通过CRISPR/Cas9编辑OsFAD2-1高效培育高油酸水稻品种

doi:10.1016/j.jia.2025.04.037

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (12): 4810-4814.DOI: 10.1016/j.jia.2025.04.037

通过CRISPR/Cas9编辑OsFAD2-1高效培育高油酸水稻品种

收稿日期:2025-01-13 修回日期:2025-04-25 接受日期:2025-04-09 出版日期:2025-12-20 发布日期:2025-11-13

Efficient breeding of high oleic rice cultivar by editing OsFAD2-1 via CRISPR/Cas9

Yingying Wu^{1, 2*}, Zhihui Chen^{2, 3, 4*}, Chan Wang^{1, 2}, Yang Xu^{1, 2, 3, 4}, Xia Li^{2, 3, 4}, Jianping Zhu^{2, 3, 4}, Xiaoli Tan¹, Jie Yang^{1, 2, 3, 4#}

¹School of Life Sciences, Jiangsu University, Zhenjiang 212001, China
²Key Laboratory of Germplasm Innovation in Downstream of Huaihe River, Ministry of Agriculture and Rural Affairs/Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
³Zhongshan Biological Breeding Laboratory, Nanjing 210014, China
⁴Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China

Received:2025-01-13 Revised:2025-04-25 Accepted:2025-04-09 Online:2025-12-20 Published:2025-11-13
About author:Yingying Wu, E-mail: m13863275949@163.com; #Correspondence Jie Yang, Tel: +86-25-84390320, E-mail: yangjie168@aliyun.com * These authors contributed equally to this study.
Supported by:
This work was supported by the National Natural Science Foundation of China (32201861) and Ministry of Agriculture and Rural Affairs of China, the Key Research and Development Program of Jiangsu Province, China (BE2022383), and the Zhongshan Biological Breeding Laboratory, China (ZSBBLKY2023-04-1).

摘要/Abstract

摘要：

随着人们对大米营养品质关注度的提升，米糠油作为稻米副产品的高附加值开发方向日益受到重视。水稻种子中油酸含量偏低，限制了米糠油的营养价值和氧化稳定性。已有研究证实脂肪酸去饱和酶基因OsFAD2在油酸代谢中发挥关键作用，但目前在水稻中对其育种利用仍较少，尤其对其农艺性状影响研究有限。本研究以主栽粳稻品种苏垦118为材料，应用CRISPR/Cas9技术敲除OsFAD2-1基因，构建高油酸突变体。通过脂肪酸组分检测发现，突变体中油酸含量显著升高，亚油酸含量下降，脂肪酸组成得到优化。同时，对T₂代材料进行农艺性状与RVA指标分析，结果显示突变体主要农艺性状保持稳定，部分指标表现优于对照。本研究明确了OsFAD2-1对水稻籽粒脂质组成的调控作用，为功能型稻米的分子育种提供了新思路，也为米糠油品质提升及水稻副产物的高值化利用奠定了基础。

Yingying Wu, Zhihui Chen, Chan Wang, Yang Xu, Xia Li, Jianping Zhu, Xiaoli Tan, Jie Yang. 通过CRISPR/Cas9编辑OsFAD2-1高效培育高油酸水稻品种[J]. Journal of Integrative Agriculture, 2025, 24(12): 4810-4814.

Yingying Wu, Zhihui Chen, Chan Wang, Yang Xu, Xia Li, Jianping Zhu, Xiaoli Tan, Jie Yang. Efficient breeding of high oleic rice cultivar by editing OsFAD2-1 via CRISPR/Cas9[J]. Journal of Integrative Agriculture, 2025, 24(12): 4810-4814.

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通过CRISPR/Cas9编辑OsFAD2-1高效培育高油酸水稻品种

Efficient breeding of high oleic rice cultivar by editing OsFAD2-1 via CRISPR/Cas9

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