通过全基因组关联分析挖掘小麦抗叶锈病新基因TaRLK-1B

doi:10.1016/j.jia.2025.02.028

摘要/Abstract

摘要：

叶锈病是一种对小麦极具破坏性的叶部病害，对全球小麦生产造成了严重威胁。在本研究中，对来自回交-互交-巢式关联作图（AB-NAMIC）群体的590份材料进行了成株期抗叶锈病表型鉴定，结合660K基因型数据进行全基因组关联分析（GWAS），鉴定到位于1B、2A、2B和7D染色体上的显著数量性状位点（QTL），并定位了位于1B染色体上的候选基因TaRLK-1B。基于TaRLK-1B单倍型开发的CAPS标记能够有效区分抗病和感病品种。该基因编码一种膜定位的富含亮氨酸重复序列的类受体蛋白激酶（LRR-RLK），受到叶锈菌侵染诱导表达。在小麦中敲除该基因导致抗叶锈病能力降低，进一步证实了其作为正向调控因子在防御叶锈病中的作用。此外，TaRLK-1B可能与类受体胞质激酶TaRLCK1B相互作用，从而促进免疫信号转导。研究结果还表明，聚合多个抗病QTL可以显著提高小麦的叶锈病抗性。本研究为抗病基因提供了新的见解，并提供了有价值的QTL信息，有助于小麦抗病分子标记辅助育种。

Abstract:

Leaf rust is a highly destructive foliar disease in wheat, causing major constraints in wheat production worldwide. In this study, we conducted a comprehensive assessment of adult plant resistance to leaf rust in 590 accessions from the advanced backcross-nested association mapping plus inter-crossed (AB-NAMIC) population. We used 660K genotype data to perform a genome-wide association study (GWAS), identifying significant quantitative trait loci (QTLs) on chromosomes 1B, 2A, 2B, and 7D, with particular emphasis on the candidate gene TaRLK-1B on chromosome 1B. A cleaved amplified polymorphic sequence (CAPS) marker was developed based on TaRLK-1B haplotypes and effectively differentiated between resistant and susceptible varieties. This gene encodes a membrane-localized leucine-rich repeat receptor-like kinase (LRR-RLK) that is upregulated in response to the fungal infection that causes leaf rust. Targeted knockout of TaRLK-1B in wheat led to reduced resistance to leaf rust, underscoring its essential role as a positive regulator in defense against this disease. Additionally, we propose that TaRLK-1B interacts with the receptor-like cytoplasmic kinase TaRLCK1B, potentially facilitating immune signal transduction. Our findings also demonstrate that pyramiding minor effect QTLs significantly increases resistance to leaf rust. This study provides novel insights into resistance genes and valuable QTL information, which could improve marker-assisted wheat breeding efforts.

Key words: GWAS , LRR-RLK , , resistance gene , , leaf rust , , wheat

. 通过全基因组关联分析挖掘小麦抗叶锈病新基因TaRLK-1B[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2025.02.028.

Shujuan Liu, Li Zhao, Chenyang Hao, Yuxue Pan, Mengjiao Guo, Yilin Huang, Haixia Liu, Jian Hou, Zaifeng Li, Tian Li, Xinhong Chen, Xueyong Zhang. TaRLK-1B: A novel wheat gene conferring resistance to leaf rust revealed by a genome-wide association study[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2025.02.028.

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