全基因组关联分析揭示GmRGD14正向调控大豆根干重

doi:10.1016/j.jia.2025.03.007

全基因组关联分析揭示GmRGD14正向调控大豆根干重

收稿日期:2024-10-09 修回日期:2025-03-20 接受日期:2024-11-12

A genome-wide association study revealed that GmRGD14 positively regulates the root dry weight in soybeans

Kaili Ren^1*, Jialuo Chen^1*, Xuan Cui¹, Xiao Li¹, Dezhou Hu^{1, 3}, Zhongyi Yang¹, Yu’e Zhang¹, Yuming Yang^{1, 4}, Deyue Yu¹^#, Hui Wang^{1, 2#}

¹National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China
²Zhongshan Biological Breeding Laboratory (ZSBBL), Nanjing 210095, China
³College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
⁴School of Agriculture, Henan Institute of Science and Technology, Xinxiang 453003, China

Received:2024-10-09 Revised:2025-03-20 Accepted:2024-11-12
About author:#Correspondence Hui Wang, Tel: +86-25-84399527, Fax: +86-25-84396410, E-mail: wanghui0@njau.edu.cn; Deyue Yu, Tel: +86-25-84396410, Fax: +86-25-84396410, E-mail: dyyu@njau.edu.cn. * These authors contributed equally to this work.
Supported by:
The funding for this research was provided by Biological Breeding-National Science and Technology Major Project (2023ZD04070), Zhongshan Biological Breeding Laboratory (ZSBBL-KY2023-03), and the National Natural Science Foundation of China (32101742, 32072080, 32090065).

摘要/Abstract

摘要： 根系对作物的生长发育、产量形成及逆境适应具有重要作用。尽管已有研究鉴定出多个与大豆根系形态特征相关的遗传位点，但相关调控基因发掘不足。本研究通过全基因组关联分析鉴定出7个包含与大豆根干重显著相关的稳定SNP的数量性状位点（QTL），其中qRDW14-2位点显著性最高。在该位点中，编码溶血磷脂酸酰基转移酶LPAT4的GmRGD14基因被确定为候选基因，该基因位于显著SNP S14_6521715所在的block63区域。GmRGD14在大豆根组织中高表达，其拟南芥同源基因突变体lpat4侧根数目高于对照Col-0。GmRGD14主要定位于细胞膜和内质网。在拟南芥中异源过表达GmRGD14可显著增加侧根数量，与atlpat4突变体表型一致。在大豆毛状根中过表达GmRGD14可显著增加总根长、根尖数、根表面积和根体积，而通过RNA干扰敲低该基因的表达量则呈现相反表型。GmRGD14在野生大豆中具有丰富的遗传变异，并在大豆驯化过程中被逐渐利用。总之，本研究揭示了一个新的调控大豆根系生长的关键基因GmRGD14，为培育强根系的优异大豆品种提供了新的分子靶标。

Abstract:

Roots are vital for crop growth, development, yield and tolerance to various types of environmental stress. Numerous genetic loci associated with soybean root morphological traits have been identified, but few genes associated with these traits have been identified. In this study, seven quantitative trait loci (QTLs) containing stable SNPs significantly associated with the root dry weight in soybeans were identified through a genome-wide association study. Among these QTLs, qRDW14-2 presented the greatest significance. In qRDW14-2, the gene GmRGD14, encoding the lysophosphatidic acid acyltransferase LPAT4, was identified as a candidate. GmRGD14, in block63, which contained the significant SNP S14_6521715, had the highest expression level in soybean roots, and its Arabidopsis homologous mutant lpat4 presented more lateral roots than did the control Col-0. GmRGD14 was localized primarily to the cell membrane and endoplasmic reticulum. The heterologous overexpression of GmRGD14 in Arabidopsis significantly increased the lateral root number, which was similar to the phenotype of atlpat4. Furthermore, overexpression of GmRGD14 resulted in a greater total root length, root tip number, root surface area and root volume in the hairy roots of transgenic soybean plants than in those of WT soybean plants, whereas knockdown of the gene via RNA interference in soybean hairy roots resulted in the opposite phenotype. GmRGD14, which is highly genetically variable in wild soybean, has been gradually utilized during soybean domestication. Overall, this study revealed that GmRGD14 is a new key gene that plays a role in root growth, providing a new genetic target for breeding elite soybean varieties with strong root systems.

Key words: soybean , root dry weight , QTL , GmRGD14

. 全基因组关联分析揭示GmRGD14正向调控大豆根干重[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2025.03.007.

Kaili Ren, Jialuo Chen, Xuan Cui, Xiao Li, Dezhou Hu, Zhongyi Yang, Yu’e Zhang, Yuming Yang, Deyue Yu, Hui Wang. A genome-wide association study revealed that GmRGD14 positively regulates the root dry weight in soybeans[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2025.03.007.

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