Journal of Integrative Agriculture

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TRPA1通道调控桃蚜的温度偏好

  

  • 修回日期:2025-02-24

The TRPA1 channel regulates temperature preference in the green peach aphid Myzus persicae

Lulu Yang1, 2, Tianyu Huang1, Jie Shen2, Bing Wang1#, Guirong Wang1, 3#   

  1. 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
    2 Department of Entomology and MOA Key Laboratory for Monitory and Green Control of Crop Pest, China Agricultural University, Beijing 100193, China
    3 Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
  • Revised:2025-02-24
  • About author:Lulu Yang, E-mail: 82101219117@caas.cn; #Correspondence Bing Wang, E-mail: wangbing02@caas.cn; Guirong Wang, E-mail: wangguirong@caas.cn
  • Supported by:

    This study was funded by the Natural Science Foundation of National Natural Science Foundation of China (31872039 and 32472553), Major special projects for green pest control (110202201017(LS-01)), a grant from the Shenzhen Science and Technology Program (KQTD20180411143628272) and the Agricultural Science and Technology Innovation Program. 

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摘要：

瞬时受体电位（transient receptor potential，TRP）通道是一类与昆虫热感应密切相关的离子通道蛋白。它们参与识别环境温度，在昆虫的生存繁衍过程中发挥着重要作用。本研究鉴定并克隆了桃蚜Myzus persicae中TRPA亚家族TRPA1基因两个剪切型MperTRPA1(A)和MperTRPA1(B)，并通过实时荧光定量PCR技术分析了它们在桃蚜不同组织中的表达模式。随后，在爪蟾卵母细胞系统中表达了MperTRPA1基因的两个剪切型，并利用双电极电压钳技术对它们的体外功能进行了深入研究。此外，通过RNA干扰技术结合行为选择试验，评估桃蚜对温度梯度反应的变化，进一步明确MperTRPA1基因在桃蚜温度适应机制中的作用。研究结果显示，MperTRPA1基因在桃蚜成虫多种组织中均有广泛表达。具体而言，MperTRPA1(A)在口器中表达量较高，而MperTRPA1(B)则主要在触角中表达。功能研究进一步揭示，MperTRPA1两个剪切型均能被20℃至45℃的温度范围所激活，且表现出非脱敏特性。深入研究发现，与MperTRPA1(A)相比，MperTRPA1(B)具有更高的电流值和热敏感性（以Q10系数值衡量）。在行为实验中，与野生型和dsGFP处理的桃蚜相比，MperTRPA1基因表达水平敲低的桃蚜对最适温度区域的偏好明显减弱，倾向于分布在更高的温度区域。综上所述，本研究结果阐明了热传导受体MperTRPA1在介导桃蚜适应性温度感知过程中的分子机制。

Abstract:

Transient receptor potential (TRP) channels are a class of ion channel proteins that are closely related to thermosensation in insects. They are involved in detecting the ambient temperature and play vital roles in insect survival and reproduction. In this study, we identified and cloned two variants of the TRPA subfamily gene in Myzus persicae, MperTRPA1(A) and MperTRPA1(B), and analyzed their tissue expression by real-time quantitative PCR. Subsequently, these two variants of MperTRPA1 were expressed in the Xenopus oocyte system, and their functions were investigated using the two-electrode voltage clamp technique. The role of the MperTRPA1 gene in temperature adaptation of M. persicae was further determined by RNA interference and a behavioral choice assay to evaluate responses to temperature gradients. The results showed that the MperTRPA1 gene is widely expressed in tissues of M. persicae, with MperTRPA1(A) highly expressed in the mouthparts and MperTRPA1(B) mainly expressed in the antennae. The functional characterization results showed that both variants of MperTRPA1 could be activated and were not desensitized when the temperature increased from 20 to 45°C. The current value and thermal sensitivity (coefficient Q10 value) of MperTRPA1(B) were significantly higher than those of MperTRPA1(A). When the MperTRPA1 gene was knocked down, the behavioral preference of M. persicae for the optimal temperature was reduced and tended to be at a higher temperature, showing a shift in the temperature adaptation range compared to both the wild type and dsGFP-treated M. persicae. In summary, our results elucidated the molecular mechanism of adaptive temperature perception in M. persicae mediated by the thermal sensor MperTRPA1.

Key words: TRP channel , TRPA1 , Myzus persicae , thermosensation , thermal preference