CmGhc1基因的突变导致甜瓜下胚轴呈现白色

doi:10.1016/j.jia.2025.03.015

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (11): 4242-4254.DOI: 10.1016/j.jia.2025.03.015

CmGhc1基因的突变导致甜瓜下胚轴呈现白色

收稿日期:2024-08-20 修回日期:2025-03-22 接受日期:2025-02-26 出版日期:2025-11-20 发布日期:2025-10-13

Mutation in CmGhc1 confers the white hypocotyl phenotype in melon (Cucumis melo L.)

Yong Yang^{1, 2}, Rong Fan², Xuejun Zhang², Meihua Li², Yongbing Zhang², Hongping Yi², Manrui Ma², Yun Yang², Bin Liu^2#, Xingwang Liu^1#, Huazhong Ren^1#

¹College of Horticulture, China Agricultural University, Beijing 100193, China
²Biological Breeding Laboratory, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2024-08-20 Revised:2025-03-22 Accepted:2025-02-26 Online:2025-11-20 Published:2025-10-13
About author:#Correspondence Bin Liu, E-mail: liubincau@163.com; Xingwang Liu, E-mail: liuxw01@cau.edu.cn; Huazhong Ren, E-mail: renhuazhong@cau.edu.cn
Supported by:
This work was financially supported by the grants from the earmarked fund for Xinjiang Agriculture Research System, China (XJARS-06), the Key Research and Development Task Special Project of Xinjiang, China (2022B02002-3), the Tianshan Talent Training Program, China (2023TSYCLJ0015), the Key Research and Development Program of Xinjiang Uygur Autonomous Region, China (2023B02017).

摘要/Abstract

摘要：

甜瓜是一种全球种植的重要园艺作物，在中国商品化生产中所用的种子近100%为杂交种。幼苗形态，特别是下胚轴颜色，可作为快速评价F₁杂交种子纯度的重要标记。绿色的下胚轴很常见，但白色的下胚轴在甜瓜种质中很少见。在本研究中，从重离子束突变体文库中鉴定了一个下胚轴为白色但叶片为绿色的突变体。遗传分析表明，白色下胚轴受单个隐性基因CmGhc1控制。CmGhc1第5外显子上单碱基缺失导致翻译蛋白提前终止，进而使得HTH-MYB DNA结合域丧失，可能影响了基因功能。CmGhc1定位于细胞核中，酵母双杂交分析和双荧光素酶试验表明该基因具有转录抑制活性。基于单碱基缺失位点，成功开发了KASP标记hc1，可作为甜瓜白色下胚轴种质选育的功能标记。RNA-seq数据显示，CmGhc1显著影响了叶绿素代谢和光合作用相关基因的表达水平。本研究为进一步加深对叶绿素合成代谢的分子调控机制的认知奠定了重要基础，同时为甜瓜白色下胚轴的转育和应用提供了重要标记和理论支持。

Abstract:

Melon is a globally cultivated horticultural crop with a predominantly hybrid commercial seed market in China. Seedling morphology, particularly hypocotyl color, is a valuable trait for rapid F₁ hybrid seed purity assessment. While green hypocotyls are common, white hypocotyls are rare in melon germplasm. This study identified a mutant with white hypocotyl but green leaves from the heavy ion beam mutant library. Genetic analysis revealed that a single recessive gene controlled the white hypocotyl, designated CmGhc1. A single-base deletion in the fifth exon of CmGhc1 led to a truncated CmGhc1 lacking the HTH-MYB DNA binding domain, likely affecting its transcriptional activity. CmGhc1 was localized in the nucleus, and yeast two-hybrid analysis and a dual-LUC assay demonstrated it as a transcription repressor. Furthermore, a KASP marker (hc1) was developed and verified as a functional marker for breeding white hypocotyl germplasms in melon. RNA-seq data revealed that CmGhc1 significantly affected the transcription of genes related to chlorophyll metabolism and photosynthesis in hypocotyl. In summary, these findings contribute to our understanding of chloroplast biogenesis and provide a valuable tool for melon breeding.

Key words: melon , hypocotyl color , fine mapping , functional marker , RNA-seq

Yong Yang, Rong Fan, Xuejun Zhang, Meihua Li, Yongbing Zhang, Hongping Yi, Manrui Ma, Yun Yang, Bin Liu, Xingwang Liu, Huazhong Ren. CmGhc1基因的突变导致甜瓜下胚轴呈现白色[J]. Journal of Integrative Agriculture, 2025, 24(11): 4242-4254.

Yong Yang, Rong Fan, Xuejun Zhang, Meihua Li, Yongbing Zhang, Hongping Yi, Manrui Ma, Yun Yang, Bin Liu, Xingwang Liu, Huazhong Ren. Mutation in CmGhc1 confers the white hypocotyl phenotype in melon (Cucumis melo L.)[J]. Journal of Integrative Agriculture, 2025, 24(11): 4242-4254.

参考文献

Ashburner M, Ball C A, Blake J A, Botstein D, Butler H, Cherry J M, Davis A P, Dolinski K, Dwight S S, Eppig J T, Harris M A, Hill D P, Issel-Tarver L, Kasarskis A, Lewis S, Matese J C, Richardson J E, Ringwald M, Rubin G M, Sherlock G. 2000. Gene ontology: tool for the unification of biology. Nature Genetics, 25, 25–29.

Borovsky Y, Monsonego N, Mohan V, Shabtai S, Kamara I, Faigenboim A, Hill T, Chen S, Stoffel K, Van Deynze A, Paran I. 2019. The zinc‐finger transcription factor CcLOL1 controls chloroplast development and immature pepper fruit color in Capsicum chinense and its function is conserved in tomato. The Plant Journal, 99, 41–55.

Cao Y, Diao Q, Chen Y, Jin H, Zhang Y, Zhang H. 2021. Development of KASP markers and identification of a QTL underlying powdery mildew resistance in melon (Cucumis melo L.) By bulked segregant analysis and RNA-seq. Frontiers in Plant Science, 11, 593207.

Chang J, Zhang F, Qin H, Liu P, Wang J, Wu S. 2021. Mutation of SlARC6 leads to tissue-specific defects in chloroplast development in tomato. Horticulture Research, 8, 127.

Chen C, Wu Y, Li J, Wang X, Zeng Z, Xu J, Liu Y, Feng J, Chen H, He Y, Xia R. 2023. Tbtools-II, a “one for all, all for one” bioinformatics platform for biological big-data mining. Molecular Plant, 16, 1733–1742.

Du M, Zhou K, Liu Y, Deng L, Zhang X, Lin L, Zhou M, Zhao W, Wen C, Xing J, Li C B, Li C. 2020. A biotechnology‐based male‐sterility system for hybrid seed production in tomato. The Plant Journal, 102, 1090–1100.

Duan X, Yuan Y, Real N, Tang M, Ren J, Wei J, Liu B, Zhang X. 2024. Fine mapping and identification of candidate genes associated with powdery mildew resistance in melon (Cucumis melo L.). Horticulture Research, 11, uhae222.

Feder A, Burger J, Gao S, Lewinsohn E, Katzir N, Schaffer A A, Meir A, Davidovich-Rikanati R, Portnoy V, Gal-On A, Fei Z, Kashi Y, Tadmor Y. 2015. A Kelch domain-containing F-box coding gene negatively regulates flavonoid accumulation in muskmelon. Plant Physiology, 169, 1714–1726.

Fitter D W, Martin D J, Copley M J, Scotland R W, Langdale J A. 2002. GLK gene pairs regulate chloroplast development in diverse plant species. The Plant Journal, 31, 713–727.

Gao P, Ma H, Luan F, Song H. 2012. DNA fingerprinting of chinese melon provides evidentiary support of seed quality appraisal. PLoS ONE, 7, e52431.

Hao Y, Song Q, Chen H, Zou H, Wei W, Kang X, Ma B, Zhang W, Zhang J, Chen S. 2010. Plant NAC-type transcription factor proteins contain a NARD domain for repression of transcriptional activation. Planta, 232, 1033–1043.

Islam M R, Hossain M R, Jesse D M I, Jung H, Kim H, Park J, Nou I. 2020. Development of molecular marker linked with bacterial fruit blotch resistance in melon (Cucumis melo L.). Genes, 11, 220.

Kanehisa M, Goto S, Kawashima S, Okuno Y, Hattori M. 2004. The KEGG resource for deciphering the genome. Nucleic Acids Research, 32, D277–D280.

Karim K M R, Rafii M Y, Misran A B, Ismail M F B, Harun A R, Khan M M H, Chowdhury M F N. 2021. Current and prospective strategies in the varietal improvement of chilli (Capsicum annuum L.) specially heterosis breeding. Agronomy, 11, 2217.

Kong Q, Gao L, Cao L, Liu Y, Saba H, Huang Y, Bie Z. 2016. Assessment of suitable reference genes for quantitative gene expression studies in melon fruits. Frontiers in Plant Science, 7, 1178.

Li H, Durbin R. 2009. Fast and accurate short read alignment with burrows-wheeler transform. Bioinformatics, 25, 1754–1760.

Li Z, Xu Y. 2022. Bulk segregation analysis in the NGS era, a review of its teenage years. The Plant Journal, 109, 1355–1374.

Liu H, Jiao J, Liang X, Liu J, Meng H, Chen H, Li Y, Cheng Z. 2016. Map-based cloning, identification and characterization of the w gene controlling white immature fruit color in cucumber (Cucumis sativus L.). Theoretical and Applied Genetics, 129, 1247–1256.

Liang X, Wang J, Cao L, Du X, Qiang J, Li W, Wang P, Hou J, Li X, Mao W, Zhu H, Yang L, Li Q, Hu J. 2024. An allelic variation in the promoter of the LRR-RLK gene, qSS6.1, is associated with melon seed size. Journal of Integrative Agriculture, 23, 2095–3119.

Love M I, Huber W, Anders S. 2014. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biology,15, 550.

Lv Y, Gao P, Liu S, Fang X, Zhang T, Liu T, Amanullah S, Wang X, Luan F. 2022. Genetic mapping and QTL analysis of stigma color in melon (Cucumis melo L.). Frontiers in Plant Science, 13, 865082.

Mckenna A, Hanna M, Banks E, Sivachenko A, Cibulskis K, Kernytsky A, Garimella K, Altshuler D, Gabriel S, Daly M, DePristo M A. 2010. The genome analysis toolkit: A mapreduce framework for analyzing next-generation DNA sequencing data. Genome Research, 20, 1297–1303.

Mo C, Wang H, Wei M, Zeng Q, Zhang X, Fei Z, Zhang Y, Kong Q. 2024. Complete genome assembly provides a high-quality skeleton for pan-NLRome construction in melon. The Plant Journal, 118, 2249–2268.

Nadakuduti S S, Holdsworth W L, Klein C L, Barry C S. 2014. KNOX genes influence a gradient of fruit chloroplast development through regulation of GOLDEN2‐LIKE expression in tomato. The Plant Journal, 78, 1022–1033.

Oren E, Tzuri G, Vexler L, Dafna A, Meir A, Faigenboim A, Kenigswald M, Portnoy V, Schaffer A A, Levi A, Buckler E S, Katzir N, Burger J, Tadmor Y, Gur A. 2019. The multi-allelic APRR2 gene is associated with fruit pigment accumulation in melon and watermelon. Journal of Experimental Botany, 70, 3781–3794.

Paris H S, Hanan A, Baumkoler F. 2013. Another gene affecting fruit and stem color in squash, Cucurbita pepo. Euphytica, 191, 99–107.

Sainz M B, Grotewold E, Chandler V L. 1997. Evidence for direct activation of an anthocyanin promoter by the maize Cl protein and comparison of DNA binding by related Myb domain proteins. The Plant Cell, 9, 611–625.

Tzuri G, Zhou X, Chayut N, Yuan H, Portnoy V, Meir A, Sa’Ar U, Baumkoler F, Mazourek M, Lewinsohn E, Fei Z, Schaffer A A, Li L, Burger J, Katzir N, Tadmor Y. 2015. A ‘golden’ SNP in CmOr governs the fruit flesh color of melon (Cucumis melo). The Plant Journal, 82, 267–279.

Wang J, Dai Y, Pan L, Chen Y, Dai L, Ma Y, Zhou X, Miao W, Hamid M R, Zou X, Liu F, Xiong C. 2025. Fine mapping and identification of CaTTG1, a candidate gene that regulates the hypocotyl anthocyanin accumulation in Capsicum annuum L. Horticultural Plant Journal, 11, 264–274.

Wei M, Huang Y, Mo C, Wang H, Zeng Q, Yang W, Chen J, Zhang X, Kong Q. 2023. Telomere-to-telomere genome assembly of melon (Cucumis melo L. var. inodorus) provides a high-quality reference for meta-QTL analysis of important traits. Horticulture Research, 10, uhad189.

Wu Q, Wu X, Zhang X, Jiang C, Xiao B, Zhang Y, Wang Y, Liu G. 2014. Mapping of two white stem genes in tetraploid common tobacco (Nicotiana tabacum L.). Molecular Breeding, 34,1065–1074.

Yang S, Wang X, Yan W, Zhang Y, Song P, Guo Y, Xie K, Hu J, Hou J, Wu Y, Zhu H, Sun S, Yang L. 2023. Melon yellow-green plant (Cmygp) encodes a Golden2-like transcription factor regulating chlorophyll synthesis and chloroplast development. Theoretical and Applied Genetics, 136, 66.

Ye S, Wang Y, Huang D, Li J, Gong Y, Xu L, Liu L. 2013. Genetic purity testing of F1 hybrid seed with molecular markers in cabbage (Brassica oleracea var. capitata). Scientia Horticulturae, 155, 92–96.

Zhai X, Wu H, Wang Y, Zhang Z, Shan L, Zhao X, Wang R, Liu C, Weng Y, Wang Y, Liu X, Ren H. 2022. The fruit glossiness locus, dull fruit (D), encodes a C2H2-type zinc finger transcription factor, CsDULL, in cucumber (Cucumis sativus L.). Horticulture Research, 9, 146.

Zhang H, Li X, Yu H, Zhang Y, Li M, Wang H, Wang D, Wang H, Fu Q, Liu M, Ji C, Ma L, Tang J, Li S, Miao J, Zheng H, Yi H. 2019. A high-quality melon genome assembly provides insights into genetic basis of fruit trait improvement. iScience, 22, 16–27.

Zhang K, Yuan M, Xia H, He L, Ma J, Wang M, Zhao H, Hou L, Zhao S, Li P, Tian R, Pan J, Li G, Thudi M, Ma C, Wang X, Zhao C. 2022. BSA-seq and genetic mapping reveals AhRt2 as a candidate gene responsible for red testa of peanut. Theoretical and Applied Genetics, 135, 1529–1540.

Zhang L, Huang Z, Wang X, Gao J, Guo Y, Du Y, Hu H. 2016. Fine mapping and molecular marker development of anthocyanin absent, a seedling morphological marker for the selection of male sterile 10 in tomato. Molecular Breeding, 36, 107.

Zhang X, Ling Y, Yang W, Wei M, Wang Z, Li M, Yang Y, Liu B, Yi H, Guo Y, Kong Q. 2023. Fine mapping of a novel QTL DM9.1 conferring downy mildew resistance in melon. Frontiers in Plant Science, 14, 1202775.

Zhao G, Lian Q, Zhang Z, Fu Q, He Y, Ma S, Ruggieri V, Monforte A J, Wang P, Julca I, Wang H, Liu J, Xu Y, Wang R, Ji J, Xu Z, Kong W, Zhong Y, Shang J, Pereira L, et al. 2019. A comprehensive genome variation map of melon identifies multiple domestication events and loci influencing agronomic traits. Nature Genetics, 51, 1607–1615.

Zhu L, Wang Y, Zhang Z, Hu D, Wang Z, Hu J, Ma C, Yang L, Sun S, Li Y. 2022. Chromosomal fragment deletion in APRR2-repeated locus modulates the dark stem color in Cucurbita pepo. Theoretical and Applied Genetics, 135, 4277–4288.

CmGhc1基因的突变导致甜瓜下胚轴呈现白色

Mutation in CmGhc1 confers the white hypocotyl phenotype in melon (Cucumis melo L.)

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