利用p30-Fc标记的纳米颗粒荧光探针，开发便携式免疫传感器，实现非洲猪瘟病毒抗体的超灵敏、快速即时检测

doi:10.1016/j.jia.2025.11.028

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (6): 2630-2633.DOI: 10.1016/j.jia.2025.11.028

利用p30-Fc标记的纳米颗粒荧光探针，开发便携式免疫传感器，实现非洲猪瘟病毒抗体的超灵敏、快速即时检测

收稿日期:2025-07-22 修回日期:2025-11-15 接受日期:2025-10-23 出版日期:2026-06-20 发布日期:2026-05-07

Rapid and ultrasensitive point-of-care detection of ASFV antibodies using p30-Fc-labeled nanoparticle-based fluorescence strip-assisted portable immunosensor

Yang Yang¹, Jiayang Zheng¹, Yan Zhang¹, Qianming Zhao¹, Yafang Lin^{1, 2}, Junjie Zhang¹, Zongjie Li¹, Ke Liu¹, Beibei Li¹, Donghua Shao¹, Yafeng Qiu¹, Zhiyong Ma^1#, Jianchao Wei^1#

¹ Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
²Key Laboratory of Animal Microbiology of Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China

Received:2025-07-22 Revised:2025-11-15 Accepted:2025-10-23 Online:2026-06-20 Published:2026-05-07
About author:Yang Yang, E-mail: 15776623916@163.com; #Correspondence Jianchao Wei, Tel: +86-21-34510616, Fax: +86-21-54081818, E-mail: jianchaowei@shvri.ac.cn; Zhiyong Ma, Tel: +86-21-34293139, Fax: +86-21-54081818, E-mail: zhiyongma@shvri.ac.cn
Supported by:
The work was supported by grants from the Shanghai Agriculture Applied Technology Development Program, China (X2024-02-08-00-12-F00049, awarded to Yafeng Qiu), the Central Public-interest Scientific Institution Basal Research Fund (CAAS-ZDRW202409, awarded to Jianchao Wei), the Agricultural Science and Technology Innovation Program (CAAS-ZDRW202203, awarded to Zhiyong Ma), and the Nanjing Customs Science and Technology Program of China (2023KJ24, awarded to Jianchao Wei).

摘要/Abstract

摘要：

非洲猪瘟（ASF）是由非洲猪瘟病毒（ASFV）引起的可感染家猪与野猪的一种急性、高致病性、高度接触性传染病。迄今为止，自非洲猪瘟首次报告以来，无商品化安全疫苗或特效治疗药物和方法。因此，迫切需要一种快速简便的诊断方法来监测非洲猪瘟病毒特异性抗体，以控制非洲猪瘟病毒的传播。本研究以ASFV结构蛋白p30和IgG Fc融合蛋白（p30-Fc）为抗原，结合荧光微球作为示踪剂，制备SPA双夹心荧光试纸条特异性检测抗ASFV抗体。结果表明，本研究制备的荧光试纸条对ASFV抗体具有较高的敏感性，灵敏度高达1:2560，具有较高的特异性，与其他猪病毒抗体无交叉反应。同时，荧光条检测结果与市售ELISA试剂盒符合率较高（98%）。并且，其抗体最早可在ASFV感染后4天检测到，因此使该检测方法可作为早期诊断手段。此外，荧光试纸条的检测结果可通过一种廉价且易获得的便携式设备读取。因此，本研究所提出的荧光试纸条是一种快速、灵敏、特异且可视化的检测方法，在未来的ASF疫情监测与防控中具有巨大潜力。

Yang Yang, Jiayang Zheng, Yan Zhang, Qianming Zhao, Yafang Lin, Junjie Zhang, Zongjie Li, Ke Liu, Beibei Li, Donghua Shao, Yafeng Qiu, Zhiyong Ma, Jianchao Wei. 利用p30-Fc标记的纳米颗粒荧光探针，开发便携式免疫传感器，实现非洲猪瘟病毒抗体的超灵敏、快速即时检测[J]. Journal of Integrative Agriculture, 2026, 25(6): 2630-2633.

Yang Yang, Jiayang Zheng, Yan Zhang, Qianming Zhao, Yafang Lin, Junjie Zhang, Zongjie Li, Ke Liu, Beibei Li, Donghua Shao, Yafeng Qiu, Zhiyong Ma, Jianchao Wei. Rapid and ultrasensitive point-of-care detection of ASFV antibodies using p30-Fc-labeled nanoparticle-based fluorescence strip-assisted portable immunosensor[J]. Journal of Integrative Agriculture, 2026, 25(6): 2630-2633.

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利用p30-Fc标记的纳米颗粒荧光探针，开发便携式免疫传感器，实现非洲猪瘟病毒抗体的超灵敏、快速即时检测

Rapid and ultrasensitive point-of-care detection of ASFV antibodies using p30-Fc-labeled nanoparticle-based fluorescence strip-assisted portable immunosensor

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