Fine Mapping and Cloning of the Grain Number Per-Panicle Gene (Gnp4) on Chromosome 4 in Rice (Oryza sativa L.)

doi:10.1016/S1671-2927(11)60182-X

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (12): 1825-1833.DOI: 10.1016/S1671-2927(11)60182-X

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Fine Mapping and Cloning of the Grain Number Per-Panicle Gene (Gnp4) on Chromosome 4 in Rice (Oryza sativa L.)

ZHANG Zhan-ying, LI Jin-jie, YAO Guo-xin, ZHANG Hong-liang, DOU Hui-jing, SHI Hong-li, SUN Xingming, LI Zi-chao

1.Key Laboratory of Crop Heterosis and Utilization, Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.China

收稿日期:2011-09-02 出版日期:2011-12-01 发布日期:2011-12-19
通讯作者: Correspondence LI Zi-chao, Tel: +86-10-62731414, E-mail: lizichao@cau.edu.cn
基金资助:
This work was funded by the Project of the 973 Program (2010CB125904), the 948 Program (2011-G2B), the National Natural Science Foundation of China (31171521) and the National Key Technology R&D Program of China (2009BADA2B01).

Fine Mapping and Cloning of the Grain Number Per-Panicle Gene (Gnp4) on Chromosome 4 in Rice (Oryza sativa L.)

ZHANG Zhan-ying, LI Jin-jie, YAO Guo-xin, ZHANG Hong-liang, DOU Hui-jing, SHI Hong-li, SUN Xingming, LI Zi-chao

1.Key Laboratory of Crop Heterosis and Utilization, Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.China

Received:2011-09-02 Online:2011-12-01 Published:2011-12-19
Contact: Correspondence LI Zi-chao, Tel: +86-10-62731414, E-mail: lizichao@cau.edu.cn
Supported by:
This work was funded by the Project of the 973 Program (2010CB125904), the 948 Program (2011-G2B), the National Natural Science Foundation of China (31171521) and the National Key Technology R&D Program of China (2009BADA2B01).

摘要/Abstract

摘要： Grain number per-panicle is one of the most important components for rice yield. Spikelets on the primary and secondary branches determine the grain number per-panicle in rice. In this study, we identified a natural mutant, gnp4, lack of lateral spikelet on the secondary branches in the field condition. In addition, the Gnp4 and Lax1-1 double mutant showed dramatically reduced secondary branches and spikelets in panicle at reproductive stage, and tillers at vegetative stage. By map-based cloning approach, and using four F2 segregating populations, the Gnp4 gene was finally mapped to a 10.7-kb region on the long arm of chromosome 4 in rice. In this region, only one gene was predicted, and genomic DNA sequencing of the 10.7-kb region showed no nucleotide differences between the mutant and wild type. Interestingly, we found that the methylation level of several cytosines in the promoter CpG islands region of the predicted gene in gnp4 were different from the wild type. Thus, we propose that the DNA methylation changes at these sites may induce to decrease expression level of Gnp4, consequently, resulting in phenotypic variation.

关键词: rice, gnp4, lax1-1, grain number per-panicle, methylation

Abstract: Grain number per-panicle is one of the most important components for rice yield. Spikelets on the primary and secondary branches determine the grain number per-panicle in rice. In this study, we identified a natural mutant, gnp4, lack of lateral spikelet on the secondary branches in the field condition. In addition, the Gnp4 and Lax1-1 double mutant showed dramatically reduced secondary branches and spikelets in panicle at reproductive stage, and tillers at vegetative stage. By map-based cloning approach, and using four F2 segregating populations, the Gnp4 gene was finally mapped to a 10.7-kb region on the long arm of chromosome 4 in rice. In this region, only one gene was predicted, and genomic DNA sequencing of the 10.7-kb region showed no nucleotide differences between the mutant and wild type. Interestingly, we found that the methylation level of several cytosines in the promoter CpG islands region of the predicted gene in gnp4 were different from the wild type. Thus, we propose that the DNA methylation changes at these sites may induce to decrease expression level of Gnp4, consequently, resulting in phenotypic variation.

Key words: rice, gnp4, lax1-1, grain number per-panicle, methylation

ZHANG Zhan-ying, LI Jin-jie, YAO Guo-xin, ZHANG Hong-liang, DOU Hui-jing, SHI Hong-li, SUN Xingming, LI Zi-chao. Fine Mapping and Cloning of the Grain Number Per-Panicle Gene (Gnp4) on Chromosome 4 in Rice (Oryza sativa L.)[J]. Journal of Integrative Agriculture, 2011, 10(12): 1825-1833.

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Fine Mapping and Cloning of the Grain Number Per-Panicle Gene (Gnp4) on Chromosome 4 in Rice (Oryza sativa L.)

Fine Mapping and Cloning of the Grain Number Per-Panicle Gene (Gnp4) on Chromosome 4 in Rice (Oryza sativa L.)

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