Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (7): 3090-3093.DOI: 10.1016/j.jia.2026.03.022

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从优良种质到转化平台:打破苦荞转化顽拗性

  

  • 收稿日期:2026-01-09 修回日期:2026-03-10 接受日期:2026-02-06 出版日期:2026-07-20 发布日期:2026-06-09

From elite germplasm to transformation platform: Breaking recalcitrance in Tartary buckwheat

Zhen Wang1*, Tong Su1*, Kaixuan Zhang1, Yuqi He1, Zhirong Wang1, Alexander Betekhtin2, Meiliang Zhou1#   

  1. 1 State Key Laboratory of Crop Gene Resources and Breeding/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China

    2 Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Katowice 40-032, Poland

  • Received:2026-01-09 Revised:2026-03-10 Accepted:2026-02-06 Online:2026-07-20 Published:2026-06-09
  • About author:#Correspondence Meiliang Zhou, E-mail: zhoumeiliang@caas.cn * These authors contributed equally to this study.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (32241042, 32161143005, 32301794, 32401816, 32572314, 32501861, and W2512019), the National Key Research and Development Program of China (2023YFF1002500) and the National Science Centre of Poland, Research Project SONATA BIS 10 (Reg. 2020/38/E/NZ9/00033 awarded to AB).  

摘要:

本研究选取了来自不同群系的20份苦荞种质,并系统评估了其未成熟合子胚的原胚细胞复合体(PECCs)诱导和增殖能力,从而筛选出了具有卓越的PECCs诱导和增殖能力的种质G253。基于G253的形态发生愈伤(MC),成功建立了一个稳定的农杆菌介导的遗传转化平台,获得的愈伤转化系中T-DNA整合的阳性率超过90%,并通过PECCs再生体系获得了转基因植株。此外,还基于苦荞MC开发了一个高效的原生质体瞬时转化系统。本研究为克服苦荞遗传转化的瓶颈、加速其分子育种进程提供了关键的种质资源和技术平台。