Transformation of Cabbage (Brassica oleracea L. var. capitata) with Bt cry1Ba3 Gene for Control of Diamondback Moth

doi:10.1016/S1671-2927(11)60167-3

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (11): 1693-1700.DOI: 10.1016/S1671-2927(11)60167-3

Transformation of Cabbage (Brassica oleracea L. var. capitata) with Bt cry1Ba3 Gene for Control of Diamondback Moth

YI Deng-xia, CUI Lei, LIU Yu-mei, ZHUANG Mu, ZHANG Yang-yong, FANG Zhi-yuan , YANG Li-mei

1.Key Laboratory of Horticultural Crops Genetic Improvement, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences

收稿日期:2011-01-30 出版日期:2011-11-01 发布日期:2011-11-07
通讯作者: Correspondence YANG Li-mei, Tel: +86-10-82108756, Fax: +86-10-62174123, E-mail: yanglm@mail.caas.net.cn
基金资助:
This work was supported by the grants from the National High Technology Research and Development Program of China (863 Program, 2008AA10Z155), the National Natural Science Foundation of China (31071697), and the earmarked fund for the Modern Agro-Industry Technology Research System, China (nycytx-35-gw01).

Transformation of Cabbage (Brassica oleracea L. var. capitata) with Bt cry1Ba3 Gene for Control of Diamondback Moth

YI Deng-xia, CUI Lei, LIU Yu-mei, ZHUANG Mu, ZHANG Yang-yong, FANG Zhi-yuan , YANG Li-mei

1.Key Laboratory of Horticultural Crops Genetic Improvement, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences

Received:2011-01-30 Online:2011-11-01 Published:2011-11-07
Contact: Correspondence YANG Li-mei, Tel: +86-10-82108756, Fax: +86-10-62174123, E-mail: yanglm@mail.caas.net.cn
Supported by:
This work was supported by the grants from the National High Technology Research and Development Program of China (863 Program, 2008AA10Z155), the National Natural Science Foundation of China (31071697), and the earmarked fund for the Modern Agro-Industry Technology Research System, China (nycytx-35-gw01).

摘要/Abstract

摘要： To obtain transgenic cabbage line with broad insect resistance, a new synthetic Bacillus thuringiensis cry1Ba3 gene was introduced into white cabbage via Agrobacterium tumefaciens-mediated transformation and 37 transformants were obtained. Polymerase chain reaction (PCR) and Southern blot analyses confirmed that cry1Ba3 was successfully inserted into the genome of cabbage. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated that cry1Ba3 was expressed. Western blot results confirmed the production of insecticidal protein encoded by cry1Ba3. Insect bioassays showed that transgenic cabbages effectively controlled both susceptible and Cry1Ac-resistant diamondback moth (DBM) larvae.

关键词: Bacillus thuringiensis cry1Ba3, cabbage, diamondback moth, resistance

Abstract: To obtain transgenic cabbage line with broad insect resistance, a new synthetic Bacillus thuringiensis cry1Ba3 gene was introduced into white cabbage via Agrobacterium tumefaciens-mediated transformation and 37 transformants were obtained. Polymerase chain reaction (PCR) and Southern blot analyses confirmed that cry1Ba3 was successfully inserted into the genome of cabbage. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated that cry1Ba3 was expressed. Western blot results confirmed the production of insecticidal protein encoded by cry1Ba3. Insect bioassays showed that transgenic cabbages effectively controlled both susceptible and Cry1Ac-resistant diamondback moth (DBM) larvae.

Key words: Bacillus thuringiensis cry1Ba3, cabbage, diamondback moth, resistance

YI Deng-xia, CUI Lei, LIU Yu-mei, ZHUANG Mu, ZHANG Yang-yong, FANG Zhi-yuan , YANG Li-mei. Transformation of Cabbage (Brassica oleracea L. var. capitata) with Bt cry1Ba3 Gene for Control of Diamondback Moth [J]. Journal of Integrative Agriculture, 2011, 10(11): 1693-1700.

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Transformation of Cabbage (Brassica oleracea L. var. capitata) with Bt cry1Ba3 Gene for Control of Diamondback Moth

Transformation of Cabbage (Brassica oleracea L. var. capitata) with Bt cry1Ba3 Gene for Control of Diamondback Moth

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