PuMYB93-PuGSTF12模块调控南红梨花青素积累的分子机制

doi:10.1016/j.jia.2025.12.002

摘要/Abstract

摘要：

果实色泽的形成主要源于花青素的积累。为鉴定与花青素积累相关的候选基因，对‘南果梨’及其红色芽变品种‘南红梨’果皮进行了酚类物质代谢和转录组分析。结果表明，‘南红梨’果皮中的花青素和黄酮醇积累量显著增加。分析发现，‘南红梨’果皮中花青素合成及转运相关基因的表达水平均较高，其中PuGSTF12在两个品种中的差异最为显著。进一步筛选获得了一个新的MYB转录因子PuMYB93，对其沉默后抑制了PuGSTF12的表达及花青素的积累，推测其可能参与花青素的调控过程。在此基础上，通过酵母单杂交、凝胶迁移阻滞及GUS报告基因试验证明了PuMYB93可以结合PuGSTF12的启动子并激活其转录。同时，沉默PuGSTF12抑制了PuMYB93对梨果皮花青素的促进作用，表明PuMYB93位于PuGSTF12上游。以上结果表明，PuMYB93可通过调控花青素转运基因PuGSTF12的表达，进而促进花青素积累，从而揭示了PuMYB93-PuGSTF12模块调控‘南红梨’着色的分子机制。

Abstract:

Anthocyanins are important flavonoid pigments in the coloration of fruits. To identify candidate genes involved in anthocyanin accumulation, metabolic and transcriptome analyses were conducted in ‘Nanguo’ pear and its red sport cultivar ‘Nanhong’ pear. The results showed that ‘Nanhong’ pear had significantly higher anthocyanin and flavonol contents. Additionally, transcriptomic analysis showed that there were significant differences in the expression of genes involved in phenylpropanoid and flavonoid biosynthesis pathways between the two cultivars, with PuGSTF12 being the most upregulated gene in the ‘Nanhong’ cultivar. Further analysis identified a novel MYB transcription factor (PuMYB93), and its silencing repressed PuGSTF12 expression and anthocyanin accumulation, suggesting it plays an essential role in the regulation of anthocyanin biosynthesis. Moreover, yeast one-hybrid analysis, electrophoretic mobility shift assay, and β-glucuronidase assay indicated that PuMYB93 can directly bind to the PuGSTF12 promoter to positively regulate its expression. Additionally, PuGSTF12 silencing suppressed the coloration of PuMYB93-OE peels, suggesting that PuGSTF12 act downstream of PuMYB93. Overall, the findings of this study show that PuMYB93 promotes anthocyanin transport in pears by regulating PuGSTF12 expression to further enhance anthocyanin accumulation.

Key words: anthocyanin , pear , transcriptome analysis , transcription factor , PuMYB93

Huili Sun, Xinyue Wang, Qiuping Ren, Chunyan Liu, Xiaoqian Wang. PuMYB93-PuGSTF12模块调控南红梨花青素积累的分子机制[J]. Journal of Integrative Agriculture, 2026, 25(2): 671-681.

Huili Sun, Xinyue Wang, Qiuping Ren, Chunyan Liu, Xiaoqian Wang. The PuMYB93–PuGSTF12 regulatory module promotes anthocyanin accumulation in ‘Nanhong’ pear fruit[J]. Journal of Integrative Agriculture, 2026, 25(2): 671-681.

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