Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum

doi:10.1016/S1671-2927(11)60185-5abiotic stress| CDPK| Nicotiana tabacum| RACE| real-time qRT-PCR

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (12): 1851-1860.DOI: 10.1016/S1671-2927(11)60185-5abiotic stress| CDPK| Nicotiana tabacum| RACE| real-time qRT-PCR

Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum

CHEN Shuai, LIU Guan-shan, WANG Yuan-ying, SUN Yu-he , CHEN Jia

1. Key Laboratory of Genetic Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences,Qingdao 266101, P.R.China
2. State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, P.R.China

收稿日期:2010-07-15 出版日期:2011-12-01 发布日期:2011-12-19
通讯作者: Correspondence LIU Guan-shan, Tel: +86-532-88703168, Fax: +86-532-88703168, E-mail: liuguanshan2002@163.com
基金资助:
This study was supported in part by the Special Grand Science and Technology Projects for China National Tobacco Corporation (110200701022, 110200902036), China and the open subject from the State Key Laboratory of Plant Physiology and Biochemistry (PPB08004).

Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum

CHEN Shuai, LIU Guan-shan, WANG Yuan-ying, SUN Yu-he , CHEN Jia

1. Key Laboratory of Genetic Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences,Qingdao 266101, P.R.China
2. State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, P.R.China

Received:2010-07-15 Online:2011-12-01 Published:2011-12-19
Contact: Correspondence LIU Guan-shan, Tel: +86-532-88703168, Fax: +86-532-88703168, E-mail: liuguanshan2002@163.com
Supported by:
This study was supported in part by the Special Grand Science and Technology Projects for China National Tobacco Corporation (110200701022, 110200902036), China and the open subject from the State Key Laboratory of Plant Physiology and Biochemistry (PPB08004).

摘要/Abstract

摘要： Calcium-dependent protein kinases (CDPKs, EC 2.7.1.37) comprise a large family of Ser/Thr kinases in plants and play an important role in plant Ca2+ signal transduction. A full-length CDPK gene, NtCDPK12 (GenBank accession number GQ337420), was isolated from common tobacco (Nicotiana tabacum) leaves by rapid amplification of cDNA ends (RACE). The NtCDPK12 cDNA is 1 816 bp length and contains an open reading frame (ORF) of 1 461 bp encoding 486 amino acids. Sequence alignments indicated that NtCDPK12 contains all conserved regions found in CDPKs and shows a high level of sequence similarity to many other plant CDPKs. The results of real-time quantitative reverse transcription-PCR (qRTPCR) showed that NtCDPK12 was highly expressed in stems and increased in roots treated with high-salt or subjected to drought stress, which indicates that NtCDPK12 was induced by high-salt and drought stresses.

Abstract: Calcium-dependent protein kinases (CDPKs, EC 2.7.1.37) comprise a large family of Ser/Thr kinases in plants and play an important role in plant Ca2+ signal transduction. A full-length CDPK gene, NtCDPK12 (GenBank accession number GQ337420), was isolated from common tobacco (Nicotiana tabacum) leaves by rapid amplification of cDNA ends (RACE). The NtCDPK12 cDNA is 1 816 bp length and contains an open reading frame (ORF) of 1 461 bp encoding 486 amino acids. Sequence alignments indicated that NtCDPK12 contains all conserved regions found in CDPKs and shows a high level of sequence similarity to many other plant CDPKs. The results of real-time quantitative reverse transcription-PCR (qRTPCR) showed that NtCDPK12 was highly expressed in stems and increased in roots treated with high-salt or subjected to drought stress, which indicates that NtCDPK12 was induced by high-salt and drought stresses.

Key words: abiotic stress, CDPK, Nicotiana tabacum, RACE, real-time qRT-PCR

CHEN Shuai, LIU Guan-shan, WANG Yuan-ying, SUN Yu-he , CHEN Jia. Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum[J]. Journal of Integrative Agriculture, 2011, 10(12): 1851-1860.

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Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum

Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum

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