The Finding and Phylogenetic Evolution Analysis of Bovine Piroplasms in the Ras?n Area of North Korea

doi:10.1016/S2095-3119(13)60377-9

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (10): 1847-1854.DOI: 10.1016/S2095-3119(13)60377-9

The Finding and Phylogenetic Evolution Analysis of Bovine Piroplasms in the Ras?n Area of North Korea

JIA Li-jun, ZHANG Shou-fa, CAO Shi-nuo, QIAN Nian-chao, YU Long-zheng , XUAN Xue-nan

1 Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Yanbian University, Yanji 133002, P.R.China
2 National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan

收稿日期:2012-04-08 出版日期:2013-10-01 发布日期:2013-10-01
通讯作者: Correspondence ZHANG Shou-fa, E-mail: shoufazhang@sina.com
作者简介:JIA Li-jun, Mobile: 13844705029, E-mail: ljjia@ybu.edu.cn
基金资助:
National Natural Science Foundation of China (30560112, 30960278), and Natural Science Foundation of Jilin Province of China (201115230).

The Finding and Phylogenetic Evolution Analysis of Bovine Piroplasms in the Ras?n Area of North Korea

JIA Li-jun, ZHANG Shou-fa, CAO Shi-nuo, QIAN Nian-chao, YU Long-zheng , XUAN Xue-nan

1 Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Yanbian University, Yanji 133002, P.R.China
2 National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan

Received:2012-04-08 Online:2013-10-01 Published:2013-10-01
Contact: Correspondence ZHANG Shou-fa, E-mail: shoufazhang@sina.com
About author:JIA Li-jun, Mobile: 13844705029, E-mail: ljjia@ybu.edu.cn
Supported by:
National Natural Science Foundation of China (30560112, 30960278), and Natural Science Foundation of Jilin Province of China (201115230).

摘要/Abstract

摘要： The objective of this study was to investigate the epidemiology of bovine Piroplasms infections in the Ras?n area of North Korea. The survey was carried out by light microscopic examination of Giemsa-stained blood smears, PCR, and phylogenetic evolution analysis of 128 blood samples collected from the Ras?n area. The results showed that the infection rates of the small and large parasites were about 2.5 and 1.5% on average, respectively, in all Theileria sergenti and Babesia ovatapositive blood smears by microscopic examination of blood smears. The detection rate of T. sergenti Giemsa-stained smears was 43.75%, while that with PCR was 67.97%. The detection rate of B. ovata Giemsa-stained smears was 49.21%, while that with PCR was 71.88%. The sequence and phylogenetic analysis of DNA showed 98.84% homology between the 18S rRNA gene sequences of T. sergenti isolates from North Korean and that of Yanbian state from China, indicating the closest genetic relationship between both of them. Moreover, 100% homology was shown between the 18S rRNA gene sequence of B. ovata isolates from North Korea and the published sequence AY081192 of GenBank, indicating the closest genetic relationship between both of them. This survey confirmed that Ras?n is the endemic area of T. sergenti and B. ovata in North Korea.

关键词: Ras?n area of North Korea , bovine Piroplasms , phylogenetic trees

Abstract: The objective of this study was to investigate the epidemiology of bovine Piroplasms infections in the Ras?n area of North Korea. The survey was carried out by light microscopic examination of Giemsa-stained blood smears, PCR, and phylogenetic evolution analysis of 128 blood samples collected from the Ras?n area. The results showed that the infection rates of the small and large parasites were about 2.5 and 1.5% on average, respectively, in all Theileria sergenti and Babesia ovatapositive blood smears by microscopic examination of blood smears. The detection rate of T. sergenti Giemsa-stained smears was 43.75%, while that with PCR was 67.97%. The detection rate of B. ovata Giemsa-stained smears was 49.21%, while that with PCR was 71.88%. The sequence and phylogenetic analysis of DNA showed 98.84% homology between the 18S rRNA gene sequences of T. sergenti isolates from North Korean and that of Yanbian state from China, indicating the closest genetic relationship between both of them. Moreover, 100% homology was shown between the 18S rRNA gene sequence of B. ovata isolates from North Korea and the published sequence AY081192 of GenBank, indicating the closest genetic relationship between both of them. This survey confirmed that Ras?n is the endemic area of T. sergenti and B. ovata in North Korea.

Key words: Ras?n area of North Korea , bovine Piroplasms , phylogenetic trees

JIA Li-jun, ZHANG Shou-fa, CAO Shi-nuo, QIAN Nian-chao, YU Long-zheng , XUAN Xue-nan. The Finding and Phylogenetic Evolution Analysis of Bovine Piroplasms in the Ras?n Area of North Korea[J]. Journal of Integrative Agriculture, 2013, 12(10): 1847-1854.

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The Finding and Phylogenetic Evolution Analysis of Bovine Piroplasms in the Ras?n Area of North Korea

The Finding and Phylogenetic Evolution Analysis of Bovine Piroplasms in the Ras?n Area of North Korea

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