鸭疫里默氏菌中新型利奈唑胺耐药基因cfr(F)的鉴定

doi:10.1016/j.jia.2025.11.026

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (7): 3094-3098.DOI: 10.1016/j.jia.2025.11.026

鸭疫里默氏菌中新型利奈唑胺耐药基因cfr(F)的鉴定

收稿日期:2025-06-07 修回日期:2025-11-14 接受日期:2025-10-23 出版日期:2026-07-20 发布日期:2026-06-09

Identification of a novel linezolid resistance gene, cfr(F), in Riemerella anatipestifer

Yihua Zhang^{1, 2, 3, 4}, Zhishuang Yang^{1, 2, 3, 4}, Mingshu Wang^{1, 2, 3, 4, 5}, Renyong Jia^{1, 2, 3, 4, 5}, Shun Chen^{1, 2, 3, 4, 5}, Mafeng Liu^{1, 2, 3, 4, 5}, Xinxin Zhao^{1, 2, 3, 4, 5}, Qiao Yang^{1, 2, 3, 4, 5}, Ying Wu^{1, 2, 3, 4, 5}, Shaqiu Zhang^{1, 2, 3, 4, 5}, Juan Huang^{1, 2, 3, 4, 5}, Xumin Ou^{1, 2, 3, 4, 5}, Di Sun^{1, 2, 3, 4, 5}, Bin Tian^{1, 2, 3, 4, 5}, Yu He^{1, 2, 3, 4, 5}, Zhen Wu^{1, 2, 3, 4, 5}, Anchun Cheng^{1, 4, 5, 6#}, Dekang Zhu^{1, 2, 3, 4, 5#}

¹Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
²Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Chengdu 611130, China
³Key Laboratory of Agricultural Bioinformatics, Ministry of Education, Chengdu 611130, China
⁴International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China
⁵Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education, Chengdu 611130, China
⁶Institute of Veterinary Immunology and Green Drugs/Veterinary Department, College of Animal Science/State Key Laboratory of Green Pesticide, Guizhou University, Guiyang 550025, China

Received:2025-06-07 Revised:2025-11-14 Accepted:2025-10-23 Online:2026-07-20 Published:2026-06-09
About author:Yihua Zhang, E-mail: zyh18881577639@163.com; #Correspondence Dekang Zhu, E-mail: zdk24@sicau.edu.cn; Anchun Cheng, E-mail: chenganchun@vip.163.com
Supported by:
This work was supported by the National Key Research and Development Program of China (2023YFD1800200), the Key Program of the National Natural Science Foundation of China (U24A20451), the Earmarked Fund for China Agriculture Research System (CARS-42-17), and the Sichuan Veterinary Medicine and the Drug Innovation Group of China Agricultural Research System (SCCXTD-2025-18).

摘要/Abstract

摘要：

抗生素耐药性仍是人类与兽医学领域面临的最严峻挑战之一。鸭疫里默氏菌是一种重要的禽类病原，已被证实具有多重耐药性。本研究通过全基因组测序，从鸭疫里默氏菌临床菌株RCAD1242中鉴定并表征了一个新型利奈唑胺耐药基因cfr(F)。通过将cfr(F)克隆至敏感株鸭疫里默氏菌ATCC 11845与多杀性巴氏杆菌DY120818并进行药敏试验，证实该基因可介导对酰胺醇类、林可酰胺类、恶唑烷酮类、截短侧耳素及A型链阳菌素的交叉耐药，即PhLOPS_A表型。其氨基酸序列与已报道的cfr基因的相似性仅为32.9%-43.8%。重组菌株对氟苯尼考、利奈唑胺等抗生素的最小抑菌浓度（MIC）提升2-1024倍。目前cfr(F)基因已在中国、韩国和美国被检出，呈现跨地域传播的趋势。鉴于鸭疫里默氏菌在家禽广泛流行并具备可移动遗传元件交换能力，该菌可能成为恶唑烷酮类耐药性向临床重要人类病原菌传播的潜在储存库，构成公共卫生威胁。本研究强调应加强“同一健康（One Health）”框架下的耐药性监测体系，并在动物、环境、人类领域推行科学、审慎的抗菌药物使用策略，以遏制耐药基因的跨物种传播。

Yihua Zhang, Zhishuang Yang, Mingshu Wang, Renyong Jia, Shun Chen, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Xumin Ou, Di Sun, Bin Tian, Yu He, Zhen Wu, Anchun Cheng, Dekang Zhu. 鸭疫里默氏菌中新型利奈唑胺耐药基因cfr(F)的鉴定[J]. Journal of Integrative Agriculture, 2026, 25(7): 3094-3098.

Yihua Zhang, Zhishuang Yang, Mingshu Wang, Renyong Jia, Shun Chen, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Xumin Ou, Di Sun, Bin Tian, Yu He, Zhen Wu, Anchun Cheng, Dekang Zhu. Identification of a novel linezolid resistance gene, cfr(F), in Riemerella anatipestifer[J]. Journal of Integrative Agriculture, 2026, 25(7): 3094-3098.

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鸭疫里默氏菌中新型利奈唑胺耐药基因cfr(F)的鉴定

Identification of a novel linezolid resistance gene, cfr(F), in Riemerella anatipestifer

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