Effect of Swim-Up and Percoll Treatment on Sperm Quality and In vitro Embryo Development in Yak

doi:10.1016/S2095-3119(13)60378-0

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (12): 2235-2242.DOI: 10.1016/S2095-3119(13)60378-0

Effect of Swim-Up and Percoll Treatment on Sperm Quality and In vitro Embryo Development in Yak

LIU Ben, CUI Yan , YU Si-jiu

1.Academic of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, P.R.China
2.Technology and Research Center of Gansu Province for Embryonic Engineering of Bovine and Sheep & Goat, Lanzhou 730070, P.R.China

收稿日期:2012-09-04 出版日期:2013-12-01 发布日期:2013-12-17
通讯作者: YU Si-jiu, Tel: +86-931-7631220, Fax: +86-931-7631220, E-mail: sjyu@163.com
作者简介:LIU Ben, E-mail: liubenres@gmail.com
基金资助:
This work was supported by the National Natural Science Foundation of China (30871903) and the Fundamental Research Funds for the Gansu Universities, China (2011).

Effect of Swim-Up and Percoll Treatment on Sperm Quality and In vitro Embryo Development in Yak

LIU Ben, CUI Yan , YU Si-jiu

1.Academic of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, P.R.China
2.Technology and Research Center of Gansu Province for Embryonic Engineering of Bovine and Sheep & Goat, Lanzhou 730070, P.R.China

Received:2012-09-04 Online:2013-12-01 Published:2013-12-17
Contact: YU Si-jiu, Tel: +86-931-7631220, Fax: +86-931-7631220, E-mail: sjyu@163.com
Supported by:
This work was supported by the National Natural Science Foundation of China (30871903) and the Fundamental Research Funds for the Gansu Universities, China (2011).

摘要/Abstract

摘要： This study was designed to determine the effect of different sperm preparation treatments on yak sperm quality and in vitro embryo development. Frozen-thawed semen samples were treated using swim-up or percoll gradient centrifugation methods. Sperm concentration, progressive motility, recovery of motile sperm, membrane integrity, acrosome and chromatin integrity were scored and compared in recovered samples and controls. In addition, the effects of two sperm separation treatments on embryos capable of cleavage and in vitro development to the blastocyst stage were evaluated. Swim-up separated sperm showed a higher motility, while the concentration of spermatozoa recovered and percent recovery of motile sperm were higher with percoll gradient centrifugation separation. According to the optical and electron microscopies, swim-up produced higher percentage of sperm with intact plasma membrane and acrosome than percoll gradient centrifugation separation. However, there was no difference in the percentage of sperm with intact chromatin between two treatment groups. Cell numbers in the blastocysts of two groups were not different. The blastocyst rate was similar in both groups, whereas cleavage rate was higher when swim-up was used.

关键词: yak , swim-up , percoll gradient centrifugation , embryo development

Abstract: This study was designed to determine the effect of different sperm preparation treatments on yak sperm quality and in vitro embryo development. Frozen-thawed semen samples were treated using swim-up or percoll gradient centrifugation methods. Sperm concentration, progressive motility, recovery of motile sperm, membrane integrity, acrosome and chromatin integrity were scored and compared in recovered samples and controls. In addition, the effects of two sperm separation treatments on embryos capable of cleavage and in vitro development to the blastocyst stage were evaluated. Swim-up separated sperm showed a higher motility, while the concentration of spermatozoa recovered and percent recovery of motile sperm were higher with percoll gradient centrifugation separation. According to the optical and electron microscopies, swim-up produced higher percentage of sperm with intact plasma membrane and acrosome than percoll gradient centrifugation separation. However, there was no difference in the percentage of sperm with intact chromatin between two treatment groups. Cell numbers in the blastocysts of two groups were not different. The blastocyst rate was similar in both groups, whereas cleavage rate was higher when swim-up was used.

Key words: yak , swim-up , percoll gradient centrifugation , embryo development

LIU Ben, CUI Yan , YU Si-jiu. Effect of Swim-Up and Percoll Treatment on Sperm Quality and In vitro Embryo Development in Yak[J]. Journal of Integrative Agriculture, 2013, 12(12): 2235-2242.

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Effect of Swim-Up and Percoll Treatment on Sperm Quality and In vitro Embryo Development in Yak

Effect of Swim-Up and Percoll Treatment on Sperm Quality and In vitro Embryo Development in Yak

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