中国野生毛葡萄转录因子NAC17在植物抗病抗逆中的作用

doi:10.1016/j.jia.2025.02.035

摘要/Abstract

摘要：

芪类化合物是一种天然的植物抗毒素，参与了植物对环境中各种生物和非生物胁迫的响应。芪合成酶（STILBENE SYNTHASE，STS）是葡萄合成白藜芦醇的关键酶。然而，调控STS基因表达的机制尚不清楚。在本研究中，我们报道了一个中国野生毛葡萄的NAC转录因子VqNAC17，可以提高转基因拟南芥对盐胁迫、干旱胁迫和丁香假单胞菌（Pst DC3000）的抗性。此外，利用酵母双杂交和BiFC验证了转录因子VqNAC17与VqMYB15之间的相互作用。在转基因拟南芥中，VqNAC17通过与VqMYB15相互作用影响芪类化合物的合成，参与植物免疫。酵母单杂交和LUC瞬时表达实验结果发现，VqNAC17也可以结合VqMYB15的启动子。这些结果表明，VqNAC17是通过与VqMYB15相互作用的方式促进STS表达的关键调控因子。

Abstract:

Stilbenes, natural plant phytoalexin, are involved in the response of various biotic and abiotic stresses in plant environment. STILBENE SYNTHASE (STS) is the key enzyme regulating resveratrol synthesis in grapevine. However, the regulatory mechanism of STS genes expression remains unclear. In this study, we reported a NAC transcription factor, VqNAC17, in Vitis quinquangularis, which can improve plant resistance to salt stress, drought stress and Pseudomonas syringae pv. Tomato DC3000 (Pst DC3000) in transgenic Arabidopsis thaliana. Besides, the interaction between the transcription factor VqNAC17 and VqMYB15 was confirmed using yeast two-hybrid and BiFC. In transgenic A. thaliana, VqNAC17 participates in plant immunity through interaction with VqMYB15 to affect the stilbene synthesis. Furthermore, the experimental results of yeast one-hybrid assay and LUC transient expression assay found that VqNAC17 can also bind to the promoter of VqMYB15. These results indicate that VqNAC17 is a key regulator that can promote the expression of STS by interacting with VqMYB15.

Key words: Vitis quinquangularis , VqNAC17 , VqMYB15 , protein interaction , STS

. 中国野生毛葡萄转录因子NAC17在植物抗病抗逆中的作用[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2025.02.035.

Xiaolin Liu, Jie Zhu, Ruixiang Li, Yang Feng, Qian Yao, Dong Duan. The role of the transcription factor NAC17 in enhancing plant resistance and stress tolerance in Vitis quinquangularis[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2025.02.035.

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