The Defined siRNAs Suppress Nanog and Sox2 Expressions in Mouse ES Cells

doi:10.1016/S1671-2927(11)60141-7

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (9): 1475-1481.DOI: 10.1016/S1671-2927(11)60141-7

The Defined siRNAs Suppress Nanog and Sox2 Expressions in Mouse ES Cells

LEI Lei, DOU Lin , WANG Hua-yan

College of Veterinary Medicine/Shaanxi Center for Stem Cell Engineering and Technology, Northwest A&F University,

收稿日期:2010-08-19 出版日期:2011-09-01 发布日期:2011-09-09
通讯作者: Correspondence WANG Hua-yan, Professor, Tel: +86-29-87080069, Fax: 86-29-87080068,E-mail: hhwang101@163.com
作者简介:LEI Lei, Ph D, Tel: +86-29-87080069, E-mail: friendly0722@126.com
基金资助:
This work was supported by the National Natural ScienceFoundation of China (30871786) and the NationalBasic Research Program of China (973 Program, 2009CB941002).

The Defined siRNAs Suppress Nanog and Sox2 Expressions in Mouse ES Cells

LEI Lei, DOU Lin , WANG Hua-yan

College of Veterinary Medicine/Shaanxi Center for Stem Cell Engineering and Technology, Northwest A&F University,

Received:2010-08-19 Online:2011-09-01 Published:2011-09-09
Contact: Correspondence WANG Hua-yan, Professor, Tel: +86-29-87080069, Fax: 86-29-87080068,E-mail: hhwang101@163.com
About author:LEI Lei, Ph D, Tel: +86-29-87080069, E-mail: friendly0722@126.com
Supported by:
This work was supported by the National Natural ScienceFoundation of China (30871786) and the NationalBasic Research Program of China (973 Program, 2009CB941002).

摘要/Abstract

摘要： Nanog, Oct4 and Sox2 are important transcription factors that are expressed in embryonic stem (ES) cells or embryoniccarcinoma (EC) cells, but in most cases they are absent in somatic cells. These factors play a key role to maintain embryonic stemcell self-renew and pluripotency. Down-regulation of Nanog and Sox2 gene expression can change multiple gene expressionpatterns and signal transduction pathways, and will initiate ES cell differentiation. This study was designed to select theefficient small interfering RNA (siRNA) fragments that inhibit Nanog and Sox2 gene expression in mouse J1 ES cells and P19 ECcells. Among synthesized siRNAs we screened out the siRNA N301 for Nanog and siRNA S720 for Sox2, which not only downregulatedof Nanog and Sox2 gene expression, but also interfered embryoid bodies formation. Our study provided the definedsiRNA fragments that could be used to investigate the epigenetic function of Nanog and Sox2 genes.

关键词: Nanog, Sox2, siRNA, embryonic stem cells, embryoid body

Abstract: Nanog, Oct4 and Sox2 are important transcription factors that are expressed in embryonic stem (ES) cells or embryoniccarcinoma (EC) cells, but in most cases they are absent in somatic cells. These factors play a key role to maintain embryonic stemcell self-renew and pluripotency. Down-regulation of Nanog and Sox2 gene expression can change multiple gene expressionpatterns and signal transduction pathways, and will initiate ES cell differentiation. This study was designed to select theefficient small interfering RNA (siRNA) fragments that inhibit Nanog and Sox2 gene expression in mouse J1 ES cells and P19 ECcells. Among synthesized siRNAs we screened out the siRNA N301 for Nanog and siRNA S720 for Sox2, which not only downregulatedof Nanog and Sox2 gene expression, but also interfered embryoid bodies formation. Our study provided the definedsiRNA fragments that could be used to investigate the epigenetic function of Nanog and Sox2 genes.

Key words: Nanog, Sox2, siRNA, embryonic stem cells, embryoid body

LEI Lei, DOU Lin , WANG Hua-yan. The Defined siRNAs Suppress Nanog and Sox2 Expressions in Mouse ES Cells[J]. Journal of Integrative Agriculture, 2011, 10(9): 1475-1481.

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The Defined siRNAs Suppress Nanog and Sox2 Expressions in Mouse ES Cells

The Defined siRNAs Suppress Nanog and Sox2 Expressions in Mouse ES Cells

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