转录组变化及VvMYBPA1功能分析揭示葡萄抗旱性的分子机制

doi:10.1016/j.jia.2025.12.028

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (4): 1501-1518.DOI: 10.1016/j.jia.2025.12.028

转录组变化及VvMYBPA1功能分析揭示葡萄抗旱性的分子机制

收稿日期:2025-01-14 修回日期:2025-12-18 接受日期:2025-12-16 出版日期:2026-04-20 发布日期:2026-03-11

Transcriptomic changes and VvMYBPA1 function analysis reveal the molecular mechanism of drought tolerance in grapevine

Shuzhen Jiao^{1, 2}, Yaping Huang¹, Shixiong Lu¹, Han Wang¹, Yanmei Li¹, Juan Mao¹, Baihong Chen^1#

¹ College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China
² School of Enology and Horticulture, Ningxia University, Yinchuan 750021, China

Received:2025-01-14 Revised:2025-12-18 Accepted:2025-12-16 Online:2026-04-20 Published:2026-03-11
About author:Shuzhen Jiao, E-mail: jszhen@nxu.edu.cn; #Correspondence Baihong Chen, E-mail: bhch@gsau.edu.cn
Supported by:
This work was supported by the grants from the Key Project of Natural Science Foundation of Gansu Province, China (22JR5RA831), the National Natural Science Foundation of China (32460725), and the Ningxia Natural Science Foundation Project, China ( 2024AAC05037).

摘要/Abstract

摘要： 干旱胁迫对葡萄植株的生长发育具有显著负面影响。砧木嫁接技术虽广泛用于提升葡萄果实品质及抗旱性，但其遗传调控机制尚未明晰。本研究通过对比干旱胁迫下嫁接苗（阳光玫瑰接穗/1103P砧木）与自根苗（阳光玫瑰）叶片以及嫁接苗和自根苗（1103P）根系的生理及转录组发现：嫁接植株通过提升植物激素水平与抗氧化酶活性、降低过氧化氢及丙二醛含量等方式有效缓解了叶片干旱损伤。转录组分析共鉴定到11,855个叶片差异表达基因和11,197个根部差异基因。基于RNA-seq数据的加权基因共表达网络分析（WGCNA）显示，绿黄、黑色、青绿、鲑红及蓝色模块与干旱胁迫显著相关。通路富集分析表明差异表达的基因主要参与植物激素信号转导和MAPK信号通路，同时检测到916个可能调控干旱胁迫的转录因子基因（TFs），分属不同基因家族。通过12个干旱响应基因的实时荧光定量PCR验证了转录组数据可靠性。此外，拟南芥和葡萄愈伤组织中VvMYBPA1基因的过表达均显著增强抗旱性。本研究为解析葡萄干旱适应性的调控机制提供了新见解。

Abstract:

Drought stress negatively affects grapevine growth and development. Grafting with rootstock is widely used to improve the quality of grape fruits and confer drought stress tolerance, but the underlying genetics and regulatory mechanisms are unclear. Hence, we investigated the physiologic and transcriptomic profiles in the leaves of grafted SM/1103P (SM shoot/1103P root) and self-rooted SM (‘Shine Muscat’) as well as roots of grafted SM/1103P and self-rooted 1103P under drought stress conditions. The results indicated that grafted grapevine effectively attenuated drought damage in grape leaves by increasing phytohormone levels and antioxidant enzyme activities, reducing H₂O₂ and MDA contents. Transcriptomic profiling revealed a total of 11,855 and 11,197 differentially expressed genes (DEGs) were identified in grape leaves and roots respectively. Weighted correlation network analysis (WGCNA) was performed based on the RNA-seq data, and five modules (greenyellow, black, turquoise, salmon and blue) were significantly correlated to drought stress. Pathway analysis showed that DEGs were enriched in the plant hormone signal transduction and MAPK signaling pathway. 916 transcription factor genes (TFs) belonging to different gene families were detected that may participate in regulating the drought stress. Quantitative real-time PCR (qRT-PCR) expression analysis of twelve drought stress responsive DEGs was used to verify the transcriptome data. Furthermore, overexpression of VvMYBPA1 in Arabidopsis thaliana and grape callus tissues improved drought tolerance. Our findings provided new insights into to the regulatory mechanism for improving grapevine adaptation to drought.

Key words: grapevine , physiological change , transcriptome , drought stress , VvMYBPA1

Shuzhen Jiao, Yaping Huang, Shixiong Lu, Han Wang, Yanmei Li, Juan Mao, Baihong Chen. 转录组变化及VvMYBPA1功能分析揭示葡萄抗旱性的分子机制[J]. Journal of Integrative Agriculture, 2026, 25(4): 1501-1518.

Shuzhen Jiao, Yaping Huang, Shixiong Lu, Han Wang, Yanmei Li, Juan Mao, Baihong Chen. Transcriptomic changes and VvMYBPA1 function analysis reveal the molecular mechanism of drought tolerance in grapevine[J]. Journal of Integrative Agriculture, 2026, 25(4): 1501-1518.

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转录组变化及VvMYBPA1功能分析揭示葡萄抗旱性的分子机制

Transcriptomic changes and VvMYBPA1 function analysis reveal the molecular mechanism of drought tolerance in grapevine

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