VqMAPK3-VqERF1B-VqPRs模块在葡萄中赋予对白粉病的抗性

doi:10.1016/j.jia.2025.12.001

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (2): 682-693.DOI: 10.1016/j.jia.2025.12.001

VqMAPK3-VqERF1B-VqPRs模块在葡萄中赋予对白粉病的抗性

收稿日期:2024-10-05 修回日期:2025-12-05 接受日期:2025-09-25 出版日期:2026-02-20 发布日期:2026-01-06

VqMAPK3–VqERF1B–VqPRs module confers resistance against Erysiphe necator in grapevine

Chaohui Yan¹, Juexi Liu¹, Xiaoxuan Wang^1#, Yunfei Wang¹, Yuejin Wang², Jiaping Liang^1#, Qiliang Yang^1#

¹ Faculty of Modern Agricultural Engineering, Kunming University of Science and Technology, Kunming 650500, China
² College of Horticulture, Northwest A&F University, Yangling 712100, China

Received:2024-10-05 Revised:2025-12-05 Accepted:2025-09-25 Online:2026-02-20 Published:2026-01-06
About author:Chaohui Yan, E-mail: ychputao@163.com; #Correspondence Xiaoxuan Wang, E-mail: 112004010002@home.hpu.edu.cn; Jiaping Liang, E-mail: liangjpxaut@163.com; Qiliang Yang, E-mail: yangqilianglovena@163.com
Supported by:
This study is granted by the National Natural Science Foundation of China (52209055, 52379041, and 32272667), the Yunnan Fundamental Research Projects, China (202401AU070197, 202501AW070013, 202501BC070015, and 202501AT070377), the Yunnan Education Department Project, China (2024J0079), the Kunming University of Science and Technology Talent Development Project, China (KKZ3202423161), the Yunnan Key Laboratory of Efficient Utilization of Agricultural Water Resources and Intelligent Control, China (202449CE340014), and the Yunnan Intelligent Water-Fertilizer-Pesticide Integration Technology and Equipment Innovation Team, China (202505AS350025).

摘要/Abstract

摘要：

葡萄白粉菌是严重影响葡萄产量和品质的一种真菌性病原体，对葡萄产业造成重大经济损失。因此，鉴定和表征宿主的抗病机制至关重要。本研究中，我们从中国野生毛葡萄“丹凤-2”中鉴定到一个在葡萄白粉病菌侵染过程中持续高表达的转录因子VqERF1B。在葡萄叶片中瞬时过表达VqERF1B后，发现VqERF1B通过提升PR1、PR2、PR5和PR10等病程相关蛋白基因的转录水平，增强葡萄对白粉病的抗性。相反，VqERF1B基因沉默后，葡萄对白粉病的抗性降低。同时，我们对稳定过表达VqERF1B的转基因拟南芥的抗病性评估表明，VqERF1B通过促进PRs的高表达和活性氧（ROS）的积累来增强对白粉病的抗性。此外，一系列试验证实，磷酸化激酶VqMAPK3与VqERF1B蛋白互作。VqERF1B被证明可直接与VqPRs的启动子结合，从而促进VqPRs表达。有趣的是，VqMAPK3-VqERF1B转录复合物对VqPR启动子的激活活性高于VqERF1B单独作用时，以上结果表明VqMAPK3在VqERF1B促进PR基因表达过程中发挥着正向协同作用。本研究揭示了葡萄抗白粉病调控过程，为葡萄分子育种奠定了基础。

Abstract:

Erysiphe necator is a destructive fungal pathogen that compromises grapevine yield and quality, leading to substantial economic losses. Therefore, elucidating host resistance mechanisms is essential. In this study, we identified an ethylene response factor, VqERF1B, that exhibits sustained high expression during E. necator infection in Chinese wild grape Vitis quinquangularis accession ‘Danfeng-2’. Transient overexpression of VqERF1B in grape leaves enhanced resistance to E. necator by elevating transcript levels of pathogenesis-related (PR) genes, including PR1, PR2, PR5, and PR10. Conversely, silencing VqERF1B resulted in increased susceptibility. Moreover, transgenic Arabidopsis lines stably overexpressing VqERF1B exhibited enhanced resistance to powdery mildew, associated with elevated PR gene expression and increased accumulation of reactive oxygen species (ROS). A series of assays identified VqMAPK3, a phosphorylated mitogen-activated protein kinase, as a direct interactor of VqERF1B. Furthermore, VqERF1B was shown to bind directly to the promoters of VqPRs, thereby activating their transcription. Notably, the VqMAPK3-VqERF1B complex exhibited greater transactivation activity on VqPR promoters than VqERF1B alone, indicating that VqMAPK3 positively modulates VqERF1B-mediated transcription of PR genes. This work advances understanding of the molecular basis of grape resistance to E. necator and provides a foundation for molecular breeding strategies.

Key words: Erysiphe necator , disease resistance , ERF transcription factor , mitogen-activated protein kinase cascade pathway (MAPK) , pathogenesis-related gene

Chaohui Yan, Juexi Liu, Xiaoxuan Wang, Yunfei Wang, Yuejin Wang, Jiaping Liang, Qiliang Yang. VqMAPK3-VqERF1B-VqPRs模块在葡萄中赋予对白粉病的抗性[J]. Journal of Integrative Agriculture, 2026, 25(2): 682-693.

Chaohui Yan, Juexi Liu, Xiaoxuan Wang, Yunfei Wang, Yuejin Wang, Jiaping Liang, Qiliang Yang. VqMAPK3–VqERF1B–VqPRs module confers resistance against Erysiphe necator in grapevine[J]. Journal of Integrative Agriculture, 2026, 25(2): 682-693.

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VqMAPK3-VqERF1B-VqPRs模块在葡萄中赋予对白粉病的抗性

VqMAPK3–VqERF1B–VqPRs module confers resistance against Erysiphe necator in grapevine

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