both strains. In enzymes assays, a high level of glutathione-S-transferase (GST) was observed in the resistant moth, in contrast, moths that are susceptible to the insecticide had only 1/3 the GST activity of the resistant moths. The analysis of short-term exposure of chlorantraniliprole on biochemical response in the resistant strain also showed that GST activity was significantly elevated after exposure to a sub-lethal concentration of chlorantraniliprole (about 1/3 LC50, 12 mg L-1) 12 and 24 h, respectively. The results show that there is a strong correlation between the enzyme activity and resistance, and GST is likely the main detoxification mechanism responsible for resistance to chlorantraniliprole in P. xylostella L., cytochrome P450 monooxygenase (P450) and carboxy-lesterase (CarE) are involved in to some extent.

关键词: Plutella xylostella , chlorantraniliprole , resistance , biochemical mechanism

Abstract: The insecticide chlorantraniliprole exhibits good efficacy and plays an important role in controlling the diamondback moth, Plutella xylostella Linnaeus. However, resistance to chlorantraniliprole has been observed recently in some field populations. At present study, diamondback moths with resistance to chlorantraniliprole (resistant ratio (RR) was 82.18) for biochemical assays were selected. The assays were performed to determine potential resistance mechanisms. The results showed that the selected resistant moths (GDLZ-R) and susceptible moth could be synergized by known metabolic inhibitors such as piperonyl butoxide (PBO), triphenyl phosphate (TPP) and diethyl-maleate (DEM) at different levels (1.68-5.50-fold and 2.20-2.89-fold, respectively), and DEM showed the maximum synergism in both strains. In enzymes assays, a high level of glutathione-S-transferase (GST) was observed in the resistant moth, in contrast, moths that are susceptible to the insecticide had only 1/3 the GST activity of the resistant moths. The analysis of short-term exposure of chlorantraniliprole on biochemical response in the resistant strain also showed that GST activity was significantly elevated after exposure to a sub-lethal concentration of chlorantraniliprole (about 1/3 LC50, 12 mg L-1) 12 and 24 h, respectively. The results show that there is a strong correlation between the enzyme activity and resistance, and GST is likely the main detoxification mechanism responsible for resistance to chlorantraniliprole in P. xylostella L., cytochrome P450 monooxygenase (P450) and carboxy-lesterase (CarE) are involved in to some extent.

Key words: Plutella xylostella , chlorantraniliprole , resistance , biochemical mechanism