EARLY BUD BREAK 和SHORT VEGETATIVE PHASE 4 基因整合ABA植物激素信号转导通路调控葡萄芽休眠

doi:10.1016/j.jia.2025.05.003

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (12): 4613-4629.DOI: 10.1016/j.jia.2025.05.003

EARLY BUD BREAK 和SHORT VEGETATIVE PHASE 4 基因整合ABA植物激素信号转导通路调控葡萄芽休眠

收稿日期:2024-08-27 修回日期:2025-05-09 接受日期:2025-03-18 出版日期:2025-12-20 发布日期:2025-11-13

EARLY BUD BREAK and SHORT VEGETATIVE PHASE 4 integrate abscisic acid (ABA) plant hormone signaling control of grape bud dormancy

Yang Dong¹, Muhammad Khalil-Ur-Rehman³, Yi Zhang¹, Liyuan Huang¹, Haoran Li¹, Lina Yang⁴, Huan Zheng^1#, Jianmin Tao^{1, 2#}

¹Sanya Institute of Nanjing Agricultural University/College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
²Institute of Horticultural Crops, Xinjiang Academy of Agricultural Science, Urumqi 830001, China
³Department of Horticultural Sciences, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan
⁴Charles River Laboratories International, lnc., Mattawan, Michigan 49071, USA

Received:2024-08-27 Revised:2025-05-09 Accepted:2025-03-18 Online:2025-12-20 Published:2025-11-13
About author:#Correspondence Huan Zheng, E-mail: huanzheng@njau.edu.cn; Jianmin Tao, E-mail: taojianmin@njau.edu.cn
Supported by:
This work was supported by the China Agriculture Research System of MOF and MARA (CARS-29), the Xinjiang Uygur Autonomous Region Tianchi Talent Introduction Program, China, and the Key R&D Technology Commissioner Projects in Hainan Province, China (ZDYF2024KJTPY008).

摘要/Abstract

摘要：

芽休眠是多年生果树的重要适应性特征，使其能够抵御不利的生长环境。该适应策略在植物的生存和繁殖过程中起着重要作用，但其分子机制尚不清楚。本研究鉴定并分析了葡萄中两个转录因子，EARLY BUD BREAK（VvEBB）和SHORT VEGETATIVE PHASE 4（VvSVP4）。研究结果表明，在杨树中过表达VvSVP4后，其作为萌芽负调控因子发挥作用，而VvEBB则作为正调控因子在芽萌发过程中发挥作用。转录组分析显示，与对照植株相比，植物激素信号通路，特别是涉及脱落酸（ABA）、吲哚乙酸（IAA）和细胞分裂素（CK）的信号通路，在VvSVP4（VvSVP4oe）和VvEBB（VvEBBoe）过表达植株中显著富集。此外，ABA、IAA和CK的内源激素水平变化与转录组数据呈正相关。在内休眠阶段，VvSVP4直接促进ABA受体基因VvPYL9的表达，从而维持芽的休眠状态。相反，在生态休眠阶段，VvEBB基因迅速上调表达，并负向调控与蔗糖非发酵-1-相关蛋白激酶亚家族2基因（VvSAPK2）的表达，促进芽休眠的解除。综上所述，本研究全面阐释了VvSVP4 和 VvEBB 基因在休眠和芽萌发中的作用，整合了细胞周期调控和多种激素信号通路的见解。

Abstract:

Bud dormancy is a crucial adaptation for perennial fruit plants, enabling them to withstand unfavorable growth conditions. This adaptive strategy plays a significant role in the survival and reproduction of these plants, yet its molecular basis is not fully understood. In the current study, two transcription factors in grapes, EARLY BUD BREAK (VvEBB) and SHORT VEGETATIVE PHASE 4 (VvSVP4), were identified and examined. The findings demonstrated that, following heterologous transformation in poplar, VvSVP4 functions as a negative regulator, whereas VvEBB acts as a positive regulator in the process of bud-break. Transcriptome analysis showed that plant hormone signaling pathways, specifically those involving abscisic acid (ABA), indole acetic acid (IAA), and cytokinin (CK), were significantly enriched in plants overexpressing VvSVP4 (VvSVP4oe) and VvEBB (VvEBBoe), compared to control plants. Additionally, changes in the endogenous levels of ABA, IAA, and CK were found to be positively correlated with the transcriptome data. During the endodormancy phase, VvSVP4 directly and positively influenced the expression of the ABA receptor gene VvPYL9, thereby maintaining the state of bud dormancy. Conversely, during ecodormancy, the VvEBB gene was rapidly upregulated and negatively impacted the expression of the sucrose nonfermenting 1-related protein kinase subfamily 2 gene (VvSAPK2), facilitating the release from dormancy. In summary, this study offers a comprehensive explanation of the roles of VvSVP4 and VvEBB genes in dormancy and bud break, integrating insights into cell cycle regulation and multiple hormones signaling pathways.

Key words: bud dormancy , cell cycle , grape , hormone , VvSVP4 , VvEBB

Yang Dong, Muhammad Khalil-Ur-Rehman, Yi Zhang, Liyuan Huang, Haoran Li, Lina Yang, Huan Zheng, Jianmin Tao. EARLY BUD BREAK 和SHORT VEGETATIVE PHASE 4 基因整合ABA植物激素信号转导通路调控葡萄芽休眠[J]. Journal of Integrative Agriculture, 2025, 24(12): 4613-4629.

Yang Dong, Muhammad Khalil-Ur-Rehman, Yi Zhang, Liyuan Huang, Haoran Li, Lina Yang, Huan Zheng, Jianmin Tao. EARLY BUD BREAK and SHORT VEGETATIVE PHASE 4 integrate abscisic acid (ABA) plant hormone signaling control of grape bud dormancy[J]. Journal of Integrative Agriculture, 2025, 24(12): 4613-4629.

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EARLY BUD BREAK 和SHORT VEGETATIVE PHASE 4 基因整合ABA植物激素信号转导通路调控葡萄芽休眠

EARLY BUD BREAK and SHORT VEGETATIVE PHASE 4 integrate abscisic acid (ABA) plant hormone signaling control of grape bud dormancy

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